Patent US626198A successfully refreshed from Google Patents.
US4575488A
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Interference free transaminase assay
Assignee: Bayer Corp
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Filing: 1982-06-25
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Granted: 1986-03-11
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Expires: 2002-06-25
Abstract
Abstract Compositions for pyridoxal-5'-phosphate (PLP) activated transaminase determinations are provided, in which interference due to PLP is avoided. Amino-functionalized polymers bind PLP which is not associated with the apotransaminase in the sample to be tested, thereby avoiding signal interference, e.g. background absorbance, due to PLP.
Inventors
- 👤 Jan S. Krouwer
- 👤 Michael J. Lynch
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 10:02
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/48; C12Q1/52; Y; Y10; Y10S; Y10S435/00; Y10S435/81
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
93 words
A composition for the determination of a transaminase in a sample, which composition comprises: (a) a substrate for said transaminase; (b) a reagent responsive to the reaction product of said transaminase and said substrate to provide a detectable response; (c) pyridoxal-5'-phosphate; and (d) an amino functionalized polymer having the formula ##STR7## wherein R 1 is hydrogen, alkyl or aryl; R 2 and R 3 are independently hydrogen, alkyl or aryl; and n is the number of repeating units of the polymer in an amount sufficient to remove the signal interference caused by pyridoxal-5'-phosphate.
Claim 2
Dependent Claim
93 words
A composition for the determination of a transaminase in a sample, which composition comprises: (a) a substrate for said transaminase; (b) a reagent responsive to the reaction product of said transaminase and said substrate to provide a detectable response; (c) pyridoxal-5'-phosphate; and (d) an amino functionalized polymer having the formula ##STR7## wherein R 1 is hydrogen, alkyl or aryl; R 2 and R 3 are independently hydrogen, alkyl or aryl; and n is the number of repeating units of the polymer in an amount sufficient to remove the signal interference caused by pyridoxal-5'-phosphate.
Claim 3
Dependent Claim
28 words
The composition of claim 1 wherein the reagent of (b) comprises an enzyme for which the reaction product is a substrate and a redox co-factor for the enzyme.
Claim 4
Dependent Claim
36 words
The composition of claim 2 wherein the transaminase is glutamate oxalacetate transaminase, the substrate comprises aspartate and α-ketoglutarate, the enzyme specific for the reaction product comprises malate dehydrogenase and the redox coenzyme is nicotine adenine dinucleotide.
Claim 5
Dependent Claim
36 words
The composition of claim 2 wherein the transaminase is glutamate pyruvate transaminase, the substrate comprises alanine and α-ketoglutarate, the enzyme specific for the reaction product is lactate dehydrogenase and the redox coenzyme is nicotine adenine dinucleotide.
Claim 6
Dependent Claim
16 words
The composition of claim 2 wherein the enzyme specific for the reaction product comprises glutamate dehydrogenase.
Claim 7
Dependent Claim
37 words
The composition of claim 1 wherein the transaminase is glutamate oxalacetate transaminase, the substrate comprises glutamate and oxalacetate, the reagent responsive to the reaction product comprises γ-aminobutyrate, γ-aminobutyrate transaminase, succinate semialdehyde dehydrogenase and nicotine adenine dinucleotide phosphate.
Claim 8
Dependent Claim
37 words
The composition of claim 1 wherein the transaminase is glutamate pyruvate transaminase, the substrate comprises glutamate and pyruvate, the reagent responsive to the reaction product comprises γ-aminobutyrate, γ-aminobutyrate transaminase, succinate semialdehyde dehydrogenase and nicotine adenine dinucleotide phosphate.
Claim 9
Dependent Claim
13 words
The composition of any of claims 1-7 which further comprises a redox indicator.
Claim 10
Dependent Claim
17 words
The composition of claim 1 wherein R 1 , R 2 and R 3 are each hydrogen.
Claim 11
Dependent Claim
24 words
The composition of claim 1 wherein R 1 , R 2 and R 3 are each hydrogen and n is at least about 100.
Claim 12
Dependent Claim
21 words
The composition of claim 1 wherein R 1 is methyl, ethyl or benzyl and R 2 and R 3 are hydrogen.
Claim 13
Dependent Claim
19 words
The composition of claim 1 wherein R 1 and R 3 are both ethyl and R 2 is hydrogen.
Claim 14
Dependent Claim
29 words
The composition of claim 1 wherein one of R 2 and R 3 is hydroxyl and R 1 and the other of R 2 and R 3 is hydrogen.
Claim 15
Dependent Claim
20 words
The composition of claim 1 wherein R 1 is hydrogen and both of R 2 and R 3 are methyl.
Claim 16
Independent Claim
73 words
A method for determining a transaminase in a liquid, which method comprises contacting said liquid with a composition which comprises a substrate for the transaminase, a reagent responsive to the reaction product of the transaminase and the substrate, and pyridoxal-5'-phosphate to form a reaction mixture; thereafter, contacting said reaction mixture with an amino-functionalized polymer; separating from said liquid any pyridoxal-5'-phosphate bound to amino-functionalized polymer; and, thereafter, observing any detectable response in said liquid.
Claim 17
Independent Claim
73 words
A method for determining a transaminase in a liquid, which method comprises contacting said liquid with a composition which comprises a substrate for the transaminase, a reagent responsive to the reaction product of the transaminase and the substrate, and pyridoxal-5'-phosphate to form a reaction mixture; thereafter, contacting said reaction mixture with an amino-functionalized polymer; separating from said liquid any pyridoxal-5'-phosphate bound to amino-functionalized polymer; and, thereafter, observing any detectable response in said liquid.
References Cited (Backward Citations)
US3458403A
Cited
US4372874A
Cited
US4271265A
Cited
US4241179A
Cited
US4235962A
Cited
JPS568691A
Cited
US4450232A
Cited
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