US4296233A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Conjugate of amino sugar-steroid hormone
Assignee: Kureha Corp
•
Filing: 1979-03-29
•
Granted: 1981-10-20
•
Expires: 1999-03-29
Abstract
Abstract Conjugates of amino sugar-steroid hormone are prepared by binding an amino sugar to steroid hormone at 3- or 17-position by a direct reaction or by binding with a binding agent, and the conjugate is useful in an enzyme immunoassay method.
Inventors
- 👤 Satoru Enomoto
- 👤 Kiro Asano
- 👤 Hiromitsu Tanaka
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 01:36
CPC Classifications
C; C07; C07J; C07J41/00; C07J41/0033; C07J41/0088; C07J1/00; C07J1/0003; C07J1/0011; C07J1/0051; C07J1/0066; C07J1/007; C07J1/0074; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/74; G01N33/743; Y; Y10; Y10S; Y10S435/00; Y10S435/962; Y10S436/00; Y10S436/815; Y10S436/817
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
115 words
A conjugate of an amino sugar and a steroid hormone having the formula: ##STR6## wherein R 1 C is a radical of a carboxyl group containing amino sugar selected from the group consisting of 2-amino-2-deoxy-glucuronic acid, 2-amino-2-deoxy-3-O-(1-carboxyethyl) D-glucose and 2-amino-2-deoxy-3-O-β-D-glucopyranurosyl-D-galactose; R 2 is a radical of a steroid hormone selected from the group consisting of 17β-hydroxy-4-androsten-3-one; 3β-hydroxy-5-androsten-17-one; 1,3,5 (10)-estratriene-3,17 β-diol; 5β-pregnane-3α, 20α-diol; 4-pregnene-3, 20-dione; 11β, 17α, 21-trihydroxy-4-pregnene-3, 20-dione; 11β, 21-dihydroxy-4-pregnene-3,20-dione; prednisone; desoxymethasone, mestanolone; androstanolone; unsodeoxycholic acid methanedienone; β-sitosterol and Alphaxalone, the bonding between said amino sugar radical and said steroid hormone radical occurring only at the 3 or 17 position of said steroid radical; m is 0 or 1 and n is 1 or 2.
Claim 2
Dependent Claim
115 words
A conjugate of an amino sugar and a steroid hormone having the formula: ##STR6## wherein R 1 C is a radical of a carboxyl group containing amino sugar selected from the group consisting of 2-amino-2-deoxy-glucuronic acid, 2-amino-2-deoxy-3-O-(1-carboxyethyl) D-glucose and 2-amino-2-deoxy-3-O-β-D-glucopyranurosyl-D-galactose; R 2 is a radical of a steroid hormone selected from the group consisting of 17β-hydroxy-4-androsten-3-one; 3β-hydroxy-5-androsten-17-one; 1,3,5 (10)-estratriene-3,17 β-diol; 5β-pregnane-3α, 20α-diol; 4-pregnene-3, 20-dione; 11β, 17α, 21-trihydroxy-4-pregnene-3, 20-dione; 11β, 21-dihydroxy-4-pregnene-3,20-dione; prednisone; desoxymethasone, mestanolone; androstanolone; unsodeoxycholic acid methanedienone; β-sitosterol and Alphaxalone, the bonding between said amino sugar radical and said steroid hormone radical occurring only at the 3 or 17 position of said steroid radical; m is 0 or 1 and n is 1 or 2.
Claim 3
Dependent Claim
44 words
A process for producing the amino sugar-steroid hormone conjugate of claim 1, comprising: reacting a carboxyl group containing amino sugar with a steroid hormone, said reaction occurring between the carboxyl group of said amino sugar and the 3- or 17-position of said steroid hormone.
Claim 4
Dependent Claim
20 words
The process of claim 2, wherein said carboxyl group containing amino sugar is in the form of its silver salt.
Claim 5
Dependent Claim
71 words
A process for producing the amino sugar-steroid hormone conjugate of claim 1, comprising; reacting a carboxyl group containing amino sugar with said steroid hormone at the 3- or 17-position of said steroid hormone in the presence of a binding agent, said binding agent being selected from the group consisting of: X(CH.sub.2).sub.n COOH, X(CH.sub.2).sub.n COX, HOOC(CH.sub.2).sub.n COOH, and XOC(CH.sub.2).sub.n COX wherein n is 1 or 2 and X is a halogen atom.
Claim 6
Dependent Claim
10 words
The process of claim 4, wherein said conjugate is acetylized.
References Cited (Backward Citations)
US3427300A
Cited
US3678029A
Cited
US3836520A
Cited
US4003998A
Cited
US3963697A
Cited
US4021535A
Cited
US4088757A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-10-19 | 1985-04-20 | 1985-10-20 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-10-19 | 1989-04-20 | 1989-10-20 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-10-19 | 1993-04-20 | 1993-10-20 | $7700.00 | Window Closed / Lapsed |