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US4410625A
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Salt-tolerant microbial xanthanase and method of producing same
Assignee: United States Department of Agriculture
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Filing: 1982-02-04
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Granted: 1983-10-18
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Expires: 2002-02-04
Abstract
Abstract A novel Bacillus isolated from the soil is unique in its capacity to produce xanthanase, and is especially productive of the enzyme when cultured in the presence of other soil organisms. Both crude and purified xanthanases recovered from the fermentation broth effectively degrade xanthan gum. Moreover, tolerance of the Bacillus to sodium chloride levels as high as about 4%, render it useful for the in situ degradation of the heteropolysaccharide in petroleum recovery fluids and other thickened industrial brines.
Inventors
- 👤 Martin C. Cadmus
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 17:42
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/24; C12N9/2402; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/829; Y10S435/83; Y10S435/84; Y10S435/85; Y10S435/859; Y10S435/91
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
84 words
A method for degrading xanthan gum comprising fermenting said gum with a mixed culture comprising: (1) a first soil bacterium bearing the ARS Culture Collection Accession No. NRRL B-4529; and (2) at least one other soil bacterium capable of enhancing the growth of said first soil bacterium when in mixed culture therewith on a xanthan-containing medium; wherein the fermentation is conducted under aerobic conditions suitable for the growth of said first soil bacterium and for the elaboration of xanthanase by said first soil bacterium.
Claim 2
Independent Claim
84 words
A method for degrading xanthan gum comprising fermenting said gum with a mixed culture comprising: (1) a first soil bacterium bearing the ARS Culture Collection Accession No. NRRL B-4529; and (2) at least one other soil bacterium capable of enhancing the growth of said first soil bacterium when in mixed culture therewith on a xanthan-containing medium; wherein the fermentation is conducted under aerobic conditions suitable for the growth of said first soil bacterium and for the elaboration of xanthanase by said first soil bacterium.
Claim 3
Dependent Claim
26 words
A method as described in claim 1 wherein said other soil bacterium is selected from the group of Arthrobacter, Alcaligenes, Agrobacterium, Brevibacterium, Flavobacterium, Micrococcus, and Xanthomonas.
Claim 4
Dependent Claim
41 words
A method as described in claim 1 wherein said other soil bacterium is selected from the group of Arthrobacter globiformis, Arthrobacter simplex, Arthrobacter viscosus, Alcaligenes faecalis, Agrobacterium radiobacter, Brevibacterium linens, Flavobacterium aquatile, Flavobacterium sp. NRRL B-14010, Micrococcus luteus, and Xanthomonas campestris.
Claim 5
Dependent Claim
17 words
A method as described in claim 1 wherein said other soil bacterium is Flavobacterium sp. NRRL B-14010.
Claim 6
Independent Claim
100 words
A method for producing a salt-tolerant xanthanase comprising the following steps: a. culturing on a xanthan gum-containing medium a mixed culture comprising (1) a first soil bacterium bearing the ARS Culture Collection Accession No. NRRL B-4529, and (2) at least one other soil bacterium capable of enhancing the growth of said first soil bacterium when in mixed culture therewith on a xanthan-containing medium; wherein said fermentative culturing is conducted under aerobic conditions suitable for the growth of said first soil bacterium and for the elaboration of xanthanase by said first soil bacterium; and b. recovering said xanthanase from said medium.
Claim 7
Independent Claim
100 words
A method for producing a salt-tolerant xanthanase comprising the following steps: a. culturing on a xanthan gum-containing medium a mixed culture comprising (1) a first soil bacterium bearing the ARS Culture Collection Accession No. NRRL B-4529, and (2) at least one other soil bacterium capable of enhancing the growth of said first soil bacterium when in mixed culture therewith on a xanthan-containing medium; wherein said fermentative culturing is conducted under aerobic conditions suitable for the growth of said first soil bacterium and for the elaboration of xanthanase by said first soil bacterium; and b. recovering said xanthanase from said medium.
Claim 8
Dependent Claim
26 words
A method as described in claim 5 wherein said other soil bacterium is selected from the group of Arthrobacter, Alcaligenes, Agrobacterium, Brevibacterium, Flavobacterium, Micrococcus, and Xanthomonas.
Claim 9
Dependent Claim
41 words
A method as described in claim 5 wherein said other soil bacterium is selected from the group of Arthrobacter globiformis, Arthrobacter simplex, Arthrobacter viscosus, Alcaligenes faecalis, Agrobacterium radiobacter, Brevibacterium linens, Flavobacterium aquatile, Flavobacterium sp. NRRL B-14010, Micrococcus luteus, and Xanthomonas campestris.
Claim 10
Dependent Claim
17 words
A method as described in claim 5 wherein said other soil bacterium is Flavobacterium sp. NRRL B-14010.
References Cited (Backward Citations)
US2742398A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-10-17 | 1987-04-18 | 1987-10-18 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-10-17 | 1991-04-18 | 1991-10-18 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-10-17 | 1995-04-18 | 1995-10-18 | $7700.00 | Window Closed / Lapsed |