Patent US947996A successfully refreshed from Google Patents.
Patent US4468456A successfully refreshed from Google Patents.
US4468456A
Expired / Lapsed
US
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Medium for differentiating Streptococcus mutans
Assignee: Warner Lambert Co LLC
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Filing: 1982-03-29
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Granted: 1984-08-28
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Expires: 2002-03-29
Abstract
Abstract A medium for the selective growth and identification of Streptococcus mutans bacteria is disclosed, that includes a tryptone-glucose extract agar, monobasic and dibasic potassium phosphate, yeast extract, agar, a color indicator for the bacteria and a solution of sucrose ranging in concentration from 1% to 15%. Preferably, the concentration of the sucrose may range from about 3% to about 11%. A bacteria culture plate may be prepared comprising the medium, and may include a first basal layer comprised entirely of the medium, and a second overlayer agar coating, including a mixture of the medium with a calcium phosphate suspension. Both the basal layer and the overlayer agar coating are preferably adjusted to a mildly basic pH. The present medium, and bacteria culture plates prepared therewith, offer desired bacterial specificity with no growth inhibition, that is usually the case with specific media of this kind. The present medium and bacteria culture plate is particularly useful, as Streptococcus mutans is considered to be instrumental in dental caries development.
Inventors
- 👤 John L. Deyloff
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 14:26
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/02; C12Q1/04; C12Q1/14; C12N; C12N1/00; C12N1/20; C12N1/205; C12R; C12R2001/00; C12R2001/01; C12R2001/46; G; G01; G01N; G01N2333/00; G01N2333/195; G01N2333/315; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/885
Claims Analysis
Total Claims: 49
Claim 1
Independent Claim
170 words
A method for the selective identification of Streptococcus mutans comprising: A. preparing a medium consisting essentially of: 1. tryptone-glucose extract agar; 2. dibasic potassium phosphate; 3. monobasic potassium phosphate; 4. yeast extract; 5. agar; 6. a color indicator for said bacteria; and 7. from about 1% to about 15% sucrose; B. placing a quantity of said medium in a receptacle to form a first basal layer; C. permitting said first basal layer to solidify; D. preparing an overlayer agar coating comprising a mixture of a suspension of calcium phosphate, and a sterile quantity of said medium; E. disposing a quantity of said overlayer agar coating on said first basal layer to form a product, wherein the amount of calcium phosphate is sufficient to detect production of acid by Streptococcus Mutans. F. drying the product to form a culture plate ready for use; G. inoculating and incubating under conditions suitable for growth the culture plate with a sample to be determined; and H. Observing the incubated plate to identify Streptococcus Mutans.
Claim 2
Independent Claim
170 words
A method for the selective identification of Streptococcus mutans comprising: A. preparing a medium consisting essentially of: 1. tryptone-glucose extract agar; 2. dibasic potassium phosphate; 3. monobasic potassium phosphate; 4. yeast extract; 5. agar; 6. a color indicator for said bacteria; and 7. from about 1% to about 15% sucrose; B. placing a quantity of said medium in a receptacle to form a first basal layer; C. permitting said first basal layer to solidify; D. preparing an overlayer agar coating comprising a mixture of a suspension of calcium phosphate, and a sterile quantity of said medium; E. disposing a quantity of said overlayer agar coating on said first basal layer to form a product, wherein the amount of calcium phosphate is sufficient to detect production of acid by Streptococcus Mutans. F. drying the product to form a culture plate ready for use; G. inoculating and incubating under conditions suitable for growth the culture plate with a sample to be determined; and H. Observing the incubated plate to identify Streptococcus Mutans.
Claim 3
Dependent Claim
19 words
The method of claim 1 wherein said sucrose ranges in concentration from about 3% to about 11% by weight.
Claim 4
Dependent Claim
17 words
The method of claim 2 wherein said sucrose is present in a concentration of 5 wt. %.
Claim 5
Independent Claim
72 words
The method of claims 1, 2 or 3 wherein said tryptone-glucose extract agar is present in an amount of 2.35 wt. %, said monobasic potassium phosphate is present in an amount of 0.12 wt. %, said dibasic potassium phosphate is present in an amount of 0.12 wt. %, said yeast extract is present in an amount of 0.25 wt. %, and said agar is present in an amount of 0.5 wt. %.
Claim 6
Dependent Claim
11 words
The method of claim 4 wherein said indicator comprises bromcresol purple.
Claim 7
Dependent Claim
17 words
The method of claim 5 wherein said indicator is present in an amount of 0.0032 wt. %.
Claim 8
Dependent Claim
19 words
The method of claim 4 wherein said suspension comprises a filtered mixture of calcium chloride and potassium hydrogen phosphate.
Claim 9
Dependent Claim
30 words
The method of claim 1 wherein said suspension is prepared by sequentially passing said calcium chloride and said potassium hydrogen phosphate through a filter medium, and into a sterile container.
Claim 10
Dependent Claim
29 words
The method of claim 4 wherein the mixture of said overlayer agar coating comprises 100 parts by volume of said medium and 15 parts by volume of said suspension.
Claim 11
Dependent Claim
33 words
The method of claim 4 wherein said basal layer and said overlayer agar coating are disposed in said receptacle, in a volumetric ratio with respect to each other ranging up to about 3:1.
Claim 12
Dependent Claim
22 words
The method of claim 4 wherein said basal layer and said overlayer agar coating are individually adjusted to a mildly basic pH.
Claim 13
Dependent Claim
18 words
The method of claim 11 wherein said mildly basic pH comprises a pH ranging between 7.0 and 8.0.
Claim 14
Independent Claim
14 words
The method of claims 1, 2 or 3 wherein said indicator comprises bromcresol purple.
Claim 15
Dependent Claim
17 words
The method of claim 13 wherein said indicator is present in an amount of 0.0032 wt. %.
Claim 16
Independent Claim
22 words
The method of claims 1, 2 or 3 wherein said suspension comprises a filtered mixture of calcium chloride and potassium hydrogen phosphate.
Claim 17
Independent Claim
32 words
The method of claims 1, 2 or 3 wherein the mixture of said overlayer agar coating comprises 100 parts by volume of said medium and 15 parts by volume of said suspension.
Claim 18
Independent Claim
36 words
The method of claims 1, 2 or 3 wherein said basal layer and said overlayer agar coating are disposed in said receptacle, in a volumetric ratio with respect to each other ranging up to about 3:1.
Claim 19
Independent Claim
25 words
The method of claims 1, 2 or 3 wherein said basal layer and said overlayer agar coating are individually adjusted to a mildly basic pH.
Claim 20
Dependent Claim
18 words
The method of claim 18 wherein said mildly basic pH comprises a pH ranging between 7.0 and 8.0.
Claim 21
Dependent Claim
112 words
A bacterial culture plate for the selective differentiation of Streptococcus Mutans bacteria comprising: A. a receptacle; B. a first basal layer comprising a medium, said medium consisting essentially of: 1. tryptone-glucose extract agar; 2. dibasic potassium phosphate; 3. monobasic potassium phosphate; 4. yeast extract; 5. agar; 6. a color indicator for said bacteria; and 7. from about 1% to about 15% by weight of sucrose; C. an overlayer agar coating disposed over said first basal layer, said overlayer agar coating comprising a mixture of a suspension of calcium phosphate, and a sterile quantity of said medium wherein the amount of calcium phosphate is sufficient to detect production of acid by Streptococcus Mutans.
Claim 22
Dependent Claim
112 words
A bacterial culture plate for the selective differentiation of Streptococcus Mutans bacteria comprising: A. a receptacle; B. a first basal layer comprising a medium, said medium consisting essentially of: 1. tryptone-glucose extract agar; 2. dibasic potassium phosphate; 3. monobasic potassium phosphate; 4. yeast extract; 5. agar; 6. a color indicator for said bacteria; and 7. from about 1% to about 15% by weight of sucrose; C. an overlayer agar coating disposed over said first basal layer, said overlayer agar coating comprising a mixture of a suspension of calcium phosphate, and a sterile quantity of said medium wherein the amount of calcium phosphate is sufficient to detect production of acid by Streptococcus Mutans.
Claim 23
Dependent Claim
21 words
The bacterial culture plate of claim 20 wherein said sucrose ranges in concentration from about 3% to about 11% by weight.
Claim 24
Dependent Claim
19 words
The bacterial culture plate of claim 21 wherein said sucrose is present in a concentration of 5% by weight.
Claim 25
Dependent Claim
74 words
The bacterial culture plate of claims 20, 21 or 22 wherein said tryptone-glucose extract agar is present in an amount of 2.35 wt. %, said monobasic potassium phosphate is present in an amount of 0.12 wt. %, said dibasic potassium phosphate is present in an amount of 0.12 wt. %, said yeast extract is present in an amount of 0.25 wt. %, and said agar is present in an amount of 0.5 wt. %.
Claim 26
Dependent Claim
13 words
The bacterial culture plate of claim 23 wherein said indicator comprises bromcresol purple.
Claim 27
Dependent Claim
16 words
The medium of claim 24 wherein said indicator is present in an amount of 0.0032 wt.%.
Claim 28
Dependent Claim
26 words
The bacterial culture plate of claim 25 wherein said mixture comprises 100 parts by volume of said medium and 15 parts by volume of said suspension.
Claim 29
Dependent Claim
35 words
The bacterial culture plate of claim 25 wherein said basal layer and said overlayer agar coating are present in said receptacle, in a volumetric ratio with respect to each other ranging up to about 3:1.
Claim 30
Dependent Claim
23 words
The bacterial culture plate of claim 25 wherein said basal layer and said overlayer agar coating are adjusted to a mildly basic pH.
Claim 31
Dependent Claim
26 words
The bacterial culture plate of claim 23 wherein said mixture comprises 100 parts by volume of said medium and 15 parts by volume of said suspension.
Claim 32
Dependent Claim
35 words
The bacterial culture plate of claim 23 wherein said basal layer and said overlayer agar coating are present in said receptacle, in a volumetric ratio with respect to each other ranging up to about 3:1.
Claim 33
Dependent Claim
23 words
The bacterial culture plate of claim 30 wherein said basal layer and said overlayer agar coating are adjusted to a mildly basic pH.
Claim 34
Dependent Claim
23 words
The bacterial culture plate of claim 23 wherein said basal layer and said overlayer agar coating are adjusted to a mildly basic pH.
Claim 35
Dependent Claim
16 words
The bacterial culture plate of claims 20, 21 or 22 wherein said indicator comprises bromcresol purple.
Claim 36
Dependent Claim
18 words
The bacterial culture plate of claim 33 wherein said indicator is present in an amount of 0.0032 wt.%.
Claim 37
Dependent Claim
26 words
The bacterial culture plate of claim 34 wherein said mixture comprises 100 parts by volume of said medium and 15 parts by volume of said suspension.
Claim 38
Dependent Claim
23 words
The bacterial culture plate of claim 34 wherein said basal layer and said overlayer agar coating are adjusted to a mildly basic pH.
Claim 39
Dependent Claim
26 words
The bacterial culture plate of claim 33 wherein said mixture comprises 100 parts by volume of said medium and 15 parts by volume of said suspension.
Claim 40
Dependent Claim
35 words
The bacterial culture plate of claim 33 wherein said basal layer and said overlayer agar coating are present in said receptacle, in a volumetric ratio with respect to each other ranging up to about 3:1.
Claim 41
Dependent Claim
23 words
The bacterial culture plate of claim 33 wherein said basal layer and said overlayer agar coating are adjusted to a mildly basic pH.
Claim 42
Dependent Claim
24 words
The bacterial culture plate of claims 20, 21 or 22 wherein said suspension comprises a filtered mixture of calcium chloride and potassium hydrogen phosphate.
Claim 43
Dependent Claim
29 words
The bacterial culture plate of claims 20, 21 or 22 wherein said mixture comprises 100 parts by volume of said medium and 15 parts by volume of said suspension.
Claim 44
Dependent Claim
35 words
The bacterial culture plate of claim 41 wherein said basal layer and said overlayer agar coating are present in said receptacle, in a volumetric ratio with respect to each other ranging up to about 3:1.
Claim 45
Dependent Claim
23 words
The bacterial culture plate of claim 41 wherein said basal layer and said overlayer agar coating are adjusted to a mildly basic pH.
Claim 46
Dependent Claim
38 words
The bacterial culture plate of claims 20, 21 or 22 wherein said basal layer and said overlayer agar coating are present in said receptacle, in a volumetric ratio with respect to each other ranging up to about 3:1.
Claim 47
Dependent Claim
23 words
The bacterial culture plate of claim 44 wherein said basal layer and said overlayer agar coating are adjusted to a mildly basic pH.
Claim 48
Dependent Claim
26 words
The bacterial culture plate of claims 20, 21 or 22 wherein said basal layer and said overlayer agar coating are adjusted to a mildly basic pH.
Claim 49
Dependent Claim
20 words
The bacterial culture plate of claim 46 wherein said mildly basic pH comprises a pH ranging between 7.0 and 8.0.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-08-28 | 1988-02-27 | 1988-08-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-08-28 | 1992-02-27 | 1992-08-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-08-28 | 1996-02-27 | 1996-08-28 | $7700.00 | Window Closed / Lapsed |