US4446232A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Enzyme immunoassay with two-zoned device having bound antigens
Assignee: Individual
•
Filing: 1981-10-13
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Granted: 1984-05-01
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Expires: 2001-10-13
Abstract
Abstract A device for determining the presence of antigens which comprises a first zone containing antigens and enzyme-linked antibodies which are capable of immunologically reacting with said antigens, said antibodies being positioned in said first zone such that they will be removed from said first zone when reacted with antigens passing through said first zone but not removed from said first zone in the absence of such antigens, and a second zone containing material capable of reacting with said enzyme-linked antibodies to produce a color forming reaction which indicates the presence of said antibodies.
Inventors
- 👤 Lance A. Liotta
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 17:52
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54366; G01N33/54386; G01N33/54387; G01N33/52; G01N33/525; Y; Y10; Y10S; Y10S435/00; Y10S435/805; Y10S435/97; Y10S436/00; Y10S436/807; Y10S436/81; Y10S436/815; Y10S436/817; Y10S436/818; Y10S436/82
Claims Analysis
Total Claims: 27
Claim 1
Independent Claim
108 words
A device for determining the presence of antigens which comprises: a matrix; a first zone in said matrix containing (a) bound and immobilized antigens and (b) enzyme-linked antibodies which are capable of immunologically reacting with said antigens, said antibodies being positioned in said first zone such that they will be removed from said first zone when reacted with antigens passing through said first zone but not removed from said first zone in the absence of such antigens; and a second zone removed from said first zone containing material capable of reacting with said enzyme-linked antibodies to produce a color forming reaction which indicates the presence of said antibodies.
Claim 2
Independent Claim
108 words
A device for determining the presence of antigens which comprises: a matrix; a first zone in said matrix containing (a) bound and immobilized antigens and (b) enzyme-linked antibodies which are capable of immunologically reacting with said antigens, said antibodies being positioned in said first zone such that they will be removed from said first zone when reacted with antigens passing through said first zone but not removed from said first zone in the absence of such antigens; and a second zone removed from said first zone containing material capable of reacting with said enzyme-linked antibodies to produce a color forming reaction which indicates the presence of said antibodies.
Claim 3
Dependent Claim
16 words
The device of claim 1 wherein said first and second zones are in a juxtapositioned relationship.
Claim 4
Dependent Claim
21 words
The device of claim 1 wherein said first zone contains enzyme-linked antibodies which are combined with bound and immobilized specific antigens.
Claim 5
Dependent Claim
36 words
The device of claim 1 wherein said first zone consists of at least two individual layers, with one of said layers containing enzyme-linked antibodies and another layer containing specific antigen bound and immobilized in said layer.
Claim 6
Dependent Claim
13 words
The device of claim 1 wherein said first zone is fabricated from nitrocellulose.
Claim 7
Dependent Claim
13 words
The device of claim 1 wherein said second zone is fabricated from nitrocellulose.
Claim 8
Dependent Claim
15 words
The device of claim 1 wherein said first zone is fabricated from a fibrous material.
Claim 9
Dependent Claim
15 words
The device of claim 1 wherein said second zone is fabricated from a fibrous material.
Claim 10
Dependent Claim
14 words
The device of claim 1 wherein said matrix is fabricated from a fibrous material.
Claim 11
Dependent Claim
12 words
The device of claim 9 wherein said fibrous material is diazobenzyloxymethyl paper.
Claim 12
Dependent Claim
16 words
The device of claim 1 wherein said first zone is fabricated from a porous polymer gel.
Claim 13
Dependent Claim
25 words
The device of claim 11 wherein said porous gel is at least one material selected from the group consisting of polyacrylamide, agarose and collagen gels.
Claim 14
Independent Claim
156 words
A method for determining the presence of antigens in a biological fluid which comprises: bringing a fluid which is to be tested for the presence of antigens into contact with a device having a matrix including a first zone containing (a) bound and immobilized antigens and (b) enzyme-linked antibodies which are capable of immunologically reacting with said antigens, said antibodies being positioned in said first zone such that they will be removed from said first zone but not removed from said first zone in the absence of such antigens, and a second zone removed from said first zone containing material capable of reacting with said enzyme-linked antibodies to produce a color forming reaction which indicates the presence of said antibodies; allowing said fluid to permeate said device; and observing the presence or absence of any color change in said second zone to thereby determine the presence or absence of the tested for antigen in said fluid.
Claim 15
Independent Claim
156 words
A method for determining the presence of antigens in a biological fluid which comprises: bringing a fluid which is to be tested for the presence of antigens into contact with a device having a matrix including a first zone containing (a) bound and immobilized antigens and (b) enzyme-linked antibodies which are capable of immunologically reacting with said antigens, said antibodies being positioned in said first zone such that they will be removed from said first zone but not removed from said first zone in the absence of such antigens, and a second zone removed from said first zone containing material capable of reacting with said enzyme-linked antibodies to produce a color forming reaction which indicates the presence of said antibodies; allowing said fluid to permeate said device; and observing the presence or absence of any color change in said second zone to thereby determine the presence or absence of the tested for antigen in said fluid.
Claim 16
Dependent Claim
16 words
The method of claim 13 wherein said first and second zones are in a juxtapositioned relationship.
Claim 17
Dependent Claim
36 words
The method of claim 13 wherein said first zone consists of at least two individual layers, with one of said layers containing enzyme-linked antibodies and another layer containing specific antigen bound and immobilized in said layer.
Claim 18
Dependent Claim
36 words
The method of claim 13 wherein said first zone consists of at least two individual layers, with one of said layers containing enzyme-linked antibodies and another layer containing specific antigen bound and immobilized in said layer.
Claim 19
Dependent Claim
13 words
The method of claim 13 wherein said first zone is fabricated from nitrocellulose.
Claim 20
Dependent Claim
13 words
The method of claim 13 wherein said second zone is fabricated from nitrocellulose.
Claim 21
Dependent Claim
23 words
The method of claim 13 wherein the antigen being determined is HCG and wherein the antigen in said device is human chorionic gonadatrophin.
Claim 22
Dependent Claim
23 words
The method of claim 13 wherein the antigen being determined is hepatitis antigen and wherein the antigen in said device is hepatitis antigen.
Claim 23
Dependent Claim
26 words
The method of claim 13 wherein the antigen being determined is rubella virus protein and wherein the antigen in said device is a rubella virus protein.
Claim 24
Independent Claim
99 words
A device for determining the presence of antibodies which comprises: a matrix; a first zone is said matrix containing enzyme-linked antigens and bound and immobilized antibodies which are capable of immunologically reacting with said enzyme-linked antigens, said enzyme-linked antigens being positioned in said first zone such that they will be removed from said first zone but not removed from said first zone in the absence of such antibodies; and a second zone removed from said first zone containing material capable of reacting with said enzyme-linked antigens to produce a color forming reaction which indicates the presence of said antigens.
Claim 25
Independent Claim
99 words
A device for determining the presence of antibodies which comprises: a matrix; a first zone is said matrix containing enzyme-linked antigens and bound and immobilized antibodies which are capable of immunologically reacting with said enzyme-linked antigens, said enzyme-linked antigens being positioned in said first zone such that they will be removed from said first zone but not removed from said first zone in the absence of such antibodies; and a second zone removed from said first zone containing material capable of reacting with said enzyme-linked antigens to produce a color forming reaction which indicates the presence of said antigens.
Claim 26
Independent Claim
165 words
A method for determining the presence of antibodies in a biological fluid which comprises: bringing a fluid which is to be tested for the presence of antibodies into contact with a device having a matrix including a first zone containing enzyme-linked antigens and bound and immobilized antibodies which are capable of immunologically reacting with said enzyme-linked antigens, said enzyme-linked antigens being positioned in said first zone such that they will be removed from said first zone when reacted with antibodies passing through said first zone but not removed from said first zone in the absence of such antibodies, and a second zone removed from said first zone containing material capable of reacting with said enzyme-linked antigens to produce a color forming reaction which indicates the presence of said antigens; allowing said fluid to permeate said device; and, observing the presence of absence of any color change in said second zone to thereby determine the presence or absence of the tested for antibodies in said fluid.
Claim 27
Independent Claim
165 words
A method for determining the presence of antibodies in a biological fluid which comprises: bringing a fluid which is to be tested for the presence of antibodies into contact with a device having a matrix including a first zone containing enzyme-linked antigens and bound and immobilized antibodies which are capable of immunologically reacting with said enzyme-linked antigens, said enzyme-linked antigens being positioned in said first zone such that they will be removed from said first zone when reacted with antibodies passing through said first zone but not removed from said first zone in the absence of such antibodies, and a second zone removed from said first zone containing material capable of reacting with said enzyme-linked antigens to produce a color forming reaction which indicates the presence of said antigens; allowing said fluid to permeate said device; and, observing the presence of absence of any color change in said second zone to thereby determine the presence or absence of the tested for antibodies in said fluid.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-05-01 | 1987-10-31 | 1988-05-01 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-05-01 | 1991-10-31 | 1992-05-01 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-05-01 | 1995-10-31 | 1996-05-01 | $7700.00 | Window Closed / Lapsed |