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US4684615A
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Stabilized isoenzyme control products
Assignee: Bayer Corp
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Filing: 1986-09-29
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Granted: 1987-08-04
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Expires: 2006-09-29
Abstract
Abstract Isoenzyme control reagents and methods for making same stabilized by means of plexiform stabilizing means. The preferred plexiform stabilizing means is selected from the group consisting of monosaccharide and disaccharide reducing sugars. The most preferred isoenzyme control reagent comprises the isoenzyme of interest obtained from selected tissue, preclarified human sera, stabilizing cofactor, a chelating agent means, a weak nonphosphate buffer and lactose as the most preferred plexiform stabilizing means.
Inventors
- 👤 Michael K. Hoskins
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 05:20
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/96; C; C12; C12Q; C12Q1/00; C12Q1/26; C12Q1/32; C12Q1/34; C12Q1/37; C12Q1/48; G01N33/74; G01N2496/00; G01N2496/05; G01N2496/80; Y; Y10; Y10T; Y10T436/00; Y10T436/10; Y10T436/106664; Y10T436/108331
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
59 words
A method of stabilizing an isoenzyme control reagent comprising adding to an aqueous solution containing the isoenzymes to be stabilized against disassociation or rearrangement of subunits a plexiform stabilizing means selected from the group consisting of reducing monosaccharide sugars and reducing disaccharide sugars, said plexiform stabilizing means present in the range of about 2% to 8% weight per volume.
Claim 2
Independent Claim
59 words
A method of stabilizing an isoenzyme control reagent comprising adding to an aqueous solution containing the isoenzymes to be stabilized against disassociation or rearrangement of subunits a plexiform stabilizing means selected from the group consisting of reducing monosaccharide sugars and reducing disaccharide sugars, said plexiform stabilizing means present in the range of about 2% to 8% weight per volume.
Claim 3
Dependent Claim
24 words
The method as provided in claim 1 wherein the plexiform stabilizing means is selected from the group consisting of maltose, mannitol, cellobiose and lactose.
Claim 4
Dependent Claim
14 words
The method as provided in claim 1 wherein the plexiform stabilizing means is lactose.
Claim 5
Dependent Claim
19 words
The method as provided in claim 3 wherein the lactose is present in the final concentration of about 6%.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-08-03 | 1991-02-02 | 1991-08-04 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-08-03 | 1995-02-02 | 1995-08-04 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-08-03 | 1999-02-02 | 1999-08-04 | $7700.00 | Window Closed / Lapsed |