Patent US4596768A successfully refreshed from Google Patents.
US4596768A
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Antibodies for penicilloic acid their preparation and use
Assignee: Siemens Healthcare Diagnostics GmbH Germany
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Filing: 1983-11-02
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Granted: 1986-06-24
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Expires: 2003-11-02
Abstract
Abstract Conjugates of penicilloic acid derivatives and certain poly(amino acids), which are either antigenic or enzymatic, are provided. Antibodies raised against the antigenic poly(amino acids) and the enzyme conjugates are used as reagents in immunoassays. In particular, the compounds of the present invention can be used for measuring the presence of a beta -lactamase in a patient serum sample by adding a known amount of penicillin to the sample and observing the production of penicilloic acid over a fixed period of time.
Inventors
- 👤 Prithipal Singh
- 👤 Danton K. Leung
- 👤 Richard Rodgers
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 18:20
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/94; G01N33/9446; G01N33/53; G01N33/531; G01N33/532; G01N33/535; Y; Y10; Y10S; Y10S435/00; Y10S435/805; Y10S435/81; Y10S435/961; Y10S435/964; Y10S436/00; Y10S436/807; Y10S436/81; Y10S436/815; Y10S436/822; Y10S530/00; Y10S530/807
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
70 words
A compound of the formula: ##STR3## wherein: X is an alkali metal or hydrogen; Y is an organic radical of naturally occurring or synthetic penicillin antibiotics; R is a linking group having from 3 to 9 carbon atoms and from 0 to 5 heteroatoms; Z is a poly(amino acid) which is an antigen or an enzyme; and n is from 1 to the molecular weight of Z divided by 500.
Claim 2
Dependent Claim
70 words
A compound of the formula: ##STR3## wherein: X is an alkali metal or hydrogen; Y is an organic radical of naturally occurring or synthetic penicillin antibiotics; R is a linking group having from 3 to 9 carbon atoms and from 0 to 5 heteroatoms; Z is a poly(amino acid) which is an antigen or an enzyme; and n is from 1 to the molecular weight of Z divided by 500.
Claim 3
Dependent Claim
21 words
A compound as in claim 1, wherein R is a straight chain having from 5 to 12 atoms in the chain.
Claim 4
Dependent Claim
10 words
A compound as in claim 2, where R is: ##STR4##
Claim 5
Dependent Claim
10 words
A compound as in claim 1, wherein Y is phenyl.
Claim 6
Dependent Claim
11 words
A compound as in claim 1, wherein Z is an antigen.
Claim 7
Dependent Claim
16 words
A compound as in claim 1, wherein Z is a bovine serum albumin or bovine gamma-globulin.
Claim 8
Dependent Claim
11 words
A compound as in claim 1, wherein Z is an enzyme.
Claim 9
Dependent Claim
11 words
A compound as in claim 1, wherein Z is horseradish peroxidase.
Claim 10
Dependent Claim
129 words
A method for determining the presence of or amount of a penicilloic acid in a sample which comprises: combining in an assay medium a sample suspected of containing a penicilloic acid, enzyme-penicilloic acid conjugate and a solid support to which antibodies to said penicilloic acid are bound wherein said antibodies are obtained in response to an immunogen and wherein said immunogen and said enzyme-penicilloic acid conjugate are compounds according to claim 1, whereby said conjugate binds to said antibodies in inverse proportion to the amount of said penicilloic acid in said sample; separating said support from said assay medium; and determining the enzyme activity on said support or in said assay medium as an indication of the presence of or the amount of said penicilloic acid in said sample.
Claim 11
Dependent Claim
188 words
A method for determining the presence of or amount of a penicilloic acid in a sample which comprises: combining in an assay medium a sample suspected of containing a penicilloic acid, enzyme-penicilloic acid conjugate and a solid support to which antibodies are bound wherein said antibodies are obtained in response to an antigen according to claim 6, and wherein said enzyme-penicilloic acid conjugate is a compound of the formula: ##STR5## wherein: X is an alkali metal or hydrogen; Y is an organic radical of naturally occurring or synthetic penicillin antibiotics; R is a linking group having from 3 to 9 carbon atoms and from 0 to 5 heteroatoms; Z is a poly(amino acid) which is an enzyme; and n is from 1 to the molecular weight of Z divided by 500, whereby said conjugate binds to said antibodies in inverse proportion to the amount of said penicilloic acid in said sample; separating said support from said assay medium; and determining the enzyme activity on said support or in said assay medium as in indication of the presence of or the amount of said penicilloic acid in said sample.
Claim 12
Dependent Claim
123 words
A method for determining the presence of or amount of a penicilloic acid in a sample which comprises: combining in an assay medium, a sample suspected of containing a penicilloic acid, antibodies to said penicilloic acid, wherein said antibodies are obtained in response to an immunogen, and said penicilloic acid conjugated to an enzyme, wherein said immunogen and said enzyme-penicilloic acid conjugate are compounds according to claim 1, under conditions where said penicilloic acid conjugate can be distinguished between being bound to antibodies and being unbound, the amount bound being an indication of the amount of penicilloic acid in said sample; and determining either the amount of bound or unbound conjugate as an indication of the amount of penicilloic acid in said sample.
Claim 13
Dependent Claim
27 words
A method according to claim 11, wherein said sample is formed by combining a sample suspected of having β-lactamase with a penicillin precursor to said penicilloic acid.
Claim 14
Dependent Claim
13 words
A method according to claim 11, wherein said enzyme is horse radish peroxidase.
References Cited (Backward Citations)
US4234683A
Cited
US4347312A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1989-06-23 | 1989-12-23 | 1990-06-24 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1993-06-23 | 1993-12-23 | 1994-06-24 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1997-06-23 | 1997-12-23 | 1998-06-24 | $7700.00 | Window Closed / Lapsed |