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Patent US4563422A successfully refreshed from Google Patents.
US4563422A
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US
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Method and composition for the removal of ascorbic acid from aqueous liquids
Assignee: Roche Diagnostics GmbH
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Filing: 1982-05-21
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Granted: 1986-01-07
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Expires: 2002-05-21
Abstract
Abstract The present invention provides a process for removing ascorbic acid from aqueous solutions by the addition of ascorbate oxidase, wherein catalase and hydrogen peroxide are also added to the solution. The present invention also provides an agent for the removal of ascorbic acid from aqueous solutions, based on a solid carrier material impregnated with ascorbate oxidase, wherein the carrier material also contains catalase and hydrogen peroxide and optionally also a stabilizer and/or buffer substance.
Inventors
- 👤 Ulfert Deneke
- 👤 Hans Lange
- 👤 Walter Rittersdorf
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 22:00
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/54; C12Q1/26; Y; Y10; Y10S; Y10S435/00; Y10S435/805; Y10S435/81; Y10T; Y10T436/00; Y10T436/14; Y10T436/142222; Y10T436/25; Y10T436/25125
Claims Analysis
Total Claims: 21
Claim 1
Independent Claim
39 words
Process for removing ascorbic acid from an aqueous solution containing same which process comprises adding to the aqueous solution, ascorbate oxidase, catalase and hydrogen peroxide, the catalase being added simultaneously with, or prior to the addition of hydrogen peroxide.
Claim 2
Dependent Claim
39 words
Process for removing ascorbic acid from an aqueous solution containing same which process comprises adding to the aqueous solution, ascorbate oxidase, catalase and hydrogen peroxide, the catalase being added simultaneously with, or prior to the addition of hydrogen peroxide.
Claim 3
Dependent Claim
20 words
Process as claimed in claim 1 wherein the hydrogen peroxide is used in the form of a hydrogen peroxide-containing adduct.
Claim 4
Dependent Claim
12 words
Process as claimed in claim 2 wherein said adduct is a perborate.
Claim 5
Dependent Claim
13 words
Process as claimed in claim 2 wherein said adduct is a urea perhydrate.
Claim 6
Dependent Claim
13 words
Process as claimed in claim 2 wherein said adduct is a mannitol perhydrate.
Claim 7
Dependent Claim
29 words
Reagent for the removal of ascorbic acid from aqueous solutions comprising a solid carrier material impregnated with ascorbate oxidase, wherein the carrier material also contains catalase and hydrogen peroxide.
Claim 8
Dependent Claim
29 words
Reagent for the removal of ascorbic acid from aqueous solutions comprising a solid carrier material impregnated with ascorbate oxidase, wherein the carrier material also contains catalase and hydrogen peroxide.
Claim 9
Dependent Claim
14 words
Reagent as claimed in claim 6 wherein said carrier material additionally contains a stabilizer.
Claim 10
Dependent Claim
17 words
Reagent as claimed in claim 7 wherein said stabilizer is pectin, polyvinylpyrrolidone or dextran, or mixtures thereof.
Claim 11
Dependent Claim
15 words
Reagent as claimed in claim 6 wherein said carrier material additionally contains a buffer substance.
Claim 12
Dependent Claim
25 words
Reagent as claimed in claim 6 wherein said carrier material contains the ascorbate oxidase and the catalase together and the hydrogen peroxide physically separated therefrom.
Claim 13
Dependent Claim
16 words
Reagent as claimed in claim 6 wherein said hydrogen peroxide is present as a solid adduct.
Claim 14
Dependent Claim
40 words
Reagent as claimed in claim 6 wherein said carrier material consists of the first compact carrier material to which is fixed at least one second porous absorbent carrier material which is impregnated with one or more of the active materials.
Claim 15
Dependent Claim
20 words
Reagent as claimed in claim 12 wherein the first carrier material is in the form of a strip or rod.
Claim 16
Dependent Claim
17 words
Reagent as claimed in claim 12 wherein the first carrier material consists of glass or synthetic resin.
Claim 17
Dependent Claim
16 words
Reagent as claimed in claim 12 wherein the second carrier material is a paper or fleece.
Claim 18
Dependent Claim
26 words
Reagent as claimed in claim 6 wherein said carrier material contains a buffer substance which is effective at a pH value of from 5 to 8.5.
Claim 19
Dependent Claim
16 words
Reagent as claimed in claim 16 wherein said buffer substance is tris-citrate or tris-citric acid buffer.
Claim 20
Dependent Claim
14 words
Reagent as claimed in claim 16 containing at least 30 U of ascorbate oxidase.
Claim 21
Dependent Claim
14 words
Reagent as claimed in claim 18 containing 35 to 40 U of ascorbate oxidase.
References Cited (Backward Citations)
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1989-01-06 | 1989-07-08 | 1990-01-07 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1993-01-06 | 1993-07-08 | 1994-01-07 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1997-01-06 | 1997-07-08 | 1998-01-07 | $7700.00 | Window Closed / Lapsed |