Abstract This invention relates to an antibody capable of both specifically detecting and quantifying minute quantities of the herbicide, atrazine, left in biological samples, such antibody having been made by the process of first substituting a soluble straight chain amino acid at the 4 or 6 position of 2-chloro-4(isopropylamino)-6-(ethylamino)-S-atrazine so as to leave the chlorine exposed at the 2 position along with one of the amino groups, then conjugating the resulting substitution product at the site of the amino acid group to a lysine-rich protein, using the mixed anhydride method in a strongly-basic aqueous solution, then inoculating a susceptible host with the antigen to evoke an immune response, and thereafter harvesting the antibody.
The antibody for the immunoassay of atrazine made by the process of first derivatizing 2-chloro-4(isopropylamino)-6-(ethylamino)-S-atrazine by substituting a soluble straight chain amino acid having at least four carbon atoms at either the 4 or the 6 position so as to leave the chlorine exposed at the 2 position and one of the amino groups exposed at the 4 or the 6 position, then conjugating the resulting derivative at the site of the amino acid substitution to a lysine-rich protein using the mixed anhydride method in a strongly-basic aqueous solution to produce the antigen, next inoculating a susceptible host with the antigen to evoke an immune response and thereafter harvesting the antibody from the host.
Claim 2
Dependent Claim
114 words
The antibody for the immunoassay of atrazine made by the process of first derivatizing 2-chloro-4(isopropylamino)-6-(ethylamino)-S-atrazine by substituting a soluble straight chain amino acid having at least four carbon atoms at either the 4 or the 6 position so as to leave the chlorine exposed at the 2 position and one of the amino groups exposed at the 4 or the 6 position, then conjugating the resulting derivative at the site of the amino acid substitution to a lysine-rich protein using the mixed anhydride method in a strongly-basic aqueous solution to produce the antigen, next inoculating a susceptible host with the antigen to evoke an immune response and thereafter harvesting the antibody from the host.
Claim 3
Dependent Claim
23 words
The antibody made in accordance with the process of claim 1 wherein: the amino acid contains between four and approximately fourteen carbon atoms.
Claim 4
Dependent Claim
27 words
The antibody made in accordance with the process of claim 1 wherein: the lysine-rich protein is selected from the group consisting of bovine serum albumin and ovalbumin.