US4724146A
Expired / Lapsed
US
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Method for preparation herpes simplex virus subunit vaccine
Assignee: Chemo Sero Therapeutic Research Institute Kaketsuken
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Filing: 1985-08-23
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Granted: 1988-02-09
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Expires: 2005-08-23
Abstract
Abstract A method for the preparation of an herpes simplex virus subunit vaccine, which comprises subjecting a solution containing glycoproteins gA and gB to column chromatography using, as a gel for chromatography, sulfuric acid ester of cellulose or a crosslinked polysaccharide to adsorb the glycoproteins gA and gB onto the gel in the presence of an anionic surfactant or a nonanionic surfactant, eluting the adsorbed glycoproteins to obtain an eluate containing the glycoproteins, and subjecting the eluate to gel filtration to obtain a filtrate containing the glycoproteins.
Inventors
- 👤 Youichiro Kino
- 👤 Hiroshi Mizokami
- 👤 Tetsuo Kawahara
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 20:53
CPC Classifications
A; A61; A61K; A61K39/00; A61K39/12; A61K39/245; A61K39/25; C; C07; C07K; C07K14/00; C07K14/005; C12; C12N; C12N2710/00; C12N2710/00011; C12N2710/16011; C12N2710/16611; C12N2710/16622; Y; Y10; Y10S; Y10S530/00; Y10S530/82; Y10S530/826
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
109 words
A method for the preparation of a herpes simplex virus subunit vaccine, which comprises subjecting a solution containing glycoproteins gA and gB to column chromatography, using as a gel for chromatography, a sulfuric acid ester of cellulose or a crosslinked polysaccharide to adsorb the glycoproteins gA and gB onto the gel in the presence of an anionic surfactant or nonionic surfactant, said sulfuric acid ester being prepared by treating a gel of cellulose or crosslinked polysaccharide with a sulfating agent in an organic solvent, eluting the adsorbed glycoproteins to obtain an eluate containing the glycoproteins, and subjecting the eluate to gel filtration to obtain a filtrate containing the glycoproteins.
Claim 2
Independent Claim
109 words
A method for the preparation of a herpes simplex virus subunit vaccine, which comprises subjecting a solution containing glycoproteins gA and gB to column chromatography, using as a gel for chromatography, a sulfuric acid ester of cellulose or a crosslinked polysaccharide to adsorb the glycoproteins gA and gB onto the gel in the presence of an anionic surfactant or nonionic surfactant, said sulfuric acid ester being prepared by treating a gel of cellulose or crosslinked polysaccharide with a sulfating agent in an organic solvent, eluting the adsorbed glycoproteins to obtain an eluate containing the glycoproteins, and subjecting the eluate to gel filtration to obtain a filtrate containing the glycoproteins.
Claim 3
Dependent Claim
23 words
The method as claimed in claim 1, wherein the glycoproteins-containing solution is obtained from culture cells infected with herpes simplex virus subunit vaccine.
Claim 4
Dependent Claim
35 words
The method as claimed in claim 1, wherein the sulfuric acid ester of crosslinked polysaccharide is selected from the group consisting of a crosslinked cellulose sulfate, a crosslinked agarose sulfate and a crosslinked dextran sulfate.
Claim 5
Dependent Claim
32 words
The method as claimed in claim 1, wherein the sulfuric acid ester of cellulose is a sulfuric acid ester of crystalline cellulose or a cellulose having a crystalline area and non-crystalline area.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-02-08 | 1991-08-10 | 1992-02-09 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-02-08 | 1995-08-10 | 1996-02-09 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-02-08 | 1999-08-10 | 2000-02-09 | $7700.00 | Window Closed / Lapsed |