Patent US4752572A successfully refreshed from Google Patents.
US4752572A
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Lipid vesicles containing labeled species and their analytical uses
Assignee: Clinical Diagnostic Systems Inc
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Filing: 1985-08-30
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Granted: 1988-06-21
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Expires: 2005-08-30
Abstract
Abstract Vesicles comprising a matrix of lipid membranes prepared from lipid materials are useful in biomedical studies and immunoassays. A labeled species is encapsulated within the vesicles and released when the vesicles are lysed with a surface active agent. The outer surface of the vesicles is essentially free of the labeled species. Immunoassays can be carried out in solution or with a dry analytical element.
Inventors
- 👤 Michael W. Sundberg
- 👤 David F. O'Brien
- 👤 Susan J. Danielson
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 18:28
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/52; G01N33/525; G01N33/53; G01N33/531; G01N33/532; G01N33/543; G01N33/54366; G01N33/54386; G01N33/544; Y; Y10; Y10S; Y10S435/00; Y10S435/805; Y10S435/81; Y10S435/97; Y10S435/975; Y10S436/00; Y10S436/807; Y10S436/808; Y10S436/81; Y10S436/829; Y10T; Y10T428/00; Y10T428/29; Y10T428/2982; Y10T428/2984
Claims Analysis
Total Claims: 25
Claim 1
Independent Claim
43 words
An analytical composition for determining an immunologically active ligand comprising vesicles, said vesicles consisting essentially of lipid membranes with a labeled analog of said ligand encapsulated therein, and having an outer surface which is essentially free of said ligand and a receptor therefor.
Claim 2
Dependent Claim
43 words
An analytical composition for determining an immunologically active ligand comprising vesicles, said vesicles consisting essentially of lipid membranes with a labeled analog of said ligand encapsulated therein, and having an outer surface which is essentially free of said ligand and a receptor therefor.
Claim 3
Dependent Claim
13 words
The composition of claim 1 wherein said labeled ligand is an enzyme-labeled ligand.
Claim 4
Dependent Claim
13 words
The composition of claim 1 wherein said labeled ligand is a labeled antigen.
Claim 5
Dependent Claim
27 words
The composition of claim 1 wherein said lipid membranes are prepared from materials selected from the group consisting of phospholipids, sphingolipids, glycolipids, phosphonolipids, sterols and fatty acids.
Claim 6
Dependent Claim
34 words
The composition of claim 4 wherein said membrane material is a phospholipid represented by the structure: ##STR2## wherein R' and R" are independently divalent aliphatic groups, and X is an alkylene quaternary ammonium cation.
Claim 7
Dependent Claim
54 words
A dry analytical element for the determination of an immunologically active ligand, said element comprising an absorbent carrier material containing a composition comprising vesicles, said vesicles comprising lipid membranes with a labeled analog of said ligand encapsulated therein, and having an outer surface which is essentially free of said ligand and a receptor therefor.
Claim 8
Dependent Claim
54 words
A dry analytical element for the determination of an immunologically active ligand, said element comprising an absorbent carrier material containing a composition comprising vesicles, said vesicles comprising lipid membranes with a labeled analog of said ligand encapsulated therein, and having an outer surface which is essentially free of said ligand and a receptor therefor.
Claim 9
Dependent Claim
27 words
The element of claim 6 wherein said lipid membranes are prepared from materials selected from the group consisting of phospholipids, sphingolipids, glycolipids, phosphonolipids, sterols and fatty acids.
Claim 10
Dependent Claim
77 words
A dry analytical element for the determination of an immunologically active ligand comprising a support having thereon a porous spreading zone, and a composition comprising vesicles, said vesicles comprising phospholipid membranes represented by the structure: ##STR3## wherein R' and R" are independently divalent aliphatic groups, and X is an alkylene quaternary ammonium cation, said vesicles having a labeled analog of said ligand encapsulated therein, and an outer surface essentially free of said ligand and a receptor therefor.
Claim 11
Dependent Claim
77 words
A dry analytical element for the determination of an immunologically active ligand comprising a support having thereon a porous spreading zone, and a composition comprising vesicles, said vesicles comprising phospholipid membranes represented by the structure: ##STR3## wherein R' and R" are independently divalent aliphatic groups, and X is an alkylene quaternary ammonium cation, said vesicles having a labeled analog of said ligand encapsulated therein, and an outer surface essentially free of said ligand and a receptor therefor.
Claim 12
Dependent Claim
11 words
The element of claim 8 comprising a receptor for said ligand.
Claim 13
Dependent Claim
95 words
A dry multilayer analytical element for the determination of an antigen comprising a support having thereon, in order and in fluid contact, a registration layer comprising a colorimetric indicator composition and an enzyme substrate, an isotropically porous spreading layer comprising immobilized antibody for said antigen and vesicles comprising phospholipid membranes repre sented by the structure: ##STR4## wherein R' and R" are independently alkylene groups, and X is an alkylene quaternary ammonium cation, said vesicles having said antigen labeled with an enzyme encapsulated therein, and an outer surface essentially free of said antigen and said antibody.
Claim 14
Dependent Claim
95 words
A dry multilayer analytical element for the determination of an antigen comprising a support having thereon, in order and in fluid contact, a registration layer comprising a colorimetric indicator composition and an enzyme substrate, an isotropically porous spreading layer comprising immobilized antibody for said antigen and vesicles comprising phospholipid membranes repre sented by the structure: ##STR4## wherein R' and R" are independently alkylene groups, and X is an alkylene quaternary ammonium cation, said vesicles having said antigen labeled with an enzyme encapsulated therein, and an outer surface essentially free of said antigen and said antibody.
Claim 15
Independent Claim
82 words
A method for the determination of an immunologically active ligand in a liquid, said method comprising the steps of: A. in the presence of a receptor for said ligand, contacting a sample of said liquid with an analytical composition comprising vesicles, said vesicles comprising lipid membranes with a labeled analog of said ligand encapsulated therein, and having an outer surface essentially free of said ligand and receptor, B. lysing said vesicles, and C. determining said labeled analog in bound or unbound form.
Claim 16
Independent Claim
82 words
A method for the determination of an immunologically active ligand in a liquid, said method comprising the steps of: A. in the presence of a receptor for said ligand, contacting a sample of said liquid with an analytical composition comprising vesicles, said vesicles comprising lipid membranes with a labeled analog of said ligand encapsulated therein, and having an outer surface essentially free of said ligand and receptor, B. lysing said vesicles, and C. determining said labeled analog in bound or unbound form.
Claim 17
Dependent Claim
24 words
The method of claim 11 wherein said vesicles are lysed with a surfactant having a critical micelle concentration of at least about 0.1 mmolar.
Claim 18
Dependent Claim
27 words
The method of claim 11 wherein said lipid membranes are prepared from materials selected from the group consisting of phospholipids, sphingolipids, glycolipids, phosphonolipids, sterols and fatty acids.
Claim 19
Dependent Claim
34 words
The method of claim 13 wherein said membrane material is a phospholipid represented by the structure: ##STR5## wherein R' and R" are independently divalent aliphatic groups, and X is an alkylene quaternary ammonium cation.
Claim 20
Dependent Claim
16 words
The method of claim 11 wherein said analytical composition is contained in a dry analytical element.
Claim 21
Dependent Claim
14 words
The method of claim 15 wherein said element contains a receptor for said ligand.
Claim 22
Independent Claim
122 words
A method for the determination of an antigen in a biological fluid, said method comprising the steps of: A. in the presence of an antibody for said antigen, contacting a sample of said fluid with an analytical composition comprising vesicles, said vesicles comprising lipid membranes prepared from a phospholipid represented by the structure: ##STR6## wherein R' and R" are independently alkylene groups, and X is an alkylene quaternary ammonium cation, said vesicles having said antigen labeled with an enzyme encapsulated therein, and an outer surface essentially free of said antigen and said antibody, B. lysing said vesicles with a surfactant having a critical micelle concentration of at least about 0.1 mmolar, and C. determining said enzyme-labeled antigen in bound or unbound form.
Claim 23
Independent Claim
122 words
A method for the determination of an antigen in a biological fluid, said method comprising the steps of: A. in the presence of an antibody for said antigen, contacting a sample of said fluid with an analytical composition comprising vesicles, said vesicles comprising lipid membranes prepared from a phospholipid represented by the structure: ##STR6## wherein R' and R" are independently alkylene groups, and X is an alkylene quaternary ammonium cation, said vesicles having said antigen labeled with an enzyme encapsulated therein, and an outer surface essentially free of said antigen and said antibody, B. lysing said vesicles with a surfactant having a critical micelle concentration of at least about 0.1 mmolar, and C. determining said enzyme-labeled antigen in bound or unbound form.
Claim 24
Dependent Claim
76 words
A diagnostic test kit for the determination of an immunologically active ligand, said kit comprising (i) a receptor for said ligand, (ii) an analytical composition comprising vesicles, said vesicles comprising lipid membranes with a labeled analog of said ligand encapsulated therein, and having an outer surface which is essentially free of said ligand and said receptor, and (iii) a surfactant capable of lysing said vesicles having a critical micelle concentration of at least about 0.1 mmolar.
Claim 25
Dependent Claim
76 words
A diagnostic test kit for the determination of an immunologically active ligand, said kit comprising (i) a receptor for said ligand, (ii) an analytical composition comprising vesicles, said vesicles comprising lipid membranes with a labeled analog of said ligand encapsulated therein, and having an outer surface which is essentially free of said ligand and said receptor, and (iii) a surfactant capable of lysing said vesicles having a critical micelle concentration of at least about 0.1 mmolar.
References Cited (Backward Citations)
US3887698A
Cited
US3850578A
Cited
US3896217A
Cited
US3993754A
Cited
US4084967A
Cited
US4235792A
Cited
US4235871A
Cited
US4193983A
Cited
US4255411A
Cited
US4342739A
Cited
EP0014530A1
Cited
US4310505A
Cited
JPS5679255A
Cited
US4372745A
Cited
US4342826A
Cited
US4343895A
Cited
JPS5719661A
Cited
GB2079937A
Cited
GB2079936A
Cited
US4314021A
Cited
US4356256A
Cited
WO1983001571A1
Cited
US4429008A
Cited
US4429008B1
Cited
EP0090735A2
Cited
US4517303A
Cited
US4666830A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-06-21 | 1991-12-21 | 1992-06-21 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-06-21 | 1995-12-21 | 1996-06-21 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-06-21 | 1999-12-21 | 2000-06-21 | $7700.00 | Window Closed / Lapsed |