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US4351823A
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Diagnosis of tumors or bacterial infections having β-glucuronidase activity
Assignee: CENTURY SCIENCE CORP 99 SOUNDVIEW DRIVE PORT WASHINGTON NEW YORK 11050 A CORP
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Filing: 1980-07-18
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Granted: 1982-09-28
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Expires: 2000-07-18
Abstract
Abstract A method of diagnosing the presence of tumors having β-glucuronidase activity comprises administering a glucuronide which differs in color or color intensity from the aglycone thereof to a patient. The preferred glucuronide is phenolphthalein glucuronide. The urine is then collecting for a twenty four hour period and the amount of phenolphthalein glucuronides present in the urine is determined. If substantially all of the phenolphthalein glucuronide is expelled then it is apparent that the body has no tumors or bacterial infections exhibiting β-glucuronidase activity. If however substantial amounts of phenolphthalein is retained, then this is an indication of the presence of tumors having β-glucuronidase activity or a bacterial infection having β-glucuronidase activity.
Inventors
- 👤 David Rubin
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 23:37
CPC Classifications
A; A61; A61K; A61K51/00; A61K51/02; A61K51/04; A61K51/0491; A61K31/00; A61K31/70; A61K31/7004; A61K47/00; A61K47/50; A61K47/51; A61K47/54; A61K47/549; C; C07; C07H; C07H15/00; C07H15/18; C07H15/20; C07H15/203; C12; C12Q; C12Q1/00; C12Q1/34; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/5005; G01N33/5091; A61K2123/00; C12Q2334/00; C12Q2334/40; G01N2333/00; G01N2333/90; G01N2333/914; G01N2333/924
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
151 words
A method for diagnosing the presence of tumors or bacterial infections having β-glucuronidase activity, in a patient, comprising: injecting a non-toxic conjugated glucuronide of an aglycone, which aglycone, in the free state thereof, differs in color, color intensity, or fluorescence from the conjugated glucuronide thereof; collecting the urine of the patient for a period of time sufficient to permit most of the injected glcuronide to be expelled from the body if it is not retained therein; and determining the amount of glucuronide present in the collected urine by deconjugating the glucuronide in a sample of the urine and comparing the color, color intensity or fluorescence of the aglycone with a standard curve, thus indicating the quantity of aglycone in the sample; whereby, if it appears that a substantial amount of glucuronide has been retained in the body, this is a positive indication of a tumor or bacterial infection having β-glucuronidase activity.
Claim 2
Independent Claim
151 words
A method for diagnosing the presence of tumors or bacterial infections having β-glucuronidase activity, in a patient, comprising: injecting a non-toxic conjugated glucuronide of an aglycone, which aglycone, in the free state thereof, differs in color, color intensity, or fluorescence from the conjugated glucuronide thereof; collecting the urine of the patient for a period of time sufficient to permit most of the injected glcuronide to be expelled from the body if it is not retained therein; and determining the amount of glucuronide present in the collected urine by deconjugating the glucuronide in a sample of the urine and comparing the color, color intensity or fluorescence of the aglycone with a standard curve, thus indicating the quantity of aglycone in the sample; whereby, if it appears that a substantial amount of glucuronide has been retained in the body, this is a positive indication of a tumor or bacterial infection having β-glucuronidase activity.
Claim 3
Dependent Claim
13 words
A method in accordance with claim 1, wherein said glucuronide is phenolphthalein glucuronide.
Claim 4
Independent Claim
21 words
A method in accordance with claims 1 or 2, wherein the urine is collected for a period of twenty four hours.
Claim 5
Dependent Claim
42 words
A method in accordance with claim 2, wherein the urine is analyzed by taking a sample of the urine, deconjugating the phenolphthalein glucuronide, alkalinizing and comparing the color intensity with a standard curve, thus indicating the quantity of phenolphthalein in the sample.
Claim 6
Dependent Claim
19 words
A method in accordance with claim 4, wherein said glucuronide is deconjugated by treating with a concentrated mineral acid.
Claim 7
Dependent Claim
31 words
A method in accordance with claim 1, wherein prior to or concurrent with administration of the glucuronide a hyperacidifying agent is added to hyperacidify any tumor cells which may be present.
Claim 8
Dependent Claim
48 words
A process in accordance with claim 1 or claim 6, wherein previous to or concurrent with administration of the glucuronide an alkalinizing agent is added in an amount sufficient to maintain the pH level of the non-tumor tissues of the patient at approximately 7.4 during the glucuronide treatment.
Claim 9
Dependent Claim
15 words
A process in accordance with claim 6, wherein said hyperacidifying agent comprises a hyperglycemic agent.
Claim 10
Dependent Claim
15 words
A process in accordance with claim 7, wherein said hyperacidifying agent comprises a hyperglycemic agent.
Claim 11
Dependent Claim
27 words
A process in accordance with claim 8 or claim 9, wherein the hyperglycemic agent is selected from the group consisting of glucose, fructose, galactose, lactose and glucogan.
Claim 12
Dependent Claim
18 words
A process in accordance with claim 7, wherein said alkalinizing agent comprises an alkali metal bicarbonate or citrate.
References Cited (Backward Citations)
US4327074A
Cited
US4337760A
Cited
USPTO Annuity & Maintenance Windows
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| 3.5 Years (Year 4) | 1985-09-27 | 1986-03-29 | 1986-09-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1989-09-27 | 1990-03-29 | 1990-09-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1993-09-27 | 1994-03-29 | 1994-09-28 | $7700.00 | Window Closed / Lapsed |