Patent US4348479A successfully refreshed from Google Patents.
US4348479A
Expired / Lapsed
US
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Recovery of proteinaceous material having reduced nucleic acid levels
Assignee: Cornell Research Foundation Inc
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Filing: 1980-08-18
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Granted: 1982-09-07
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Expires: 2000-08-18
Abstract
Abstract The present invention relates to a process which comprises recovering proteinaceous materials from nucleoprotein complexes by derivatizing the proteinaceous material with a cyclic anhydride, thereby disassocating the complex, separating the derivatized proteinaceous material from the nucleic acids and subsequently regenerating the proteinaceous material. The recovery of the proteinaceous material is accomplished by maintaining the separated derivatized proteinaceous material at a suitable acidic pH, which is a function of the reaction time and the temperature, until the derivatized proteinaceous material is disassociated into the proteinaceous material and the cyclic anhydride or most usually the equivalent acid and the proteinaceous material is recovered.
Inventors
- 👤 John E. Kinsella
- 👤 Jayarama K. Shetty
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 16:18
CPC Classifications
A; A23; A23J; A23J1/00; A23J1/18; A23J1/008; Y; Y10; Y10S; Y10S435/00; Y10S435/814; Y10S530/00; Y10S530/82; Y10S530/821
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
125 words
A process of recovering microbially derived proteinaceous material essentially devoid of nucleic acid from nucleoprotein complexes which comprises: (a) disrupting microbial cells to provide a mixture comprising proteinaceous material and nucleic acid; (b) treating an aqueous mixture of proteinaceous material and nucleic acid with an organic dicarboxylic anhydride containing a beta-diene structure at a pH of above about 7.5 thereby derivatizing the proteinaceous material; (c) reducing the pH to isoelectrically precipitate the derivatized proteinaceous material and recovering the derivatized proteinaceous material which is thereby separated from nucleic acid; (d) maintaining the recovered derivatized proteinaceous material at an acidic pH until the derivatized proteinaceous material disassociates into the proteinaceous material and the organic dicarboxylic anhydrides or a derivative thereof, and separating and recovering the proteinaceous material.
Claim 2
Dependent Claim
125 words
A process of recovering microbially derived proteinaceous material essentially devoid of nucleic acid from nucleoprotein complexes which comprises: (a) disrupting microbial cells to provide a mixture comprising proteinaceous material and nucleic acid; (b) treating an aqueous mixture of proteinaceous material and nucleic acid with an organic dicarboxylic anhydride containing a beta-diene structure at a pH of above about 7.5 thereby derivatizing the proteinaceous material; (c) reducing the pH to isoelectrically precipitate the derivatized proteinaceous material and recovering the derivatized proteinaceous material which is thereby separated from nucleic acid; (d) maintaining the recovered derivatized proteinaceous material at an acidic pH until the derivatized proteinaceous material disassociates into the proteinaceous material and the organic dicarboxylic anhydrides or a derivative thereof, and separating and recovering the proteinaceous material.
Claim 3
Dependent Claim
13 words
A process as in claim 1 where the proteinaceous material is an enzyme.
Claim 4
Dependent Claim
23 words
A method as in claim 1 or 2 where the organic dicarboxylic acid anhydride is citraconic anhydride, maleic anhydride or dimethyl maleic anhydride.
Claim 5
Dependent Claim
14 words
A method as in claim 1 or 2 where the anhydride is citraconic anhydride.
Claim 6
Dependent Claim
24 words
A method as in claim 1 where the isoelectric precipitation of the derivatized proteinaceous material is conducted at a pH of 4.2 to 4.5.
Claim 7
Dependent Claim
22 words
A method as in claim 1 where the recovered derivatized proteinaceous material is maintained at an acidic pH of below about 5.0.
Claim 8
Dependent Claim
14 words
A method as in claim 6 where the acidic pH is 3.5 to 4.5.
Claim 9
Dependent Claim
14 words
A method as in claim 6 where the acidic pH is 4.0 to 4.3.
References Cited (Backward Citations)
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1985-09-06 | 1986-03-08 | 1986-09-07 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1989-09-06 | 1990-03-08 | 1990-09-07 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1993-09-06 | 1994-03-08 | 1994-09-07 | $7700.00 | Window Closed / Lapsed |