Abstract A process for purifying Mullerian Inhibiting Substance (MIS) from testicular tissue which comprises incubating testicular tissue in a culture media in the presence of a protease inhibitor in order to extract MIS from the tissue, chromatographing the extract on an anionic exchange resin followed by chromatographing the biologically active product obtained therefrom on a cationic exchange resin; the biologically active material from the cationic exchange resin is chromatographed by lectin affinity chromatography on wheat germ lectin and then either chromatographed by lectin affinity chromatography on concanavalin A or by triazinyl dye affinity chromatography on a appropriate triazinyl dye; MIS is obtained in 8,000-fold and 15,000-fold purification over original testicular tissue.
A composition comprising Mullerian Inhibiting Substance (MIS) derived from testicular tissue having an activity of at least 1 as measured by the Mullerian organ regression test being substantially free of albumen and being about 7,000-8,000-fold enriched in MIS activity over natural testicular tissue per dry weight of tissue.
Claim 2
Dependent Claim
48 words
A composition comprising Mullerian Inhibiting Substance (MIS) derived from testicular tissue having an activity of at least 1 as measured by the Mullerian organ regression test being substantially free of albumen and being about 7,000-8,000-fold enriched in MIS activity over natural testicular tissue per dry weight of tissue.
Claim 3
Dependent Claim
62 words
A composition comprising Mullerian Inhibiting Substance (MIS) derived from testicular tissue having an activity of at least 2-2.5 as measured by the Mullerian regression organ test; being substantially free of albumen and of proteinaceous material having a molecular weight of 35,000±10,000 Daltons and 70,000±10,000 Daltons, being about 15,000-fold enriched in MIS activity over natural testicular tissue per dry weight of said tissue.
Claim 4
Dependent Claim
62 words
A composition comprising Mullerian Inhibiting Substance (MIS) derived from testicular tissue having an activity of at least 2-2.5 as measured by the Mullerian regression organ test; being substantially free of albumen and of proteinaceous material having a molecular weight of 35,000±10,000 Daltons and 70,000±10,000 Daltons, being about 15,000-fold enriched in MIS activity over natural testicular tissue per dry weight of said tissue.
Claim 5
Independent Claim
32 words
A method of suppressing the growth of human ovarian cancer cells which comprises contacting said cells with a growth suppressing amount of the MIS composition of any of claims 1 or 2.