US4452734A — Herpes subunit vaccine

Claim 1

Independent Claim 82 words

An antigenic, immunogenic HSV2 subunit consisting essentially of 3 groups of viral directed, membrane-bound glycoproteins which are soluble in the presence of a nonionic or anionic surfactant, the first group being from about 110,000 to about 130,000 daltons, the second group being from about 83,000 to about 90,000 daltons and the third group being from about 55,000 to about 60,000 daltons, the subunit being free of detectable DNA and being capable of inducing neutralizing antibody and of being administered as a vaccine.

Claim 2

Dependent Claim 82 words

An antigenic, immunogenic HSV2 subunit consisting essentially of 3 groups of viral directed, membrane-bound glycoproteins which are soluble in the presence of a nonionic or anionic surfactant, the first group being from about 110,000 to about 130,000 daltons, the second group being from about 83,000 to about 90,000 daltons and the third group being from about 55,000 to about 60,000 daltons, the subunit being free of detectable DNA and being capable of inducing neutralizing antibody and of being administered as a vaccine.

Claim 3

Dependent Claim 10 words

A subunit according to claim 1 having about 11 polypeptides.

Claim 4

Dependent Claim 63 words

An antigenic, immunogenic HSV1 subunit consisting essentially of two groups of viral directed membrane-bound glycoproteins which are soluble in the presence of a nonionic or anionic surfactant, the first group being about 123,000 daltons and the second group being about 60,000 daltons, the subunit being free of detectable DNA and being capable of inducing neutralizing antibody and of being administered as a vaccine.

Claim 5

Dependent Claim 63 words

An antigenic, immunogenic HSV1 subunit consisting essentially of two groups of viral directed membrane-bound glycoproteins which are soluble in the presence of a nonionic or anionic surfactant, the first group being about 123,000 daltons and the second group being about 60,000 daltons, the subunit being free of detectable DNA and being capable of inducing neutralizing antibody and of being administered as a vaccine.

Claim 6

Dependent Claim 10 words

A subunit according to claim 3 having about 9 polypeptides.

Claim 7

Dependent Claim 74 words

A process of preparing an antigenic, immunogenic, herpes virus subunit comprising infecting the cells of a tissue culture with a herpes virus capable of growing in the tissue culture, incubating the infected tissue culture until a majority of the cells exhibit cytopathic effect, contacting the infected cells with a nonionic or anionic surfactant to obtain an extract, hydrolyzing substantially all of intact double-stranded DNA in the extract, and fractionating the extract by chromatographic means.

Claim 8

Dependent Claim 74 words

A process of preparing an antigenic, immunogenic, herpes virus subunit comprising infecting the cells of a tissue culture with a herpes virus capable of growing in the tissue culture, incubating the infected tissue culture until a majority of the cells exhibit cytopathic effect, contacting the infected cells with a nonionic or anionic surfactant to obtain an extract, hydrolyzing substantially all of intact double-stranded DNA in the extract, and fractionating the extract by chromatographic means.

Claim 9

Dependent Claim 20 words

A process according to claim 5 wherein the incubation is continued until about 75% of the cells exhibit cytopathic effect.

Claim 10

Dependent Claim 17 words

A process according to claim 5 wherein the surfactant is an aryl ether adduct of ethylene oxide.

Claim 11

Dependent Claim 16 words

A process according to claim 5 wherein the extraction is effected in the presence of salt.

Claim 12

Dependent Claim 18 words

A process according to claim 8 wherein the salt concentration is from about 0.1 to about 2 molar.

Claim 13

Dependent Claim 19 words

A process according to claim 5 wherein the hydrolysis of double stranded DNA is effected by treatment with DNase.

Claim 14

Dependent Claim 13 words

A process according to claim 5 wherein the chromatographic means comprise affinity chromatography.

Claim 15

Dependent Claim 15 words

A process according to claim 11 wherein the affinity chromatography is effected by a lectin.

Claim 16

Dependent Claim 17 words

A process according to claim 12 wherein the lectin is removed by treatment with a polymerized dextran.

Claim 17

Dependent Claim 12 words

A process according to claim 5 the chromatographic means comprise anion-exchange chromatography.

Claim 18

Dependent Claim 14 words

A process according to claim 14 wherein the anion-exchange chromatography is effected by DEAE-cellulose.

Claim 19

Dependent Claim 24 words

A process according to claim 5 wherein the fractionated extract is adsorbed on alum and the adsorbed extract is washed to remove unbound surfactant.

Claim 20

Dependent Claim 20 words

A process according to claim 5 wherein the virus is HSV1, HSV2, EBV, cytomegalovirus, varicella/zoster virus or simian B virus.

Claim 21

Dependent Claim 46 words

An antigenic, immunogenic HSV2 subunit having 3 groups of viral directed glycoproteins, a first group of from about 110,000 to about 130,000 daltons, a second group of from about 83,000 to about 90,000 daltons and a third group of from about 55,000 to about 60,000 daltons.

Claim 22

Dependent Claim 46 words

An antigenic, immunogenic HSV2 subunit having 3 groups of viral directed glycoproteins, a first group of from about 110,000 to about 130,000 daltons, a second group of from about 83,000 to about 90,000 daltons and a third group of from about 55,000 to about 60,000 daltons.

Claim 23

Dependent Claim 15 words

A subunit according to claim 18 having about 6 major and about 5 minor polypeptides.

Claim 24

Dependent Claim 31 words

An antigenic, immunogenic HSV1 subunit having a first group of viral directed glycoproteins of about 123,000 molecular weight and a second group of viral directed glycoproteins of about 60,000 molecular weight.

Claim 25

Dependent Claim 31 words

An antigenic, immunogenic HSV1 subunit having a first group of viral directed glycoproteins of about 123,000 molecular weight and a second group of viral directed glycoproteins of about 60,000 molecular weight.

Claim 26

Dependent Claim 15 words

A subunit according to claim 20 having about 3 major and about 6 minor polypeptides.

Annuity Stage	Window Opens	Payment Deadline	Surcharge Period Until	Estimated Fee	Status
3.5 Years (Year 4)	1987-06-05	1987-12-05	1988-06-05	$2000.00	Window Closed / Lapsed
7.5 Years (Year 8)	1991-06-05	1991-12-05	1992-06-05	$3760.00	Window Closed / Lapsed
11.5 Years (Year 12)	1995-06-05	1995-12-05	1996-06-05	$7700.00	Window Closed / Lapsed

Herpes subunit vaccine

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