Patent US4455380A successfully refreshed from Google Patents.
US4455380A
Expired / Lapsed
US
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Process for determining tumor-associated glycolinkage
Assignee: Otsuka Pharmaceutical Co Ltd
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Filing: 1982-01-29
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Granted: 1984-06-19
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Expires: 2002-01-29
Abstract
Abstract A process for determining the level of TAG in a sample of body fluid which comprises reacting the TAG in a sample of body fluid with an AG-binding lectin or L-fucose-binding lectin to form a TAG-lectin complex and measuring the amount of the TAG-lectin complex or an unreacted lectin is disclosed. The process shows low cross-reactivity with hepatic diseases and is useful for diagnosis of cancers.
Inventors
- 👤 Masakazu Adachi
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 09:27
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/575; G01N33/5756; G01N33/58; Y; Y10; Y10S; Y10S436/00; Y10S436/807; Y10S436/808; Y10S436/811; Y10S436/813; Y10T; Y10T436/00; Y10T436/14; Y10T436/142222; Y10T436/143333
Claims Analysis
Total Claims: 34
Claim 1
Independent Claim
51 words
A process for determining the level of tumor-associated glycolinkage (TAG) in a sample of body fluid comprising reacting the TAG in a sample of body fluid with an N-acetyl-D-galactosamine (AG)-binding lectin or L-fucose-binding lectin to form a TAG-lectin complex and measuring the amount of the TAG-lectin complex or of unreacted lectin.
Claim 2
Dependent Claim
51 words
A process for determining the level of tumor-associated glycolinkage (TAG) in a sample of body fluid comprising reacting the TAG in a sample of body fluid with an N-acetyl-D-galactosamine (AG)-binding lectin or L-fucose-binding lectin to form a TAG-lectin complex and measuring the amount of the TAG-lectin complex or of unreacted lectin.
Claim 3
Dependent Claim
36 words
The process as claimed in claim 1, wherein said lectin is an AG-binding lectin, and said body fluid is blood, tissue fluid, lymph, hydrothorax, ascites, amniotic fluid, gastric juice, urine, pancreatic juice, cerebrospinal fluid or saliva.
Claim 4
Dependent Claim
36 words
The process as claimed in claim 1, wherein said lectin is L-fucose binding lectin, and said body fluid is blood, tissue fluid, lymph, hydrothorax, ascites, amniotic fluid, gastric juice, urine, pancreatic juice, cerebrospinal fluid or saliva.
Claim 5
Dependent Claim
84 words
The process as claimed in claim 2, wherein the reaction between the TAG and the lectin is carried out by competitively reacting the body fluid TAG to be measured and a definite quantity of an insolubilized TAG or insolubilized sugar derivative having an AG terminus with a definite quantity of labelled lectin, separating the insolubilized TAG or insolubilized sugar derivative having an AG terminus bound to labelled lectin and unbound lectin from each other, and measuring the labelling agent activity of either of them.
Claim 6
Dependent Claim
94 words
The process as claimed in claim 2, wherein the reaction between the TAG and the lectin is carried out by competitively reacting body fluid TAG to be measured and a definite quantity of labelled TAG or labelled sugar derivative having an AG terminus with a definite quantity of lectin or insolubilized lectin, separating the labelled TAG or sugar derivative having an AG terminus bound to lectin or insolubilized lectin and unbound labelled TAG or labelled sugar derivative having an AG terminus from each other, and measuring the labelling agent activity of either of them.
Claim 7
Dependent Claim
73 words
The process as claimed in claim 2, wherein the reaction between the TAG and the lectin is carried out by reacting the body fluid TAG to be measured with an insolubilized lectin to form a TAG-insolubilized lectin complex, reacting this complex with a definite quantity of labelled lectin, separating the complex bound to the labelled lectin and unbound labelled lectin from each other, and measuring the labelling agent activity of either of them.
Claim 8
Dependent Claim
27 words
The method of claim 4, 5, 6 or 2 which further comprises separating TAG from said body fluid sample and reacting the separated TAG with said lectin.
Claim 9
Dependent Claim
30 words
A process as claimed in claim 4 or 6 wherein said labelled lectin is lectin labelled with an enzyme selected from the group consisting of alkaline phosphatase β-galactosidase and hemeoctapeptide.
Claim 10
Dependent Claim
34 words
The process as claimed in claim 4 or 6 wherein said labelled lectin is a lectin labelled with a fluorescent substance selected from the group consisting of fluorescein, fluorescein isothiocyanate, rhodamine and dansyl chloride.
Claim 11
Dependent Claim
30 words
The process as claimed in claim 4 or 6 wherein said labelled lectin is lectin labelled with a radioactive substance selected from the group consisting of radioactive iodine and tritium.
Claim 12
Dependent Claim
36 words
The process as claimed in claim 2, wherein said lectin is a lectin selected from the group consisting of Dolichos bean lectin, braid orange lectin, Helix pomatia lectin, lima-bean lectin, Soybean lectin and Bauhinia bean lectin.
Claim 13
Dependent Claim
44 words
The process as claimed in claim 4, wherein said TAG in blood serum or blood plasma and an insolubilized porcine gastric mucous membrane (PGM), insolubilized by radiation-induced polymerization with a hydroxyethyl methacrylate (HEMA) or polystyrene bead, are competitively reacted with a peroxidase labelled DBA.
Claim 14
Dependent Claim
65 words
The process as claimed in claim 4 or 5, wherein said sugar derivative having an AG terminus is selected from the group consisting of sulfate glycopeptide type A of porcine gastric mucous membrane, cytolipins K and R of human erythrocyte membrane, asialo derivative of human blood active substance type A, mucin type A + of porcine submandibular membrane, asialo GM 1 and Forssman antigenic substance.
Claim 15
Dependent Claim
24 words
The process as claimed in claim 5, wherein said TAG in blood serum or blood plasma and 125 I-PGM are competitively reacted with DBA.
Claim 16
Dependent Claim
31 words
The process as claimed in claim 5, wherein said body fluid is blood serum or blood plasma and said AG-binding lectin is a Dolichos bean lectin (DBA) labelled with a peroxidase.
Claim 17
Dependent Claim
32 words
The process as claimed in claim 6, wherein said TAG in blood serum or blood plasma is reacted with DBA-agarose and the resulting complex is further reacted with a peroxidase labelled DBA.
Claim 18
Dependent Claim
19 words
The process as claimed in claim 3, wherein said lectin is Lotus tetragonolobus lectin (LTL) or Ulex europeus lectin.
Claim 19
Dependent Claim
84 words
The process as claimed in claim 3, wherein the reaction between the TAG and the lectin is carried out by competititely reacting the body fluid TAG to be measured and a definite quantity of an insolubilized TAG or insolubilized sugar derivative having an L-fucose terminus with a definite quantity of labelled lectin, separating the insolubilized TAG or insolubilized sugar derivative having an L-fucose terminus bound to labelled lectin and unbound lectin from each other, and measuring the labelling agent activity of either of them.
Claim 20
Dependent Claim
95 words
The process as claimed in claim 3, wherein the reaction between the TAG as the lectin is carried out by competitively reacting body fluid TAG to be measured and a definite quantity of labelled TAG or labelled sugar derivative having an L-fucose terminus with a definite quantity of lectin or insolubilized lectin, separating the labelled TAG or labelled sugar derivative having an L-fucose terminus bound to lectin or insolubilized lectin and unbound labelled TAG or labelled sugar derivative having an L-fucose terminus from each other, and measuring the labelling agent activity of either of them.
Claim 21
Dependent Claim
73 words
The process as claimed in claim 3, wherein the reaction between the TAG and the lectin is carried out by reacting the body fluid TAG to be measured with an insolubilized lectin to form a TAG-insolubilized lectin complex, reacting this complex with a definite quantity of labelled lectin, separating the complex bound to the labelled lectin and unbound labelled lectin from each other, and measuring the labelling agent activity of either of them.
Claim 22
Dependent Claim
26 words
The process as claimed in claim 3, wherein said body fluid is blood serum or blood plasma, and said L-fucose binding lectin is peroxidase labelled LTL.
Claim 23
Dependent Claim
64 words
The process as claimed in claim 18 or 19, wherein said sugar derivative having an L-fucose terminus is a sugar derivative selected from the group consisting of human blood active substance type Le a and Le b , sulfated glycoprotein type A of porcine gastric mucous membrane, sulfated glycoprotein active substance type H(O) of porcine gastric mucous membrane and human erythrocyte H 1 antigen.
Claim 24
Dependent Claim
35 words
The process as claimed in claim 18 or 20, wherein said labelled lectin is a lectin labelled with an enzyme selected from the group consisting of glucoamylase, glucose oxidase, peroxidase, alkaline phosphatase, β-galactosidase and hemeoctapeptide.
Claim 25
Dependent Claim
33 words
The process as claimed in claim 18 or 20, wherein said labelled lectin is a lectin labelled with a fluorescent substance selected from the group consisting of fluorescein isocyanate, rhodamine and dansyl chloride.
Claim 26
Dependent Claim
31 words
The process as claimed in claim 18 or 20, wherein said labelled lectin is a lectin labelled with a radioactive substance selected from the group consisting of radioactive iodine and tritium.
Claim 27
Dependent Claim
43 words
The process as claimed in claim 18, wherein said TAG in blood serum or blood plasma and an insolubilized porcine gastric mucous membrane (PGM), insolubilized by radiation-induced polymerization with a hydroxyethyl methacrylate (HEMA) or polystyrene bead, are competitively reacted with peroxidase labelled LTL.
Claim 28
Dependent Claim
24 words
The process as claimed in claim 19, wherein said TAG in blood serum or blood plasma and 125 I-PGM are competitively reacted with LTL.
Claim 29
Dependent Claim
31 words
The process as claimed in claim 20, wherein said TAG in blood serum or blood plasma is reacted with LTL-agarose and the resulting complex is further reacted with peroxidase labelled LTL.
Claim 30
Independent Claim
51 words
A method for diagnosing cancer, comprising measuring the level of tumor-associated glycolinkage in body fluid of a subject according to the method described in any one of claims 1, 15, 12, 14, 16, 21, 26, 27 or 28 and comparing the thus-measured level with that of a person in normal health.
Claim 31
Dependent Claim
23 words
A kit for determining TAG level in a body fluid comprising AG-binding lectin or L-fucose-binding lectin as a specific agglutinating agent for TAG.
Claim 32
Dependent Claim
23 words
A kit for determining TAG level in a body fluid comprising AG-binding lectin or L-fucose-binding lectin as a specific agglutinating agent for TAG.
Claim 33
Dependent Claim
15 words
The kit as claimed in claim 30, wherein said specific agglutinating agent is AG-binding lectin.
Claim 34
Dependent Claim
16 words
The kit as claimed in claim 30, wherein said specific agglutinating agent is L-fucose binding lectin.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-06-19 | 1987-12-19 | 1988-06-19 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-06-19 | 1991-12-19 | 1992-06-19 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-06-19 | 1995-12-19 | 1996-06-19 | $7700.00 | Window Closed / Lapsed |