Patent US4757014A successfully refreshed from Google Patents.
US4757014A
Expired / Lapsed
US
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Immobilization of biologically active protein on a polymeric fibrous support
Assignee: 3M Co
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Filing: 1985-11-08
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Granted: 1988-07-12
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Expires: 2005-11-08
Abstract
Abstract A composite article is prepared comprising in sequence a fibrous polymeric support which has been subjected to a surface treatment to provide binding sites thereon, a layer of a protein immobilizer compound, and a biologically active protein. The surface treatment comprises coating the support with a 2 to 500 nm thick layer of inorganic oxide or subjecting the support to a plasma treatment. The protein immobilizer can be a polymer or silane-functional compound. The biologically active protein can be the enzyme, catalase, which has use in decomposing hydrogen peroxide when disinfecting contact lenses.
Inventors
- 👤 Carol E. Hendrickson
- 👤 Rosa Uy
- 👤 Arlene J. Mencke
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 15:53
CPC Classifications
A; A61; A61L; A61L12/00; A61L12/08; A61L12/12; A61L12/124; A61L12/126; A61L2/00; A61L2/16; A61L2/18; C; C11; C11D; C11D3/00; C11D3/0005; C11D3/0078; Y; Y10; Y10S; Y10S530/00; Y10S530/81; Y10S530/811; Y10S530/812; Y10S530/815
Claims Analysis
Total Claims: 34
Claim 1
Independent Claim
86 words
A composite article comprising in sequence: (a) a polymeric fibrous support having a large surface area and having on its surface a layer of inorganic oxide having a uniform thickness when dried in the range of 2 to 500 nm to provide binding sites thereon, (b) a layer of a protein immobilizer compound comprising a polymer or a silane-functional compound adhering to said layer of inorganic oxide and capable of immobilizing proteins, and (c) a biologically active protein bound to said layer of protein immobilizer compound.
Claim 2
Dependent Claim
86 words
A composite article comprising in sequence: (a) a polymeric fibrous support having a large surface area and having on its surface a layer of inorganic oxide having a uniform thickness when dried in the range of 2 to 500 nm to provide binding sites thereon, (b) a layer of a protein immobilizer compound comprising a polymer or a silane-functional compound adhering to said layer of inorganic oxide and capable of immobilizing proteins, and (c) a biologically active protein bound to said layer of protein immobilizer compound.
Claim 3
Dependent Claim
13 words
The article according to claim 1 wherein said support is woven or nonwoven.
Claim 4
Dependent Claim
11 words
The article according to claim 2 wherein said support is nonwoven.
Claim 5
Dependent Claim
26 words
The article according to claim 2 wherein said support is polyalkyhlene, polyvinyl chloride, polyamide, polyvinyl alcohol, polystyrene, polyarylsulfone, polyester, polycarbonate, polyacrylate, cellulosic, polyurethane, or combinations thereof.
Claim 6
Dependent Claim
15 words
The article according to claim 1 wherein said inorganic oxide layer comprises glass or clay.
Claim 7
Dependent Claim
15 words
The article according to claim 1 wherein said inorganic oxide layer is a gelled network.
Claim 8
Dependent Claim
14 words
The article according to claim 6 wherein said gelled network is a ceramic-precursor gel.
Claim 9
Dependent Claim
22 words
The article according to claim 1 wherein said polymer is a beta-hydroxyalkyleneamine-containing polymer, and the silane-functional compound is a silane-treated polycarbodiimide polymer.
Claim 10
Dependent Claim
21 words
The article according to claim 1 wherein said polymer is an amine adduct of epoxidized poly=cis-1,4-butadiene, epoxidized styrene/cis-1,4-butadiene, or polyglycidyl methacrylate.
Claim 11
Dependent Claim
16 words
The article according to claim 13 wherein said amine is dimethylamine, diethylamine, morpholine, piperidine, or n-propylamine.
Claim 12
Dependent Claim
12 words
The article according to claim 1 wherein said protein is an enzyme.
Claim 13
Dependent Claim
25 words
The article according to claim 11 wherein said enzyme is urease, glucose oxidase, invertase, peroxidase, catalase, papain, lipase, cellulase, dextranase, amylase, ribonuclease, carboxypeptidase or urokinase.
Claim 14
Dependent Claim
11 words
The article according to claim 11 wherein said enzyme is catalase.
Claim 15
Dependent Claim
12 words
The article according to claim 1 wherein said protein is an immunochemical.
Claim 16
Dependent Claim
13 words
The article according to claim 1 wherein said protein is an immunologically-active protein.
Claim 17
Dependent Claim
113 words
A composite article comprising in sequence: (a) a polymeric fibrous support having a large surface area, which surface has been subjected to a surface plasma treatment carried out at a frequency of 10 to 125 kilohertz with a power density in the range of 0.05 to 2.25 w/cm 2 gnerated between two electdodes in a gas at a pressure in the range of 10 mtorr to 10 torr to provide binding sites thereon, (b) a layer of a protein immobilizer compound comprising a polymer or a silane-functional compound adhering to the resulting treated surface and capable of immobilizing proteins, and (c) a biologically active protein bound to said layer of protein immobilizer compound.
Claim 18
Dependent Claim
113 words
A composite article comprising in sequence: (a) a polymeric fibrous support having a large surface area, which surface has been subjected to a surface plasma treatment carried out at a frequency of 10 to 125 kilohertz with a power density in the range of 0.05 to 2.25 w/cm 2 gnerated between two electdodes in a gas at a pressure in the range of 10 mtorr to 10 torr to provide binding sites thereon, (b) a layer of a protein immobilizer compound comprising a polymer or a silane-functional compound adhering to the resulting treated surface and capable of immobilizing proteins, and (c) a biologically active protein bound to said layer of protein immobilizer compound.
Claim 19
Dependent Claim
30 words
The article according to claim 16 wherein said plasma treatment utilizes a gas selected from the group consisting of air, oxygen, carbo dioxide, argon, helium, nitrous oxide, or water vapor.
Claim 20
Dependent Claim
14 words
The article according to claim 17, wherein said gas is air or carbon dioxide.
Claim 21
Dependent Claim
90 words
A composite article comprising in sequence; (a) a polymeric fibrous support having a large surface area and having on its surface a layer of inorganic oxide selected from the group consisting of glass and clay having a thickness when dried in the range of 2 to 500 nm to provide binding sites thereon, and (b) a layer of a protein immobilizer compound comprising a polymer or a silane-functional compound adhering to said layer of inorganic oxide, and (c) a biologically active protein bound to said layer or protein immobilizer compound.
Claim 22
Dependent Claim
90 words
A composite article comprising in sequence; (a) a polymeric fibrous support having a large surface area and having on its surface a layer of inorganic oxide selected from the group consisting of glass and clay having a thickness when dried in the range of 2 to 500 nm to provide binding sites thereon, and (b) a layer of a protein immobilizer compound comprising a polymer or a silane-functional compound adhering to said layer of inorganic oxide, and (c) a biologically active protein bound to said layer or protein immobilizer compound.
Claim 23
Dependent Claim
14 words
The article according to claim 19 wherein said layer of inorganic oxide is glass.
Claim 24
Dependent Claim
15 words
The article according to claim 19, wherein said inorganic oxide layer is a gelled network.
Claim 25
Dependent Claim
84 words
A composite article comprising in sequence; (a) a nonwoven polymeric fibrous support having a large surface area, (b) a layer on said fibrous support comprising a gelled network of silica particles to provide binding sites for a protein immobilizer compound, said layer having a uniform thickness when dried in the range of 2 to 500 nm, (c) a layer of a beta-hydroxyalkyleneamine-containing polymer bound to said layer of (b), and (d) a layer of biologically active catalase bound to said polymer layer of (c).
Claim 26
Dependent Claim
84 words
A composite article comprising in sequence; (a) a nonwoven polymeric fibrous support having a large surface area, (b) a layer on said fibrous support comprising a gelled network of silica particles to provide binding sites for a protein immobilizer compound, said layer having a uniform thickness when dried in the range of 2 to 500 nm, (c) a layer of a beta-hydroxyalkyleneamine-containing polymer bound to said layer of (b), and (d) a layer of biologically active catalase bound to said polymer layer of (c).
Claim 27
Dependent Claim
87 words
A composite article comprising in sequence: (a) a polymeric fibrous support having a large surface area and having on its surface a layer of inorganic oxide comprising at least one ceramic precursor having a thickness when dried in the range of 2 to 500 nm to provide binding sites thereon, and (b) a layer of a protein immobilizer compound comprising a polymer or a silane-functional compound adhering to said layer of inorganic oxide, and (c) a biologically active protein bound to said layer of protein immobilizer compound.
Claim 28
Dependent Claim
87 words
A composite article comprising in sequence: (a) a polymeric fibrous support having a large surface area and having on its surface a layer of inorganic oxide comprising at least one ceramic precursor having a thickness when dried in the range of 2 to 500 nm to provide binding sites thereon, and (b) a layer of a protein immobilizer compound comprising a polymer or a silane-functional compound adhering to said layer of inorganic oxide, and (c) a biologically active protein bound to said layer of protein immobilizer compound.
Claim 29
Independent Claim
96 words
A method for providing a composite article comprising the steps: (a) coating a woven or nonwoven polymeric fibrous support having a large surface area so as to provide said surface with binding sites for a protein immobilizer comound, said coating comprising a layer of inorganic oxide having a uniform thickness when dried in the range of 2 to 500 nm, (b) coating said layer of inorganic oxide with a layer of a protein immobilizer compound the comprising a polymer or a silane-functional compound, and (c) attaching a biologically-active protein to said layer of protein immobilizer compound.
Claim 30
Independent Claim
96 words
A method for providing a composite article comprising the steps: (a) coating a woven or nonwoven polymeric fibrous support having a large surface area so as to provide said surface with binding sites for a protein immobilizer comound, said coating comprising a layer of inorganic oxide having a uniform thickness when dried in the range of 2 to 500 nm, (b) coating said layer of inorganic oxide with a layer of a protein immobilizer compound the comprising a polymer or a silane-functional compound, and (c) attaching a biologically-active protein to said layer of protein immobilizer compound.
Claim 31
Dependent Claim
15 words
The method according to claim 24 wherein said inorganic oxide layer is a ceramic precursor.
Claim 32
Dependent Claim
15 words
The method according to claim 25 wherein said inorganic oxide layer is glass or clay.
Claim 33
Independent Claim
120 words
A method for providing a composite article comprising the steps: (a) surface treating a woven or nonwoven polymeric fibrous support having a large surface area so as to provide said surface with binding sites for a protein immobilizer compound, said treatment comprising a surface plasma treatment carried out at a frequency of 10 to 125 kiloherz with a power density in the range of 0.05 to 2.25 w/cm 2 generated between two electrodes in a gas at a pressure in the range of 10 mtorr to 10 torr, (b) coating said treated surface with a layer of a protein immobilizer compound comprising a polymer or a silane-functional compound, and (c) attaching a biologically-active protein to said layer of immobilizer compound.
Claim 34
Independent Claim
120 words
A method for providing a composite article comprising the steps: (a) surface treating a woven or nonwoven polymeric fibrous support having a large surface area so as to provide said surface with binding sites for a protein immobilizer compound, said treatment comprising a surface plasma treatment carried out at a frequency of 10 to 125 kiloherz with a power density in the range of 0.05 to 2.25 w/cm 2 generated between two electrodes in a gas at a pressure in the range of 10 mtorr to 10 torr, (b) coating said treated surface with a layer of a protein immobilizer compound comprising a polymer or a silane-functional compound, and (c) attaching a biologically-active protein to said layer of immobilizer compound.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1991-07-12 | 1992-01-11 | 1992-07-12 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-07-12 | 1996-01-11 | 1996-07-12 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-07-12 | 2000-01-11 | 2000-07-12 | $7700.00 | Window Closed / Lapsed |