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Patent US4196185A successfully refreshed from Google Patents.
US4196185A
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Immunoassay for phencyclidine
Assignee: Roche Diagnostics Corp
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Filing: 1978-06-05
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Granted: 1980-04-01
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Expires: 1998-06-05
Abstract
Abstract An improved immunoassay for detecting the drug of abuse phencyclidine is described. This immunoassay is characterized in utilizing as reagents (i) an antibody elicited with a novel immunogen comprising 4-[(1-piperidinyl)cyclohexyl] benzoic acid covalently bonded to an immunogenic carrier material through the carboxyl group and (ii) a novel radiolabeled derivative of phencyclidine, namely 125I N-(4-hydroxy-2-phenylethyl)-4-[1-(1-piperidinyl)cyclohexyl] benzamide.
Inventors
- 👤 Antonino Focella
- 👤 John E. Heveran
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 13:15
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/94; G01N33/9486; C; C07; C07D; C07D211/00; C07D211/04; C07D211/06; C07D211/08; C07D211/18; Y; Y10; Y10S; Y10S436/00; Y10S436/804; Y10S436/815
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
2 words
N-(4-hydroxy-2-phenylethyl)-4-[1-(1-piperidinyl)cyclohexyl] benzamide.
Claim 2
Dependent Claim
2 words
N-(4-hydroxy-2-phenylethyl)-4-[1-(1-piperidinyl)cyclohexyl] benzamide.
Claim 3
Dependent Claim
4 words
125 I-N-(4-hydroxy-2-phenylethyl)-4-[1-(1-piperidinyl) cyclohexyl] benzamide.
Claim 4
Dependent Claim
4 words
125 I-N-(4-hydroxy-2-phenylethyl)-4-[1-(1-piperidinyl) cyclohexyl] benzamide.
Claim 5
Dependent Claim
19 words
An immunogen consisting of 4-[1-(piperidinyl) cyclohexyl] benzoic acid covalently liked through its carboxyl group to an immunogenic carrier material.
Claim 6
Dependent Claim
19 words
An immunogen consisting of 4-[1-(piperidinyl) cyclohexyl] benzoic acid covalently liked through its carboxyl group to an immunogenic carrier material.
Claim 7
Dependent Claim
14 words
The immunogen of claim 3 wherein said immunogenic carrier material is bovine serum albumin.
Claim 8
Dependent Claim
87 words
A method for the assay of phencyclidine in a sample which method comprises mixing said sample with known amounts of a 125 I-labelled phencyclidine derivative and an antibody which will selectively complex with phencyclidine, said antibody being elicited in the blood of a host animal by immunization with the immunogen of claim 3, separating antibody bound antigen from free antigen and then measuring the radioactivity of either the free or bound antigen and comparing said value to values obtained previously with samples containing known amounts of phencyclidine.
Claim 9
Dependent Claim
15 words
The method of claim 5 wherein said 125 I-labelled derivative is 125 I-N-(4-hydroxy-2-phenylethyl)-4-[1-(-piperidinyl) cyclohexyl] benzamide.
Claim 10
Dependent Claim
28 words
The method of claim 6 wherein the antibody bound antigen is separated from free antigen by precipitation by ammonium sulfate, the precipitate is centrifuged and the supernatant counted.
Claim 11
Dependent Claim
36 words
The method of claim 7 wherein phencyclidine is assayed qualitatively by comparing the counts obtained to a positive control, whereby counts equal or greater to said control value indicates the presence of phencyclidine in the sample.
Claim 12
Dependent Claim
19 words
The method of claim 7 wherein phencyclidine is assayed quantitatively by comparing the counts obtained to a standard curve.
References Cited (Backward Citations)
US4000143A
Cited
US4038268A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-04-01 | 1983-10-01 | 1984-04-01 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-04-01 | 1987-10-01 | 1988-04-01 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-04-01 | 1991-10-01 | 1992-04-01 | $7700.00 | Window Closed / Lapsed |