Chemotherapeutic agent and tracer composition and use thereof

Claim 1

A therapeutic and detecting composition suitable for extracorporeal treatment of whole blood comprising a water-soluble chemotherapeutic agent for sickle cell anemia said agent being selected from the group consisting of a water-soluble cyanate containing a reactive --NCO group, an alkyl acetimidate, and an alkyl adipimate, and a water-soluble fluorescable tracer means, said tracer being selected from the group consisting of an N-substituted benzamide represented by the formula ##STR2## wherein R is a member of the group consisting of hydrogen and methyl, X is a member of the group consisting of halogen and alkoxy having no more than two carbon atoms, and n is an integer having a value of 0 or 1, and physiologically tolerable alkali metal salts thereof said chemotherapeutic agent and said tracer means being dialyzable from whole blood at rates that are a function of one another.

Claim 13

A method for determining the concentration of dialyzable chemotherapeutic agent for sickle cell anemia said agent being selected from the group consisting of a water-soluble cyanate containing a reactive --NCO group, an alkyl acetimidate, and an alkyl adipimate, in an extracorporeal stream of whole blood containing said chemotherapeutic agent and salicylamide which comprises the steps of: contacting at least a portion of said extracorporeal stream across a dialysis membrane with an aqueous alkaline solution devoid of salicylamide for a predetermined time period; thereafter recovering said aqueous alkaline solution; irradiating an aliquot of the recovered aqueous alkaline solution with ultraviolet radiation haveing a wavelength of about 318 nanometers; positioning the irradiated aliquot adjacent to a detector means generating an output signal in response to emitted radiation having a wavelength of about 410 nanometers, said signal being proportional to the intensity of the emitted radiation; energizing the indicator means with said output signal and determining the concentration of remaining indicator wherein the concentration of remaining indicator is an indication of the concentration of the dialyzable chemotherapeutic agent present in the sample.

Claim 14

A method for determining the concentration of dialyzable chemotherapeutic agent for sickle cell anemia said agent being selected from the group consisting of a water-soluble cyanate containing a reactive --NCO group, an alkyl acetimidate, and an alkyl adipimate, in an extracorporeal stream of whole blood containing said chemotherapeutic agent and salicylamide which comprises the steps of: contacting at least a portion of said extracorporeal stream across a dialysis membrane with an aqueous alkaline solution devoid of salicylamide for a predetermined time period; thereafter recovering said aqueous alkaline solution; irradiating an aliquot of the recovered aqueous alkaline solution with ultraviolet radiation haveing a wavelength of about 318 nanometers; positioning the irradiated aliquot adjacent to a detector means generating an output signal in response to emitted radiation having a wavelength of about 410 nanometers, said signal being proportional to the intensity of the emitted radiation; energizing the indicator means with said output signal and determining the concentration of remaining indicator wherein the concentration of remaining indicator is an indication of the concentration of the dialyzable chemotherapeutic agent present in the sample.