US4339427A
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Radioimmunoassay of thymosinα
Assignee: George Washington University
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Filing: 1980-04-14
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Granted: 1982-07-13
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Expires: 2000-04-14
Abstract
Abstract An improved radioimmunoassay for the polypeptidic thymic hormone thymosin alpha 1 is described. The assay employs a radiolabelled [Tyr1]-thymosin alpha 1 or [Tyr1]-desacetylthymosin alpha 1 as probe and an affinity purified thymosin alpha 1 antibody of improved selectivity.
Inventors
- 👤 Allan L. Goldstein
- 👤 John E. McClure
- 👤 Su-Sun Wang
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 08:55
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/74; Y; Y10; Y10S; Y10S530/00; Y10S530/827; Y10S530/834; Y10S530/835; Y10S530/837; Y10S930/00; Y10S930/01; Y10S930/18
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
6 words
[Tyr 1 ]-thymosin α 1 .
Claim 2
Dependent Claim
6 words
[Tyr 1 ]-thymosin α 1 .
Claim 3
Dependent Claim
6 words
[Tyr 1 ]-desacetylthymosin α 1 .
Claim 4
Dependent Claim
6 words
[Tyr 1 ]-desacetylthymosin α 1 .
Claim 5
Dependent Claim
7 words
125 I-[Tyr 1 ]-Thymosin α 1 .
Claim 6
Dependent Claim
7 words
125 I-[Tyr 1 ]-Thymosin α 1 .
Claim 7
Dependent Claim
7 words
125 I-[Tyr 1 ]-desacetylthymosin α 1 .
Claim 8
Dependent Claim
7 words
125 I-[Tyr 1 ]-desacetylthymosin α 1 .
Claim 9
Dependent Claim
119 words
In a method for the assay of thymosin α 1 in a sample, which method comprises mixing said sample with a known amount of labelled thymosin α 1 and an antibody which will selectively complex with said thymosin α 1 , separating the resulting antibody-antigen complex from uncomplexed labelled thymosin α 1 , measuring the degree of binding of the said labelled thymosin α 1 in said complex and determining the amount of thymosin α 1 present in said sample by comparing said degree of binding to a standard curve, the improvement which comprises utilizing as the labelled thymosin α 1 a compound selected from 125 I-[Tyr 1 ]-thymosin α 1 and 125 I-[Tyr 1 ]-desacetylthymosin α 1 .
Claim 10
Dependent Claim
119 words
In a method for the assay of thymosin α 1 in a sample, which method comprises mixing said sample with a known amount of labelled thymosin α 1 and an antibody which will selectively complex with said thymosin α 1 , separating the resulting antibody-antigen complex from uncomplexed labelled thymosin α 1 , measuring the degree of binding of the said labelled thymosin α 1 in said complex and determining the amount of thymosin α 1 present in said sample by comparing said degree of binding to a standard curve, the improvement which comprises utilizing as the labelled thymosin α 1 a compound selected from 125 I-[Tyr 1 ]-thymosin α 1 and 125 I-[Tyr 1 ]-desacetylthymosin α 1 .
Claim 11
Dependent Claim
18 words
The improved method of claim 5 wherein said labelled compound is 125 I-[Tyr 1 ]-thymosin α 1 .
Claim 12
Dependent Claim
40 words
The improved method of claim 5 wherein said antibody comprises anantibody pretreated with a fraction 5 preparation obtained from a mammalian organ not containing thymosin α 1 producing cells so as to obtain an antibody of enhanced sensitivity and specificity.
Claim 13
Dependent Claim
13 words
The improved method of claim 7 wherein said mammalian organ is bovine kidney.
Claim 14
Dependent Claim
127 words
In a method for the assay of thymosin α 1 in a sample, which method comprises mixing said sample with a known amount of labelled thymosin α 1 and an antibody which will selectively complex with said thymosin α 1 , separating the resulting antibody-antigen complex from uncomplexed labelled thymosin α 1 , measuring the degree of binding of the said labelled thymosin α 1 in said complex and determining the amount of thymosin α 1 present in said sample by comparing said degree of binding to a standard curve, the improvement which comprises utilizing as said antibody an antibody pretreated with a fraction 5 preparation obtained from a mammalian organ not containing thymosin α 1 so as to obtain an antibody of enhanced sensitivity and specificity.
Claim 15
Dependent Claim
127 words
In a method for the assay of thymosin α 1 in a sample, which method comprises mixing said sample with a known amount of labelled thymosin α 1 and an antibody which will selectively complex with said thymosin α 1 , separating the resulting antibody-antigen complex from uncomplexed labelled thymosin α 1 , measuring the degree of binding of the said labelled thymosin α 1 in said complex and determining the amount of thymosin α 1 present in said sample by comparing said degree of binding to a standard curve, the improvement which comprises utilizing as said antibody an antibody pretreated with a fraction 5 preparation obtained from a mammalian organ not containing thymosin α 1 so as to obtain an antibody of enhanced sensitivity and specificity.
Claim 16
Dependent Claim
18 words
The improved method of claim 9 wherein said labelled compound is 125 I-[Tyr 1 ]-thymosin α 1 .
References Cited (Backward Citations)
US4264571A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1985-07-12 | 1986-01-11 | 1986-07-13 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1989-07-12 | 1990-01-11 | 1990-07-13 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1993-07-12 | 1994-01-11 | 1994-07-13 | $7700.00 | Window Closed / Lapsed |