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US4560459A Expired / Lapsed US ⏱️ 0.0 years remaining

Vertical gel sandwich for use in electrophoresis and method therefor

Assignee: Bank of America NA
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Filing: 1984-02-21
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Granted: 1985-12-24
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Expires: 2004-02-21
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Abstract

Abstract A vertical gel sandwich for use in electrophoresis and a method of assembling is provided by the use of a pair of T-shaped spacers between the two plates of a gel sandwich. The stem of the T spaces the plates apart to form the gel slot and the head of the T being flush with the sides of both plates provides for alignment of the spacers and also prevents shifting.

Inventors

  • 👤 Peter S. Hoefer

Bibliographic Details

Application Number —
Jurisdiction US
Data Source google_patents
Last Synchronized 2026-10-28 23:09
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; G01N27/44704

Claims Analysis

Total Claims: 7
Claim 1
Independent Claim 152 words
A method of assembling a gel sandwich for use in electrophoresis comprising the following steps: laying down one plate of a pair; placing a pair of elongated straight T-shaped spacers with stems having planar parallel faces on opposite sides of said plate with the head of each T spacer flush against the side of the plate and the face of the stem of said T spacer being contiguous with said plate; placing the other plate of said pair on said spacers with the sides of such plate flush against the respective heads of said T spacers and with the faces of the respective stems contiguous to the plates whereby a sandwich is formed and having a slot and the top and bottom of said gel slot remain open to allow application of a voltage to a gel in said slot for electrophoretic separation; and pouring gel into said slot in said sandwich.
Claim 2
Independent Claim 152 words
A method of assembling a gel sandwich for use in electrophoresis comprising the following steps: laying down one plate of a pair; placing a pair of elongated straight T-shaped spacers with stems having planar parallel faces on opposite sides of said plate with the head of each T spacer flush against the side of the plate and the face of the stem of said T spacer being contiguous with said plate; placing the other plate of said pair on said spacers with the sides of such plate flush against the respective heads of said T spacers and with the faces of the respective stems contiguous to the plates whereby a sandwich is formed and having a slot and the top and bottom of said gel slot remain open to allow application of a voltage to a gel in said slot for electrophoretic separation; and pouring gel into said slot in said sandwich.
Claim 3
Dependent Claim 31 words
A method as in claim 1 where a casting container is provided including the step of vertically orienting a plurality of said sandwiches in said container for facilitating said gel pouring.
Claim 4
Dependent Claim 140 words
A vertical gel sandwich for use in electrophoresis comprising: a pair of juxtaposed flat plates with gel therebetween; means for spacing said plates apart and parallel with each other to form a gel slot including a pair of straight T-shaped spacers, the stem of the T having planar parallel faces and being inserted between said plates providing a pair of side boundaries for said gel slot and the top and bottom of said gel slot remain open to allow application of a voltage to said gel for elecrtrophoretic separation, the head of the T fitting flush against the sided of said pair of plates and the parallel faces of the stem of said T spacer being contiguous with said plates to thereby align its stem portion parallel to said sides of such plates and to prevent shifting of said spacer.
Claim 5
Dependent Claim 140 words
A vertical gel sandwich for use in electrophoresis comprising: a pair of juxtaposed flat plates with gel therebetween; means for spacing said plates apart and parallel with each other to form a gel slot including a pair of straight T-shaped spacers, the stem of the T having planar parallel faces and being inserted between said plates providing a pair of side boundaries for said gel slot and the top and bottom of said gel slot remain open to allow application of a voltage to said gel for elecrtrophoretic separation, the head of the T fitting flush against the sided of said pair of plates and the parallel faces of the stem of said T spacer being contiguous with said plates to thereby align its stem portion parallel to said sides of such plates and to prevent shifting of said spacer.
Claim 6
Dependent Claim 32 words
A gel sandiwch as in claim 3 where said sides of said plates have a minimum predetermined thickness said head of said T-spacer having a corresponding dimension no greater than said thickness.
Claim 7
Dependent Claim 33 words
A gel sandwich as in claim 3 togetehr with a casting container for retaining a plurality of said sandwiches in a vertical orientation to allow the pouring of gel into said gel slots.

References Cited (Backward Citations)

US3881683A Cited
US4035377A Cited
US4325796A Cited
US4279401A Cited

USPTO Annuity & Maintenance Windows

Annuity Stage Window Opens Payment Deadline Surcharge Period Until Estimated Fee Status
3.5 Years (Year 4) 1988-12-23 1989-06-24 1989-12-24 $2000.00 Window Closed / Lapsed
7.5 Years (Year 8) 1992-12-23 1993-06-24 1993-12-24 $3760.00 Window Closed / Lapsed
11.5 Years (Year 12) 1996-12-23 1997-06-24 1997-12-24 $7700.00 Window Closed / Lapsed

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