Patent US4287305A successfully refreshed from Google Patents.
US4287305A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Microorganism immobilization
Assignee: United States Department of Energy
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Filing: 1976-06-30
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Granted: 1981-09-01
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Expires: 1996-06-30
Abstract
Abstract Live metabolically active microorganisms are immobilized on a solid support by contacting particles of aggregate material with a water dispersible polyelectrolyte such as gelatin, crosslinking the polyelectrolyte by reacting it with a crosslinking agent such as glutaraldehyde to provide a crosslinked coating on the particles of aggregate material, contacting the coated particles with live microorganisms and incubating the microorganisms in contact with the crosslinked coating to provide a coating of metabolically active microorganisms. The immobilized microorganisms have continued growth and reproduction functions.
Inventors
- 👤 Alicia L. Compere
- 👤 William L. Griffith
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 22:52
CPC Classifications
C; C12; C12P; C12P7/00; C12P7/40; C12P7/56; C12N; C12N1/00; C12N11/00; C12N11/02; C12N11/08; C12N11/082; C12N11/087; C12N11/14
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
94 words
A process for producing an aggregate support material coated with a crosslinked coating, said coating having live, metabolically active microorganisms attached thereto, comprising the steps of: contacting a particle of aggregate material with a water dispersible gelatin; crosslinking said gelatin by reacting it with a glutaraldehyde to provide a crosslinked coating on said particle of aggregate material; then contacting said coated particle with live microorganisms; and then incubating said microorganisms in contact with said crosslinked coating to provide a coating having metabolically active microorganisms attached thereto, said microorganisms having continued growth and reproductive functions.
Claim 2
Dependent Claim
94 words
A process for producing an aggregate support material coated with a crosslinked coating, said coating having live, metabolically active microorganisms attached thereto, comprising the steps of: contacting a particle of aggregate material with a water dispersible gelatin; crosslinking said gelatin by reacting it with a glutaraldehyde to provide a crosslinked coating on said particle of aggregate material; then contacting said coated particle with live microorganisms; and then incubating said microorganisms in contact with said crosslinked coating to provide a coating having metabolically active microorganisms attached thereto, said microorganisms having continued growth and reproductive functions.
Claim 3
Dependent Claim
32 words
The process according to claim 1 wherein said particle of aggregate material is composed of a material selected from the group consisting of metals, porcelain, stoneware, glass, polyethylene, polypropylene, nylon and styrene.
Claim 4
Dependent Claim
11 words
The method of claim 1 in which said microorganisms are anaerobic.
Claim 5
Dependent Claim
26 words
An aggregate support material coated with a glutaraldehyde crosslinked gelatin coating having live metabolically active microorganisms attached thereto, said microorganisms having continued growth and reproductive functions.
Claim 6
Dependent Claim
26 words
An aggregate support material coated with a glutaraldehyde crosslinked gelatin coating having live metabolically active microorganisms attached thereto, said microorganisms having continued growth and reproductive functions.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-08-31 | 1985-03-02 | 1985-09-01 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-08-31 | 1989-03-02 | 1989-09-01 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-08-31 | 1993-03-02 | 1993-09-01 | $7700.00 | Window Closed / Lapsed |