US4737259A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Element for electrophoresis
Assignee: Fujifilm Holdings Corp
•
Filing: 1986-12-29
•
Granted: 1988-04-12
•
Expires: 2006-12-29
Abstract
Abstract An element for electrophoresis suitably employable for electrophoresis of biopolymers such as proteins, as well as for the determination of the base sequence of DNA, RNA, their fragments, and their derivatives, which comprises a plastic support, a nonconductive metal oxide layer, and an electrophoresis medium layer comprising an aqueous polyacrylamide gel formed by crosslinking polymerization of an acrylamide compound and a crosslinking agent in the presence of water, which are superposed in this order. The electrophoresis medium layer may contain a water-soluble polymer and agarose. The medium layer may contain a modifier such as an anionic surfactant, formamide or urea.
Inventors
- 👤 Masashi Ogawa
- 👤 Masakazu Hashiue
- 👤 Yuzo Mizobuchi
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 11:24
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; G01N27/44704; G01N27/44747; Y; Y10; Y10T; Y10T428/00; Y10T428/31504; Y10T428/31725; Y10T428/31765
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
49 words
An element for electrophoresis comprising in the following order: (I) a plastic material support layer; (II) a nonconductive oxide layer; and (III) a gel membrane for electrophoresis comprising an aqueous polyacrylamide gel formed by crosslinking polymerization of an acrylamide compound and a crosslinking agent in the presence of water.
Claim 2
Dependent Claim
49 words
An element for electrophoresis comprising in the following order: (I) a plastic material support layer; (II) a nonconductive oxide layer; and (III) a gel membrane for electrophoresis comprising an aqueous polyacrylamide gel formed by crosslinking polymerization of an acrylamide compound and a crosslinking agent in the presence of water.
Claim 3
Dependent Claim
47 words
The element for electrophoresis as claimed in claim 1, in which said nonconductive oxide layer is selected from the group consisting of SiO 2 , TiO 2 , MgO, Al 2 O 3 , BeO, ZrO 2 , WO 2 , and Ta 2 O 3 .
Claim 4
Dependent Claim
27 words
The element for electrophoresis as claimed in claim 2, in which said nonconductive oxide is selected from the group consisting of SiO 2 and TiO 2 .
Claim 5
Dependent Claim
24 words
The element for electrophoresis as claimed in claim 1, in which said nonconductive oxide layer is treated with a vinyl silane on its surface.
Claim 6
Dependent Claim
26 words
The element for electrophoresis as claimed in any one of claims 1 to 4, in which the plastic material support layer is a polyethylene terephthalate sheet.
Claim 7
Dependent Claim
25 words
The element for electrophoresis as claimed in any one of claims 1 to 4, in which said gel membrane contains a water-soluble polymer and agarose.
Claim 8
Dependent Claim
23 words
The element for electrophoresis as claimed in any one of claims 1 to 4, in which said gel membrane contains an anionic surfactant.
Claim 9
Dependent Claim
17 words
The element for electrophoresis as claimed in claim 7, in which said anionic surfactant is an alkylsulfate.
Claim 10
Dependent Claim
16 words
The element for electrophoresis as claimed in claim 8, in which said alkylsulfate is sodium dodecylsulfate.
Claim 11
Dependent Claim
28 words
The element for electrophoresis as claimed in any one of claims 1 to 4, in which said gel membrane contains a compound having at least one carbamoyl group.
Claim 12
Dependent Claim
23 words
The element for electrophoresis as claimed in claim 10, in which said compound having at least one carbamoyl group is urea or formamide.
References Cited (Backward Citations)
US3875044A
Cited
US3922432A
Cited
US3960499A
Cited
US4415428A
Cited
US4548870A
Cited
US4548869A
Cited
US4579783A
Cited
US4600641A
Cited
US4657656A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-04-12 | 1991-10-12 | 1992-04-12 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-04-12 | 1995-10-12 | 1996-04-12 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-04-12 | 1999-10-12 | 2000-04-12 | $7700.00 | Window Closed / Lapsed |