US4379843A
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Immobilization of polynucleotides and polypeptides with tritylated polysaccharides
Assignee: Individual
•
Filing: 1981-01-26
•
Granted: 1983-04-12
•
Expires: 2001-01-26
Abstract
Abstract Polynucleotides and Polypeptides are immobilized and/or isolated by using a triphenylmethyl ether derivative of polysaccharides in hydrated form.
Inventors
- 👤 Peter Cashion
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 08:46
CPC Classifications
C; C07; C07H; C07H21/00; C08; C08B; C08B37/00; C08B37/0006; C08B37/0036; C08B37/0039; C12; C12N; C12N11/00; C12N11/02; C12N11/10; C12N9/00; C12N9/14; C12N9/16; C12N9/22; C12P; C12P19/00; C12P19/26; C12P19/28; C12P19/30; C12P19/34; C12P21/00; C12P21/02; Y; Y10; Y10S; Y10S435/00; Y10S435/814; Y10S435/815
Claims Analysis
Total Claims: 49
Claim 1
Independent Claim
24 words
A composition of matter, comprising a polynucleotide or polypeptide attracted to a substantially hydrated polysaccharide through a triphenylmethyl ether group bonded to said polysaccharide.
Claim 2
Dependent Claim
24 words
A composition of matter, comprising a polynucleotide or polypeptide attracted to a substantially hydrated polysaccharide through a triphenylmethyl ether group bonded to said polysaccharide.
Claim 3
Dependent Claim
16 words
A composition of matter as recited in claim 1 wherein the polynucleotide is a ribonucleic acid.
Claim 4
Dependent Claim
16 words
A composition of matter as recited in claim 2 wherein the ribonucleic acid is messenger RNA.
Claim 5
Dependent Claim
16 words
A composition of matter as recited in claim 2 wherein the ribonucleic acid is ribosomal RNA.
Claim 6
Dependent Claim
16 words
A composition of matter as recited in claim 2 wherein the ribonucleic acid is transfer RNA.
Claim 7
Dependent Claim
16 words
A composition of matter as recited in claim 2 wherein the ribonucleic acid is viral RNA.
Claim 8
Dependent Claim
16 words
A composition of matter as recited in claim 1 wherein the polynucleotide is a deoxyribonucleic acid.
Claim 9
Dependent Claim
17 words
A composition of matter as recited in claim 7 wherein the deoxyribonucleic acid includes a denatured DNA.
Claim 10
Dependent Claim
26 words
A composition of matter comprising a poly A containing ribonucleic acid attracted to a substantially hydrated polysaccharide through a triphenylmethyl ether group bonded to said polysaccharide.
Claim 11
Dependent Claim
26 words
A composition of matter comprising a poly A containing ribonucleic acid attracted to a substantially hydrated polysaccharide through a triphenylmethyl ether group bonded to said polysaccharide.
Claim 12
Dependent Claim
33 words
A composition of matter, comprising a polypeptide attracted to a substantially hydrated polysaccharide through a triphenylmethyl ether group bonded to said polysaccharide, said polysaccharide being hydrated at least about 60 percent by weight.
Claim 13
Dependent Claim
33 words
A composition of matter, comprising a polypeptide attracted to a substantially hydrated polysaccharide through a triphenylmethyl ether group bonded to said polysaccharide, said polysaccharide being hydrated at least about 60 percent by weight.
Claim 14
Dependent Claim
15 words
A composition of matter as recited in claim 10 wherein the polypeptide is an enzyme.
Claim 15
Dependent Claim
15 words
A composition of matter as recited in claim 11 wherein the enzyme is a nuclease.
Claim 16
Dependent Claim
15 words
A composition of matter as recited in claim 11 wherein the enzyme is a protease.
Claim 17
Dependent Claim
15 words
A composition of matter as recited in claim 11 wherein the enzyme is an endonuclease.
Claim 18
Dependent Claim
39 words
A composition of matter as recited in claim 11 wherein the enzyme is a member selected from the group consisting of polynucleotide phosphorylase, DNA polymerase, RNA polymerase, RNA ligase, polynucleotide kinase, AMV reverse transcriptase, and phospho glyceric acid kinase.
Claim 19
Dependent Claim
45 words
A composition of matter, comprising a polynucleotide or polypeptide attracted to a substantially hydrated polysaccharide through a triphenylmethyl ether group bonded to said polysaccharide, said polysaccharide being a member selected from the group consisting of agarose, cellulose, dextran, glycogen, carragheenan, starch, or a mixture thereof.
Claim 20
Dependent Claim
45 words
A composition of matter, comprising a polynucleotide or polypeptide attracted to a substantially hydrated polysaccharide through a triphenylmethyl ether group bonded to said polysaccharide, said polysaccharide being a member selected from the group consisting of agarose, cellulose, dextran, glycogen, carragheenan, starch, or a mixture thereof.
Claim 21
Dependent Claim
21 words
A composition of matter as recited in claim 16 wherein the hydrated polysaccharide contains at least about 60% water by weight.
Claim 22
Dependent Claim
21 words
A composition of matter as recited in claim 16 wherein the hydrated polysaccharide contains at least about 80% water by weight.
Claim 23
Dependent Claim
21 words
A composition of matter as recited in claim 16 wherein the hydrated polysaccharide contains at least about 95% water by weight.
Claim 24
Dependent Claim
44 words
A process for the immobilization of a polynucleotide or polypeptide, comprising forming a reaction product of triphenylmethyl ether of polysaccharide, hydrating substantially said reaction product, and reacting said hydrated reaction product with said polynucleotide or polypeptide to produce an immobilized composition of matter thereof.
Claim 25
Dependent Claim
44 words
A process for the immobilization of a polynucleotide or polypeptide, comprising forming a reaction product of triphenylmethyl ether of polysaccharide, hydrating substantially said reaction product, and reacting said hydrated reaction product with said polynucleotide or polypeptide to produce an immobilized composition of matter thereof.
Claim 26
Dependent Claim
12 words
A process of claim 20 wherein the polynucleotide is a ribonucleic acid.
Claim 27
Dependent Claim
12 words
A process of claim 21 wherein the ribonucleic acid is messenger RNA.
Claim 28
Dependent Claim
12 words
A process of claim 21 wherein the ribonucleic acid is ribosomal RNA.
Claim 29
Dependent Claim
13 words
A process of claim 21 wherein the ribonucleic acid is a transfer RNA.
Claim 30
Dependent Claim
13 words
A process of claim 21 wherein the ribonucleic acid is a viral RNA.
Claim 31
Dependent Claim
14 words
A process as recited in claim 20 wherein the polynucleotide is a deoxyribonucleic acid.
Claim 32
Dependent Claim
15 words
A process as recited in claim 26 wherein the deoxyribonucleic acid includes a denatured DNA.
Claim 33
Dependent Claim
44 words
A process for the immobilization of a poly A containing ribonucleic acid, comprising forming a reaction product of triphenylmethyl ether of polysaccharide, hydrating substantially said reaction product, and reacting said hydrated reaction product with a poly A containing ribonucleic acid to immobilize said acid.
Claim 34
Dependent Claim
44 words
A process for the immobilization of a poly A containing ribonucleic acid, comprising forming a reaction product of triphenylmethyl ether of polysaccharide, hydrating substantially said reaction product, and reacting said hydrated reaction product with a poly A containing ribonucleic acid to immobilize said acid.
Claim 35
Dependent Claim
33 words
A process for immobilizing a polypeptide, comprising forming a reaction product of triphenylmethyl ether of polysaccharide, hydrating substantially said reaction product, and reacting said hydrated reaction product with polypeptide to immobilize the same.
Claim 36
Dependent Claim
33 words
A process for immobilizing a polypeptide, comprising forming a reaction product of triphenylmethyl ether of polysaccharide, hydrating substantially said reaction product, and reacting said hydrated reaction product with polypeptide to immobilize the same.
Claim 37
Dependent Claim
11 words
A process of claim 29 wherein the polypeptide is an enzyme.
Claim 38
Dependent Claim
11 words
A process of claim 30 wherein the enzyme is a nuclease.
Claim 39
Dependent Claim
11 words
A process of claim 30 wherein the enzyme is a protease.
Claim 40
Dependent Claim
11 words
A process of claim 30 wherein the enzyme is an endonuclease.
Claim 41
Dependent Claim
10 words
A process of claim 29 wherein the polypeptide is interferon.
Claim 42
Dependent Claim
37 words
A process as recited in claim 29 wherein the polypeptide is an enzyme selected from the group consisting of polynucleotide phosphorylase, DNA polymerase, RNA polymerase, RNA ligase, polynucleotide kinase, AMV reverse transcriptase, and phospho glyceric acid kinase.
Claim 43
Dependent Claim
61 words
A process for immobilization of a polynucleotide or polypeptide, comprising forming a reaction product of triphenylmethyl ether of polysaccharide, said polysaccharide being a member selected from the group consisting of agarose, cellulose, dextran, glycogen, carragheenan, starch, or a mixture thereof, hydrating substantially said reaction product, reacting said product with a polynucleotide or polypeptide to produce an immobilized composition of matter thereof.
Claim 44
Dependent Claim
61 words
A process for immobilization of a polynucleotide or polypeptide, comprising forming a reaction product of triphenylmethyl ether of polysaccharide, said polysaccharide being a member selected from the group consisting of agarose, cellulose, dextran, glycogen, carragheenan, starch, or a mixture thereof, hydrating substantially said reaction product, reacting said product with a polynucleotide or polypeptide to produce an immobilized composition of matter thereof.
Claim 45
Dependent Claim
28 words
A process of isolating a polynucleotide or polypeptide, comprising contacting a composition including said polynucleotide or polypeptide with a substantially hydrated gel of triphenylmethyl ether of a polysaccharide.
Claim 46
Dependent Claim
28 words
A process of isolating a polynucleotide or polypeptide, comprising contacting a composition including said polynucleotide or polypeptide with a substantially hydrated gel of triphenylmethyl ether of a polysaccharide.
Claim 47
Dependent Claim
27 words
A process of claim 37 wherein the polysaccharide is agarose and the hydrated gel of triphenylmethyl ether of agarose contains at least about 60 weight percent water.
Claim 48
Dependent Claim
20 words
A process of claim 37 wherein the triphenylmethyl ether of the polysaccharide is hydrated at least about 80 weight percent.
Claim 49
Dependent Claim
20 words
A process of claim 37 wherein the triphenylmethyl ether of the polysaccharide is hydrated at least about 95 weight percent.
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| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1986-04-11 | 1986-10-11 | 1987-04-12 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-04-11 | 1990-10-11 | 1991-04-12 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-04-11 | 1994-10-11 | 1995-04-12 | $7700.00 | Window Closed / Lapsed |