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US4464471A
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Biologically engineered plasmid coding for production of β-glucosidase, organisms modified by this plasmid and methods of use
Assignee: University of Cincinnati
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Filing: 1982-02-01
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Granted: 1984-08-07
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Expires: 2002-02-01
Abstract
Abstract A biologically engineered plasmid coding for the production of β-glucosidase. The plasmid can be incorporated into various microorganisms to enable the microorganism to digest cellobiose, which is produced from cellulose by an endo- or exocellular cellulase. One particular application is the incorporation of this plasmid into a microorganism which produces ethanol. Preferably, this ethanol producing microorganism is also ethanol tolerant.
Inventors
- 👤 Richard W. Armentrout
- 👤 Ronald D. Brown
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 08:09
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/24; C12N9/2402; C12N9/2405; C12N9/2434; C12N9/2437; C12N9/2445; C12P; C12P7/00; C12P7/02; C12P7/04; C12P7/06; C12P7/08; C12P7/10; C12Y; C12Y302/00; C12Y302/01; C12Y302/01004; C12Y302/01021; Y; Y02; Y02E; Y02E50/00; Y02E50/10
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
36 words
A recombination DNA plasmid comprising a cloning vector having covalently bound thereto a DNA insert coding for the production of beta-glucosidase wherein said DNA insert coding for the production of beta-glucosidase is isolated from Esherichia adecarboxylata.
Claim 2
Dependent Claim
36 words
A recombination DNA plasmid comprising a cloning vector having covalently bound thereto a DNA insert coding for the production of beta-glucosidase wherein said DNA insert coding for the production of beta-glucosidase is isolated from Esherichia adecarboxylata.
Claim 3
Dependent Claim
28 words
Esherichia coli comprising a recombinant DNA cloning vector having covalently bound thereto a DNA insert coding for the production of beta-glucosidase, said DNA insert isolated from Esherichia adecarboxylata.
Claim 4
Dependent Claim
28 words
Esherichia coli comprising a recombinant DNA cloning vector having covalently bound thereto a DNA insert coding for the production of beta-glucosidase, said DNA insert isolated from Esherichia adecarboxylata.
References Cited (Backward Citations)
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US4275163A
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US4220721A
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GB2055121A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-08-07 | 1988-02-06 | 1988-08-07 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-08-07 | 1992-02-06 | 1992-08-07 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-08-07 | 1996-02-06 | 1996-08-07 | $7700.00 | Window Closed / Lapsed |