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US4283494A
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Microbial lipase, process for its preparation and microbiologically pure culture therefor
Assignee: Meito Sangyo KK
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Filing: 1978-04-26
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Granted: 1981-08-11
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Expires: 1998-04-26
Abstract
Abstract A microbial lipase which has (i) an optimal pH for activity of about 9±0.5, (ii) an optimal temperature for activity of about 40° C. to about 48° C., (iii) a lipase activity to be activated by bile salts, (iv) a cholesterol esterase activity, and (v) a molecular weight of about 30×10 4 to about 40×10 4 ; a process for its preparation; and microbiologically pure culture therefor.
Inventors
- 👤 Yoshitaka Kokusho
- 👤 Haruo Machida
- 👤 Shinjiro Iwasaki
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 23:52
CPC Classifications
C; C12; C12N; C12N1/00; C12N1/20; C12N1/205; C12N9/00; C12N9/14; C12N9/16; C12N9/18; C12N9/20; C12R; C12R2001/00; C12R2001/01; C12R2001/05; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/829
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
57 words
A microbial lipase which has (i) an optimal pH for activity of about 9±0.5, (ii) an optimal temperature for activity of about 40° C. to about 48° C., (iii) a lipase activity to be activated by bile salts, (iv) a cholesterol esterase activity, and (v) a molecular weight of about 30×10 4 to about 40×10 4 .
Claim 2
Dependent Claim
57 words
A microbial lipase which has (i) an optimal pH for activity of about 9±0.5, (ii) an optimal temperature for activity of about 40° C. to about 48° C., (iii) a lipase activity to be activated by bile salts, (iv) a cholesterol esterase activity, and (v) a molecular weight of about 30×10 4 to about 40×10 4 .
Claim 3
Dependent Claim
14 words
The microbial lipase of claim 1 which further has (vii) a lipoprotein lipase activity.
Claim 4
Dependent Claim
30 words
The microbial lipase of claim 1 which has a cholesterol esterase activity of at least 300 U/g in the crude state and at least 6,600 U/g in the purified state.
Claim 5
Dependent Claim
38 words
The microbial lipase of claim 1 which further shows (viii) an activity inhibition of at least about 50% by sodium ascorbate in a concentration of 10 -2 mole and monoiodoacetic acid in a concentration of 10 -3 mole.
Claim 6
Dependent Claim
27 words
The microbial lipase of claim 1 which is produced by a microorganism of genus Alcaligenes having the ability to produce the microbial lipase described in claim 1.
Claim 7
Dependent Claim
21 words
The microbial lipase of claim 5 wherein the microorganism has characteristics that permit its identification as Alcaligenes sp. PL-679 Meito strain.
Claim 8
Dependent Claim
24 words
The microbial lipase of claim 6 wherein the microorganism is Alcaligenes sp. PL-679 Meito FERM-P No. 3783 Alcaligenes sp. PL-679 Meito ATCC 31371 strain.
Claim 9
Dependent Claim
74 words
A process for preparing the microbial lipase of claim 1, which comprises aerobically cultivating a microorganism of the genus Alcaligenes having the ability to produce the microbial lipase of claim 1 in a nutrient culture medium containing a carbon source, a nitrogen source and minerals at a pH of about 5.5 to about 11 and a temperature of about 5° to about 40° C., and recovering the microbial lipase from the resulting culture broth.
Claim 10
Dependent Claim
15 words
The process of claim 8 wherein the culture medium further contains a nonionic surface-active agent.
Claim 11
Dependent Claim
30 words
The process of claim 9 wherein the amount of the nonionic surface-active agent is about 0.01 to about 2% by weight based on the weight of the nutrient culture medium.
Claim 12
Dependent Claim
120 words
A biologically pure culture of Alcaligenes sp. PL-679 Meito, ATCC 31371 strain which has the ability to produce a microbial lipase capable of being activated by bile salts and characterized by having (i) an optimal pH for activity of about 9±0.5, (ii) an optimal temperature for activity of about 40° C. to about 48° C., (iii) a lipase activity to be activated by bile salts, (iv) a cholesterol esterase activity, and (v) a molecular weight of about 30×10 4 to about 40×10 4 , by aerobic fermentation in a nutrient culture medium containing a carbon source, a nitrogen course and minerals at a pH of about 5.5 to about 11 and a temperature of about 5° to about 40° C.
Claim 13
Dependent Claim
120 words
A biologically pure culture of Alcaligenes sp. PL-679 Meito, ATCC 31371 strain which has the ability to produce a microbial lipase capable of being activated by bile salts and characterized by having (i) an optimal pH for activity of about 9±0.5, (ii) an optimal temperature for activity of about 40° C. to about 48° C., (iii) a lipase activity to be activated by bile salts, (iv) a cholesterol esterase activity, and (v) a molecular weight of about 30×10 4 to about 40×10 4 , by aerobic fermentation in a nutrient culture medium containing a carbon source, a nitrogen course and minerals at a pH of about 5.5 to about 11 and a temperature of about 5° to about 40° C.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-08-10 | 1985-02-09 | 1985-08-11 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-08-10 | 1989-02-09 | 1989-08-11 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-08-10 | 1993-02-09 | 1993-08-11 | $7700.00 | Window Closed / Lapsed |