Patent US4452893A successfully refreshed from Google Patents.
US4452893A
Expired / Lapsed
US
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Cell growth medium supplement
Assignee: Bayer Corp
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Filing: 1981-08-03
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Granted: 1984-06-05
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Expires: 2001-08-03
Abstract
Abstract Fraction IV, a discard fraction of the Cohn fractionation scheme, may be used as a supplement in cell growth media if the Fraction IV is rendered substantially free of components within the molecular weight range 2.5×10 5 -1.0×10 10 , which components inhibit cell growth.
Inventors
- 👤 Paul K. Ng
- 👤 Milton B. Dobkin
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 17:00
CPC Classifications
C; C12; C12N; C12N5/00; C12N5/0018; C12N5/0037; C12N2500/00; C12N2500/70; C12N2500/80; C12N2500/84; Y; Y10; Y10S; Y10S530/00; Y10S530/827; Y10S530/829; Y10S530/83; Y10S530/831
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
47 words
In a medium for the growth of mammalian cells containing nutrients and protein supplement, the improvement wherein the protein supplement consists entirely of human proteins and comprises Cohn Fraction IV treated to be substantially free of components within the molecular weight range 2.5×10 5 -1.0×10 10 .
Claim 2
Dependent Claim
47 words
In a medium for the growth of mammalian cells containing nutrients and protein supplement, the improvement wherein the protein supplement consists entirely of human proteins and comprises Cohn Fraction IV treated to be substantially free of components within the molecular weight range 2.5×10 5 -1.0×10 10 .
Claim 3
Dependent Claim
21 words
The composition of claim 1 further comprising vitamins, minerals, amino acids, carbohydrates, and other nutrients necessary for the growth of cells.
Claim 4
Dependent Claim
103 words
In a method for growing cells, comprising forming a cell growth medium containing vitamins, minerals, amino acids, carbohydrates, and other nutrients necessary for growth of cells and a cell growth supplement in an amount sufficient to grow the cells, seeding the cell growth medium with viable cells to be grown, inducing the growth of the cells in said medium, and recovering the cells from the medium, the improvement which comprises using Cohn Fraction IV which has been treated to be substantially free of growth inhibiting components within the molecular weight range of about 2.5×10 5 to 1.0×10 10 as the cell growth supplement.
Claim 5
Dependent Claim
103 words
In a method for growing cells, comprising forming a cell growth medium containing vitamins, minerals, amino acids, carbohydrates, and other nutrients necessary for growth of cells and a cell growth supplement in an amount sufficient to grow the cells, seeding the cell growth medium with viable cells to be grown, inducing the growth of the cells in said medium, and recovering the cells from the medium, the improvement which comprises using Cohn Fraction IV which has been treated to be substantially free of growth inhibiting components within the molecular weight range of about 2.5×10 5 to 1.0×10 10 as the cell growth supplement.
Claim 6
Independent Claim
68 words
A method for rendering Fraction IV obtained from human blood according to the Cohn fractionation method suitable as a supplement in cell culture media, which comprises (a) holding a aqueous mixture containing Fraction IV for a period of 1-2 hours and at a temperature of 2°-10° C. to render substantially all components which inhibit cell growth separable from the mixture; (b) separating the so-rendered components from the mixture.
Claim 7
Independent Claim
68 words
A method for rendering Fraction IV obtained from human blood according to the Cohn fractionation method suitable as a supplement in cell culture media, which comprises (a) holding a aqueous mixture containing Fraction IV for a period of 1-2 hours and at a temperature of 2°-10° C. to render substantially all components which inhibit cell growth separable from the mixture; (b) separating the so-rendered components from the mixture.
Claim 8
Dependent Claim
15 words
The method of claim 4 wherein the aqueous mixture is agitated during the holding period.
Claim 9
Dependent Claim
19 words
The method of claim 4 wherein the so-rendered components are separated from the mixture in Step b by centrifugation.
Claim 10
Dependent Claim
32 words
The method of claim 4 wherein the mixture in Step b is centrifuged for a period of time and at a velocity sufficient to separate all the so-rendered components from the mixture.
References Cited (Backward Citations)
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-06-05 | 1987-12-05 | 1988-06-05 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-06-05 | 1991-12-05 | 1992-06-05 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-06-05 | 1995-12-05 | 1996-06-05 | $7700.00 | Window Closed / Lapsed |