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US4388295A
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Method and composition for determining human chorionic somatotropin
Assignee: Istituto Sieroterapico e Vaccinogeno Toscano Sclavo SpA
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Filing: 1980-05-30
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Granted: 1983-06-14
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Expires: 2000-05-30
Abstract
Abstract A method and an implementation are disclosed for measuring the contents of a certain component (e.g. a hormone) in a biological fluid. The basic principle of the method is that a complex is formed starting from the antagonist of the component sought for with the product of conjugation of the latter with a tracer (e.g. a radioisotope), said complex is introduced in the biological fluid to be tested and the quantity of the tracer which has been set free is measured with a measuring or dosing technique which is appropriate for the tracer which has been selected.
Inventors
- 👤 Francesco Cocola
- 👤 Paolo Tarli
- 👤 Paolo Neri
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 23:32
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/58; G01N2470/00; G01N2470/10; G01N2470/12; Y; Y10; Y10S; Y10S435/00; Y10S435/967; Y10S435/97; Y10S435/975; Y10T; Y10T436/00; Y10T436/13
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
63 words
A method for quantitatively determining the presence of Human Chorionic Somatotropin (HCS) in a biological fluid comprising: (a) forming a complex consisting essentially of an antagonist of HCS with the conjugation product of HCS and a tracer; (b) introducing said complex into a sample of biological fluid suspected of containing HCS; and (c) measuring the quantity of tracer which has been set free.
Claim 2
Independent Claim
63 words
A method for quantitatively determining the presence of Human Chorionic Somatotropin (HCS) in a biological fluid comprising: (a) forming a complex consisting essentially of an antagonist of HCS with the conjugation product of HCS and a tracer; (b) introducing said complex into a sample of biological fluid suspected of containing HCS; and (c) measuring the quantity of tracer which has been set free.
Claim 3
Dependent Claim
23 words
The method of claim 1 further comprising fixing the complex to a solid supporting member prior to introducing said complex into said sample.
Claim 4
Dependent Claim
60 words
The method of claim 1 wherein the solid supporting member is selected from the group consisting of cellulose in powder or paper form, cellulose having particular reactive groups as carboxymethyl or diethylaminoethyl in powder or paper form, polyvinyl chloride, polystyrene, nylon, polymethacrylate, polyamide, ureic resins, cellulose acetate, cellulose triacetate, cellulose nitrate, cellulose esters and ethers and derivatives thereof, and glass.
Claim 5
Dependent Claim
29 words
The method of claim 1 wherein the tracer is selected from the group consisting of radioisotopes, fluorescent substances, dyestuffs, enzymes, inhibitors of enzymatic and non-enzymatic reactions and complex-forming agents.
Claim 6
Dependent Claim
40 words
A composition adapted to quantitatively determine the presence of Human Chorionic Somatotropin (HCS) in a biological fluid which comprises a complex of an antagonist of HCS with the conjugation product of HCS and a tracer on a solid supporting member.
Claim 7
Dependent Claim
40 words
A composition adapted to quantitatively determine the presence of Human Chorionic Somatotropin (HCS) in a biological fluid which comprises a complex of an antagonist of HCS with the conjugation product of HCS and a tracer on a solid supporting member.
Claim 8
Dependent Claim
28 words
The composition of claim 5 wherein said tracer is selected from the group consisting of radioisotopes, fluorescent substances, dyestuffs, inhibitors of enzymatic and non-enzymatic reactions, and complex-forming agents.
Claim 9
Dependent Claim
38 words
A merchandising unit comprising containers of substances adapted to quantitatively determine the presence of HCS in a biological fluid formed by the composition of claim 5 and a set of reference standards of HCS having a known concentration.
Claim 10
Dependent Claim
62 words
The merchandising unit of claim 7 wherein the solid supporting member is selected from the group consisting of cellulose in powder or paper form, cellulose having particular reactive groups such as carboxymethyl or diethylaminoethyl in powder or paper form, polyvinyl chloride, polystyrene, nylon, polymethacrylate, polyamide, ureic resins, cellulose acetate, cellulose triacetate, cellulose nitrate, cellulose esters and ethers and derivatives thereof, and glass.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-06-13 | 1986-12-13 | 1987-06-14 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-06-13 | 1990-12-13 | 1991-06-14 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-06-13 | 1994-12-13 | 1995-06-14 | $7700.00 | Window Closed / Lapsed |