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US4495176A
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Phosphopeptides from casein-based material
Assignee: Institut National de la Recherche Agronomique
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Filing: 1982-06-16
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Granted: 1985-01-22
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Expires: 2002-06-16
Abstract
Abstract Phosphopeptides useful as alimentary products or as medicaments are obtained by a method of subjecting phosphocaseinates of monovalent cations or paracasein derived therefrom to enzymatic hydrolysis with at least one proteolytic enzyme that simulates proteic digestion in vivo in the human body, ultrafiltering the resultant hydrolysate with a membrane that retains the enzyme to obtain a permeate containing phosphopeptides and non-phosphorylated peptides, adding to the permeate a bivalent cation salt to form aggregates of the phosphopeptides, subjecting the resultant solution to ultrafiltration with a membrane that retains the phosphopeptide aggregates, and recovering the retained phosphopeptides. The phosphopeptides form salts, which have dietetic uses, with macroelements such as calcium and/or magnesium and/or oligoelements such as iron and zinc.
Inventors
- 👤 Gerard Brule
- 👤 Loic Roger
- 👤 Jacques Fauquant
- 👤 Michel Piot
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 14:18
CPC Classifications
A; A61; A61K; A61K38/00; A61K38/01; A61K38/012; A61K38/018; A23; A23J; A23J3/00; A23J3/30; A23J3/32; A23J3/34; A23J3/341; A23J3/343; A23J3/344; A23L; A23L33/00; A23L33/10; A23L33/17; A23L33/19
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
178 words
Products consisting of organophosphorated salts formed by phosphopeptides and linked bivalent cations produced by the process comprising subjecting a casein-based material containing phosphocaseinates of monovalent cations or paracasein derived therefrom to an enzymatic hydrolysis at a pH in the range of 7 to 9 and a temperature in the range of 37° to 45° C. by means of at least one proteolytic enzyme capable of substantially reproducing the proteic digestion which occurs in vivo in the human body, subjecting the resultant hydrolyzate to at least one ultrafiltration step on membranes which retain the enzyme but allow all the peptides of the hydrolyzate to pass in the permeate to obtain a permeate containing phosphopeptides and non-phosphorylated peptides, adding to the permeate at least one bivalent cation salt to form aggregates of the phosphopeptides to produce a solution containing essentially the aggregates of phosphopeptides and the non-phosphorylated peptides, subjecting the solution to at least one ultrafiltration step with membranes that retain the aggregates of phosphopeptides and allow the non-phosphorylated peptides to pass through, and recovering the retained aggregates of phosphopeptides.
Claim 2
Dependent Claim
178 words
Products consisting of organophosphorated salts formed by phosphopeptides and linked bivalent cations produced by the process comprising subjecting a casein-based material containing phosphocaseinates of monovalent cations or paracasein derived therefrom to an enzymatic hydrolysis at a pH in the range of 7 to 9 and a temperature in the range of 37° to 45° C. by means of at least one proteolytic enzyme capable of substantially reproducing the proteic digestion which occurs in vivo in the human body, subjecting the resultant hydrolyzate to at least one ultrafiltration step on membranes which retain the enzyme but allow all the peptides of the hydrolyzate to pass in the permeate to obtain a permeate containing phosphopeptides and non-phosphorylated peptides, adding to the permeate at least one bivalent cation salt to form aggregates of the phosphopeptides to produce a solution containing essentially the aggregates of phosphopeptides and the non-phosphorylated peptides, subjecting the solution to at least one ultrafiltration step with membranes that retain the aggregates of phosphopeptides and allow the non-phosphorylated peptides to pass through, and recovering the retained aggregates of phosphopeptides.
Claim 3
Dependent Claim
37 words
The product according to claim 1, wherein the bivalent cations contained in said organophosphorated salts are at least partially replaced by oligoelements selected from the group consisting of iron, zinc, copper, chromium, nickel, cobalt, manganese and selenium.
Claim 4
Dependent Claim
26 words
The product according to claim 1 wherein the phosphocaseinates are selected from the group consisting of sodium, potassium and ammonium phosphocaseinates or derivatives of said phosphocaseinates.
Claim 5
Dependent Claim
48 words
The product according to claim 1, wherein said casein-based material contains paracasein obtained by treating the monovalent cation phosphocaseinate with rennet, precipitating the paracasein contained in the resultant hydrolysis product by acidification to pH 4.6, and separating the precipitated paracasein from the supernatant solution which contains a caseinomacropeptide.
Claim 6
Dependent Claim
32 words
The product according to claim 1, wherein the enzymatic hydrolysis step is carried out in a device which combines an ultrafiltration means with an enzymatic reactor, to form a membrane enzymatic reactor.
Claim 7
Dependent Claim
64 words
The product according to claim 1, wherein enzymatic hydrolysis is carried out continuously by feeding the casein-based material to a reaction zone so as to cause its intimate contact with the enzyme, withdrawing the reaction product continuously from the reaction zone, continuously passing the reaction product to an ultrafiltration zone, and continuously withdrawing a permeate from the ultrafiltration zone which constitutes the peptidic hydrolyzate.
Claim 8
Dependent Claim
22 words
The product according to claim 1, wherein the pH is adjusted in the range of 7 to 8.5, during the enzymatic hydrolysis.
Claim 9
Dependent Claim
28 words
The product according to claim 1, wherein the proteolytic enzyme is pancreatin in the form of a natural pancreatic extract, or a synthetic mixture of trypsine and alphachymotrypsine.
Claim 10
Dependent Claim
20 words
The product according to claim 1, wherein the hydrolysis temperature is selected in the range of 37° to 40° C.
Claim 11
Dependent Claim
20 words
A method of effecting nutritional therapy which comprises administering an effective amount of the product of claim 1 or 2.
Claim 12
Dependent Claim
28 words
The aliment composition for dietetic nutrition comprising an effective amount of a product according to claim 1 or 2, and an excipient suitable for oral or enteral nutrition.
Claim 13
Dependent Claim
17 words
The aliment composition according to claim 11, which further comprises a complementary source of essential amino acids.
Claim 14
Dependent Claim
20 words
A method of effecting medicinal therapy which comprises administering an effective amount of the products of claim 1 or 2.
Claim 15
Dependent Claim
23 words
A pharmaceutical composition comprising an effective amount of a product according to claim 1 or 2, and an excipient suitable for enteral administration.
Claim 16
Dependent Claim
35 words
A pharmaceutical composition comprising an effective amount of at least one organophosphorated salt of a phosphopeptide according to claim 1, which comprises a mineraloprotidic supplement comprising organic phosphorus, calcium and magnesium or a mixture thereof.
Claim 17
Dependent Claim
16 words
A pharmaceutical composition comprising an effective amount of at least one phosphopeptide salt of claim 2.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-01-22 | 1988-07-23 | 1989-01-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-01-22 | 1992-07-23 | 1993-01-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-01-22 | 1996-07-23 | 1997-01-22 | $7700.00 | Window Closed / Lapsed |