Patent US4295853A successfully refreshed from Google Patents.
US4295853A
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Method for measuring peroxidic substances
Assignee: Fujifilm Wako Pure Chemical Corp
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Filing: 1978-12-29
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Granted: 1981-10-20
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Expires: 1998-12-29
Abstract
Abstract Peroxidic substances in a sample, e.g. one or more peroxidic substances in fats and oils or in an organic material, lipoperoxides in a living sample, etc. can be determined, directly in a sample or after extracting the lipoperoxides from a living sample with a solvent, by contacting the peroxidic substances with an oxidizable color reagent in the presence of a metallic compound produced from a metal which is able to have two or more valences to produce color, and measuring degree of coloring colorimetrically.
Inventors
- 👤 Shizuka Kasahara
- 👤 Kazuhiko Yamanishi
- 👤 Toshiro Hanada
- 👤 Kuniaki Tokuda
- 👤 Shinzo Kobatake
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 18:55
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/52; C; C07; C07D; C07D231/00; C07D231/02; C07D231/10; C07D231/14; C07D231/44; C07D231/46; C07D231/48; G01N31/00; G01N31/22; G01N31/228
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
91 words
A method for determining lipoperoxides in a fluid sample obtained from a human being or other animal comprising: (a) extracting a lipoperoxide from a sample of serum, blood plasma or blood with a solvent selected from the group consisting of a hydrocarbon, an alcohol, a halogenated hydrocarbo dimethylformamide, dimethylsulfoxide and N-methyl-pyrrolidone; (b) contacting the extracted lipoperoxides with an oxidizable color reagent in the presence of a metallic compound produced from a metal which is able to have two or more valences to produce color; and (c) measuring the degree of coloring.
Claim 2
Independent Claim
91 words
A method for determining lipoperoxides in a fluid sample obtained from a human being or other animal comprising: (a) extracting a lipoperoxide from a sample of serum, blood plasma or blood with a solvent selected from the group consisting of a hydrocarbon, an alcohol, a halogenated hydrocarbo dimethylformamide, dimethylsulfoxide and N-methyl-pyrrolidone; (b) contacting the extracted lipoperoxides with an oxidizable color reagent in the presence of a metallic compound produced from a metal which is able to have two or more valences to produce color; and (c) measuring the degree of coloring.
Claim 3
Dependent Claim
18 words
A method according to claim 1, wherein the oxidizable color reagent is a phenylenediamine derivative or aniline derivative.
Claim 4
Dependent Claim
22 words
A method according to claim 1, wherein the oxidizable color reagent is a mixture of 4-aminoantipyrine or 3-alkyl-2-benzothiazolinonehydrazone with an aniline derivative.
Claim 5
Dependent Claim
85 words
A method according to claim 1, wherein the oxidizable color reagent is a reaction product of 4-aminoantipyrine and an aniline derivative having the formula: ##STR11## wherein R" 1 , and R" 2 are independently hydrogen, alkyl having 1 to 3 carbon atoms, hydroxyalkyl having 1 to 3 carbon atoms, or phenyl; and R" 3 is hydrogen, alkyl having 1 to 3 carbon atoms, sulfo, or a group of the formula X--OSO 2 -- in which X is an alkali metal, alkaline earth metal or ammonium.
Claim 6
Dependent Claim
14 words
A method according to claim 1, wherein the solvent is methanol, ethanol or propanol.
Claim 7
Dependent Claim
17 words
A method according to claim 1, wherein the metal producing the metallic compound is a transition metal.
Claim 8
Dependent Claim
15 words
A method according to claim 6, wherein the transition metal is iron, copper or vanadium.
Claim 9
Dependent Claim
20 words
A method according to claim 6, wherein the transition metal is iron, copper, manganese, cerium, cobalt, molybdenum, tungsten, or vanadium.
References Cited (Backward Citations)
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| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1984-10-19 | 1985-04-20 | 1985-10-20 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-10-19 | 1989-04-20 | 1989-10-20 | $3760.00 | Window Closed / Lapsed |
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