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US4675113A
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Affinity chromatography using dried calcium alginate-magnetite separation media in a magnetically stabilized fluidized bed
Assignee: University Patents Inc
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Filing: 1986-09-12
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Granted: 1987-06-23
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Expires: 2006-09-12
Abstract
Abstract Disclosed is a method of conducting affinity chromatographic separations of biomaterials which comprises contacting the biomaterial with a solid support comprising a generally spherical bead having a generally central magnetic core and a surrounding exterior coat about the core comprising a material capable of binding with the biomaterial.
Inventors
- 👤 David J. Graves
- 👤 Mark A. Burns
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 22:33
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/76; C07K14/765; B; B01; B01D; B01D15/00; B01D15/08; B01D15/10; B01D15/18; B01D15/1807; B01D15/26; B01D15/38; B01D15/3804; B01J; B01J20/00; B01J20/02; B01J20/0203; B01J20/0225; B01J20/0229; B01J20/06; B01J20/28; B01J20/28002; B01J20/28004; B01J20/28009; B01J20/28011; B01J20/28014; B01J20/28016; B01J20/28019; B01J20/281; B01J20/286; B01J20/30; B01J20/3092; B01J20/32; B01J20/3202; B01J20/3204; B01J20/3231; B01J20/3242; B01J20/3244; B01J20/3246; B01J20/3248; B01J20/3251; B01J20/3253; B01J20/3255; B01J20/3268; B01J20/3272; B01J20/3274; B01J20/328; B01J20/3291; B01J20/3293; C12; C12N; C12N11/00; C12N11/02; C12N11/10; C12N11/14; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/551; G01N33/553; B01J2220/00; B01J2220/40; B01J2220/48; B01J2220/4812; B01J2220/4843; B01J2220/49; B01J2220/50; B01J2220/54; B01J2220/56; B01J2220/58
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
46 words
A method of conducting affinity chromatographic separations of biomaterials which comprises contacting the biomaterial with a solid support comprising a generally spherical bead having a generally central magnetic core, and a surrounding exterior coat about the core comprising a material capable of binding with the biomaterial.
Claim 2
Independent Claim
46 words
A method of conducting affinity chromatographic separations of biomaterials which comprises contacting the biomaterial with a solid support comprising a generally spherical bead having a generally central magnetic core, and a surrounding exterior coat about the core comprising a material capable of binding with the biomaterial.
Claim 3
Dependent Claim
15 words
A method according to claim 1 wherein the biomaterial is in a liquid carrier fluid.
Claim 4
Dependent Claim
13 words
A method according to claim 1 wherein the surrounding exterior coat is alginate.
Claim 5
Dependent Claim
12 words
A method according to claim 1 wherein the interior core is magnetite.
Claim 6
Dependent Claim
15 words
A method according to claim 1 wherein the exterior coat further comprises a binding ligand.
Claim 7
Dependent Claim
13 words
The method according to claim 5 wherein the ligand is a triazine dye.
Claim 8
Dependent Claim
12 words
The method according to claim 5 wherein the ligand is an antibody.
Claim 9
Dependent Claim
15 words
A method according to claim 2 wherein the solid support is within a support bed.
Claim 10
Dependent Claim
12 words
A method according to claim 8 wherein the support bed is fluidized.
Claim 11
Dependent Claim
15 words
A method according to claim 8 wherein the bed is a magnetically stabilized fluidized bed.
Claim 12
Dependent Claim
23 words
A method in accordance with claim 8 wherein the support material in said bed is held in place by a uniform magnetic field.
Claim 13
Dependent Claim
14 words
A method in accordance with claim 8 wherein the bed is a vertically-oriented bed.
Claim 14
Dependent Claim
42 words
A method in accordance with claim 12 which further comprises adding (1) said support material to said bed in admixture with an inert solvent, and (2) a feed stream containing a sample material to be separated, at the top of said bed.
Claim 15
Dependent Claim
18 words
A method in accordance with claim 13 which further comprises moving said support material downwardly through said bed.
Claim 16
Dependent Claim
41 words
A method in accordance with claim 14 which further comprises removing said support material from the bottom of said bed, and stripping any substances which have become bound to said support material from said feed stream off of said support material.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-06-22 | 1990-12-22 | 1991-06-23 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-06-22 | 1994-12-22 | 1995-06-23 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-06-22 | 1998-12-22 | 1999-06-23 | $7700.00 | Window Closed / Lapsed |