Patent US4332897A successfully refreshed from Google Patents.
US4332897A
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Novel bacteriophage and method for preparing same
Assignee: Noda Institute for Scientific Research
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Filing: 1978-04-12
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Granted: 1982-06-01
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Expires: 1998-04-12
Abstract
Abstract A novel bacteriophage whose DNA molecule has endonuclease-sensitivity only in the DNA region carrying genetic information for the production of phage coat proteins can be obtained by isolating an endonuclease-resistant mutant from one of the lambdoid bacteriophages and mating the resulting bacteriophage with a lambdoid phage having endonuclease-sensitivity in the DNA region carrying genetic information for the production of coat proteins.
Inventors
- 👤 Eiichi Nakano
- 👤 Narimasa Saito
- 👤 Danji Fukushima
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 19:42
CPC Classifications
C; C12; C12N; C12N15/00
Claims Analysis
Total Claims: 22
Claim 1
Independent Claim
21 words
A temperate-bacteriophage whose DNA molecule is endonuclease-sensitive only in the DNA region carrying genetic information for the production of coat proteins.
Claim 2
Dependent Claim
21 words
A temperate-bacteriophage whose DNA molecule is endonuclease-sensitive only in the DNA region carrying genetic information for the production of coat proteins.
Claim 3
Dependent Claim
14 words
A temperate-bacteriophage according to claim 1, wherein the temperate phage is a lambdoid phage.
Claim 4
Dependent Claim
22 words
A temperate-bacteriophage according to claim 2, wherein the lambdoid phage is λ(IFO 20016), 434(IFO 20018), 82(IFO 200l9), φ80(IFO 20020), or φ170(IFO 20021).
Claim 5
Dependent Claim
17 words
A temperate-bacteriophage according to any of claims 1 to 2, wherein the endonuclease is a restriction enzyme.
Claim 6
Dependent Claim
18 words
A temperate-bacteriophage according to claim 4, wherein the restriction enzyme is Eco RI, Bam HI or Hind III.
Claim 7
Dependent Claim
6 words
Bacteriophage λcI857RI r h80 (ATCC 31285).
Claim 8
Dependent Claim
6 words
Bacteriophage λcI857RI r h80 (ATCC 31285).
Claim 9
Dependent Claim
30 words
A culture of E. coli K12 strain lysogenized with the bacteriophage whose DNA molecule is endonuclease-sensitive only in the DNA region carrying genetic information for the production of coat proteins.
Claim 10
Dependent Claim
30 words
A culture of E. coli K12 strain lysogenized with the bacteriophage whose DNA molecule is endonuclease-sensitive only in the DNA region carrying genetic information for the production of coat proteins.
Claim 11
Dependent Claim
16 words
A cultue of E. coli K12 strain lysogenized with the bacteriophage λcI857RI r h80 (ATCC 31285).
Claim 12
Dependent Claim
16 words
A cultue of E. coli K12 strain lysogenized with the bacteriophage λcI857RI r h80 (ATCC 31285).
Claim 13
Independent Claim
62 words
A method for preparing a novel bacteriophage, which comprises making a bacteriophage of the lambdoid phage species endonuclease-resistant and mating the resulting bacteriophage with a lambdoid phage having endonuclease-sensitivity in the DNA region carrying genetic information for the production of coat proteins to obtain a bacteriophage having endonuclease-sensitivity only in the DNA region carrying genetic information for the production of coat proteins.
Claim 14
Independent Claim
62 words
A method for preparing a novel bacteriophage, which comprises making a bacteriophage of the lambdoid phage species endonuclease-resistant and mating the resulting bacteriophage with a lambdoid phage having endonuclease-sensitivity in the DNA region carrying genetic information for the production of coat proteins to obtain a bacteriophage having endonuclease-sensitivity only in the DNA region carrying genetic information for the production of coat proteins.
Claim 15
Dependent Claim
26 words
A method according to claim 9, wherein the bacteriophage of the lambdoid phage species is λ(IFO 20016), 434(IFO 20018), 82(IFO 20019), φ80(IFO 20020) or φ170(IFO 20021).
Claim 16
Independent Claim
18 words
A method according to any one of claims 9 and 10, wherein the endonuclease is a restriction enzyme.
Claim 17
Dependent Claim
18 words
A method according to claim 11, wherein the restriction enzyme is Eco RI, Bam HI, or Hind III.
Claim 18
Dependent Claim
21 words
A method according to claim 9, wherein the endonuclease-resistant bacteriophage of the lambdoid phage species is made by microbial concentration technique.
Claim 19
Dependent Claim
36 words
A method according to claim 9, wherein the endonuclease-resistant bacteriophage of the lambdoid phage species is made by locally deleting the region of DNA cleavable by the restriction enzyme and then using the microbial concentration technique.
Claim 20
Dependent Claim
39 words
A method according to claim 14, wherein the local deletion of the region of DNA cleavable by the restriction enzyme consists in isolating a deletion mutant in which the phage DNA region including the cleavage sites has been deleted.
Claim 21
Dependent Claim
41 words
A method according to claim 9, wherein the mating is carried out by allowing a phage resistant to the restriction enzyme and a phage sensitive to the restriction enzyme successively or simultaneously to infect a Escherichia coli sensitive to both phages.
Claim 22
Dependent Claim
38 words
A method according to claim 9, wherein the prepared novel phage is isolated by growing the novel phage in the E. coli strain which can not adsorb the endonuclease-resistant bacteriophage and in which the endonuclease-sensitive phage is lysogenized.
References Cited (Backward Citations)
US4259444A
Cited
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| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1985-05-31 | 1985-11-30 | 1986-06-01 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1989-05-31 | 1989-11-30 | 1990-06-01 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1993-05-31 | 1993-11-30 | 1994-06-01 | $7700.00 | Window Closed / Lapsed |