Wound healing glycoside compositions

Claim 1

A composition for stimulating wound healing by promoting proliferation of epithelium cells in the final phase of wound healing comprising 1 to 10 μg of a glycosteroid isolated from a protein-free fraction of mammalian tissue or fluid in a pharmaceutically acceptable carrier, said glycosteroid having the structure: ##STR8## wherein R is an oligosaccharide containing five sugar units wherein the glycosteroid is isolated from mammalian tissue or fluid by: (a) homogenizing the mammalian tissue or fluid with an aqueous solution of 0.2% NaCl; (b) clearing the homogenate from the cellular debris by centrifugation or filtration; (c) thereafter ultra-filtering the cleared homogenate; (d) concentrating the ultrafiltrate at 40° C. until the ionic strength thereof corresponds to a 0.8% NaCl solution; (e) binding orgnic material in the concentrated ultrafiltrate to active carbon; (f) thereafter extracting the active carbon with 10-30% acetic acid; (g) evaporating the solvent from the extract; (h) dissolving the residue in 0.2-2M acetic acid; (i) chromatographing the resulting solution over a molecular sieve column; (j) thereafter eluting the column with 0.2-2M acetic acid and collecting the second fraction with cell proliferating activity; and (k) removing the solvent from the collected eluate.