US4397840A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Novel erythropoietin product and method for the preparation thereof
Assignee: Ajinomoto Co Inc
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Filing: 1982-03-03
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Granted: 1983-08-09
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Expires: 2002-03-03
Abstract
Abstract The invention provides a novel erythropoietin product prepared from the urine of healthy human but exhibiting no inhibitory effect against erythropoiesis as is the most disadvantageous problem in the product prepared from the urine of healthy human. The method for the preparation of such a product comprises adsorbing the crude erythropoietin product obtained from the urine of healthy human on to a weakly basic anion exchanger from a neutral or weakly acidic aqueous solution containing 0.1 to 0.2 mole per liter of an inorganic neutral salt such as sodium chloride and then eluting the thus adsorbed ingredients with an aqueous eluant solution containing from 0.5 to 0.7 mole per liter of an inorganic neutral salt.
Inventors
- 👤 Kenji Takezawa
- 👤 Hajima Hiratani
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 10:44
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/475; C07K14/505; A; A61; A61K; A61K38/00; Y; Y10; Y10S; Y10S530/00; Y10S530/827; Y10S530/834
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
100 words
A method for the preparation of an erythropoietin product having no inhibitory effect against erythropoiesis which comprises the steps of (a) adsorbing a crude erythropoietin product obtained from the urine of healthy human on to a weakly basic anion exchanger from a neutral or weakly acidic aqueous solution in the presence of an inorganic neutral salt in a concentration in the range from 0.1 to 0.2 mole per liter, and (b) eluting the thus adsorbed erythropoietin product with an aqueous eluant solution containing an inorganic neutral salt in a concentration in the range from 0.5 to 0.7 mole per liter.
Claim 2
Independent Claim
100 words
A method for the preparation of an erythropoietin product having no inhibitory effect against erythropoiesis which comprises the steps of (a) adsorbing a crude erythropoietin product obtained from the urine of healthy human on to a weakly basic anion exchanger from a neutral or weakly acidic aqueous solution in the presence of an inorganic neutral salt in a concentration in the range from 0.1 to 0.2 mole per liter, and (b) eluting the thus adsorbed erythropoietin product with an aqueous eluant solution containing an inorganic neutral salt in a concentration in the range from 0.5 to 0.7 mole per liter.
Claim 3
Dependent Claim
34 words
The method as claimed in claim 1 wherein the inorganic neutral salt used in step (a) is selected from the group consisting of sodium chloride, potassium chloride, magnesium chloride, calcium chloride and sodium bromide.
Claim 4
Dependent Claim
17 words
The method as claimed in claim 1 wherein the weakly basic anion exchanger is a diethylaminoethylated carbohydrate.
Claim 5
Dependent Claim
25 words
The method as claimed in claim 3 wherein the diethylaminoethylated carbohydrate is selected from the group consisting of diethylaminoethyl cellulose, diethylaminoethyl dextran and diethylaminoethyl agarose.
Claim 6
Dependent Claim
25 words
The method as claimed in claim 1 wherein the neutral or weakly acidic aqueous solution has a pH in the range from 6.0 to 7.5.
Claim 7
Dependent Claim
22 words
The method as claimed in claim 1 wherein the aqueous eluant solution had a pH in the range from 6.0 to 7.5.
Claim 8
Dependent Claim
32 words
An erythropoietin product having no inhibitory effect against erythropoiesis with a molecular weight in the range from 4,000 to about 13,000 and an isoelectric point in the range from 3.1 to 3.6.
Claim 9
Dependent Claim
32 words
An erythropoietin product having no inhibitory effect against erythropoiesis with a molecular weight in the range from 4,000 to about 13,000 and an isoelectric point in the range from 3.1 to 3.6.
References Cited (Backward Citations)
US4303650A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-08-08 | 1987-02-07 | 1987-08-09 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-08-08 | 1991-02-07 | 1991-08-09 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-08-08 | 1995-02-07 | 1995-08-09 | $7700.00 | Window Closed / Lapsed |