Patent US4235962A successfully refreshed from Google Patents.
US4235962A
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Combination kit for transaminase assay of a body fluid
Assignee: Dow Chemical Co
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Filing: 1978-08-21
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Granted: 1980-11-25
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Expires: 1998-08-21
Abstract
Abstract A kit for the determination of transaminase present in body fluid having a first reagent containing aspartate, a second reagent containing alanine, and a third reagent containing 2-oxoglutarate substrate in combination with a secondary reaction system containing reduced nicotinamide adenine dineucleotide.
Inventors
- 👤 James S. Sanderson
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 08:07
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/48; C12Q1/52; Y; Y10; Y10S; Y10S435/00; Y10S435/81
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
166 words
A reagent kit for the determination of transaminase present in a body fluid comprising a first reagent containing aspartate, a second reagent containing alanine, and a third reagent containing 2-oxoglutarate, reduced nicotinamide adenine dinucleotide (NADH), malate dehydrogenase (MDH), and lactate dehydrogenase (LDH) wherein the amounts of each reagent used are sufficient to provide, upon direct mixing of said first reagent with said third reagent, a resulting mixture containing at least 35 millimolar concentration of aspartate, from about 0.2 to about 35 millimolar concentration of 2-oxoglutarate, from about 0.5 to about 5 millimolar concentration of reduced NADH, at least 175 units/liter of LDH and at least 140 units/liter of MDH and upon direct mixing of, said second reagent with said third reagent, to provide a resulting mixture containing at least 130 millimolar alanine, from about 0.2 to about 35 millimolar concentration of 2-oxoglutarate, from about 0.5 to about 5 millimolar concentration of reduced NADH, at least 175 units/liter of LDH and at least 140 units/liter of MDH.
Claim 2
Dependent Claim
166 words
A reagent kit for the determination of transaminase present in a body fluid comprising a first reagent containing aspartate, a second reagent containing alanine, and a third reagent containing 2-oxoglutarate, reduced nicotinamide adenine dinucleotide (NADH), malate dehydrogenase (MDH), and lactate dehydrogenase (LDH) wherein the amounts of each reagent used are sufficient to provide, upon direct mixing of said first reagent with said third reagent, a resulting mixture containing at least 35 millimolar concentration of aspartate, from about 0.2 to about 35 millimolar concentration of 2-oxoglutarate, from about 0.5 to about 5 millimolar concentration of reduced NADH, at least 175 units/liter of LDH and at least 140 units/liter of MDH and upon direct mixing of, said second reagent with said third reagent, to provide a resulting mixture containing at least 130 millimolar alanine, from about 0.2 to about 35 millimolar concentration of 2-oxoglutarate, from about 0.5 to about 5 millimolar concentration of reduced NADH, at least 175 units/liter of LDH and at least 140 units/liter of MDH.
Claim 3
Dependent Claim
31 words
The kit of claim 1 wherein sufficient pyridoxal phosphate is present in said third reagent to reactivate substantially all apo-transaminase in a sample of body fluid tested for total transaminase activity.
Claim 4
Dependent Claim
11 words
The kit of claim 1 wherein said third reagent is lyophilized.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-11-25 | 1984-05-26 | 1984-11-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-11-25 | 1988-05-26 | 1988-11-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-11-25 | 1992-05-26 | 1992-11-25 | $7700.00 | Window Closed / Lapsed |