Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
270 words
A proteinaceous substance having the following biochemical characteristics: (A) said substance has a high tumor specificity as evidenced by its strong cytolytic activity against mouse tumor cells L-929 and L-1210, and human carcinoma HeLa cells, and lack of cytotoxic activity against normal human fetus fibroblasts, normal human adult skin fibroblasts or normal Chinese hamster fibroblasts (Don, V-79); (B) said substance has a strong anti-tumor activity against Meth-A sarcoma, as evidenced by the fact that when said substance is administered intravenously to syngeneic BALB/c mice inoculated with Meth-A sarcoma at a quantity of more than 72 units/head, hemorrhagic necrosis of the tumor is observed, and subsequent successful implantation of Meth-A sarcoma cells in the same mice is not observed because of acquired immunity; (C) said substance has no pyrogenic effect on rabbits after intravenous injection; (D) said substance has a molecular weight of 45,000 plus or minus 5,000 as determined by gel filtration with Sepharcryl S-200; (E) said substance has an isoelectric point of 4.8 plus or minus 0.3; (F) said substance is sensitive to tyrpsin, but stable to heating at 56° C. for 30 minutes; (G) said substance is not glycosylated; and (H) said substance contains the following first 15 N-terminal amino acids: ##STR2## (I) said substance contains the following amino acid content: TABLE
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Amino Number of Amino Number of
Amino Number of
Acid Residues Acid Residues
Acid Residues
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Asn 15 Ala 12 Phe 6
Thr 6 Val 16 Lys 9
Ser 10 Met 1 His 3
Gln 20 Ile 5 Arg 3
Pro 9 Leu 21 Cys 2
Gly 10 Tyr 8 Trp 2
Total 156
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Claim 2
Dependent Claim
270 words
A proteinaceous substance having the following biochemical characteristics: (A) said substance has a high tumor specificity as evidenced by its strong cytolytic activity against mouse tumor cells L-929 and L-1210, and human carcinoma HeLa cells, and lack of cytotoxic activity against normal human fetus fibroblasts, normal human adult skin fibroblasts or normal Chinese hamster fibroblasts (Don, V-79); (B) said substance has a strong anti-tumor activity against Meth-A sarcoma, as evidenced by the fact that when said substance is administered intravenously to syngeneic BALB/c mice inoculated with Meth-A sarcoma at a quantity of more than 72 units/head, hemorrhagic necrosis of the tumor is observed, and subsequent successful implantation of Meth-A sarcoma cells in the same mice is not observed because of acquired immunity; (C) said substance has no pyrogenic effect on rabbits after intravenous injection; (D) said substance has a molecular weight of 45,000 plus or minus 5,000 as determined by gel filtration with Sepharcryl S-200; (E) said substance has an isoelectric point of 4.8 plus or minus 0.3; (F) said substance is sensitive to tyrpsin, but stable to heating at 56° C. for 30 minutes; (G) said substance is not glycosylated; and (H) said substance contains the following first 15 N-terminal amino acids: ##STR2## (I) said substance contains the following amino acid content: TABLE
______________________________________
Amino Number of Amino Number of
Amino Number of
Acid Residues Acid Residues
Acid Residues
______________________________________
Asn 15 Ala 12 Phe 6
Thr 6 Val 16 Lys 9
Ser 10 Met 1 His 3
Gln 20 Ile 5 Arg 3
Pro 9 Leu 21 Cys 2
Gly 10 Tyr 8 Trp 2
Total 156
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Claim 3
Dependent Claim
62 words
The substance of claim 1, wherein said substance is obtained by allowing cultured mammalian tumor cells to proliferate in complete growth medium containing calf fetal serum, wherein said tumor cells are histocytes showing phagocytic activity, followed by culturing said cells in a serum-free medium in order to obtain a supernatant liquid of the culture containing said proteinaceous substance, and isolating said substance.
Claim 4
Dependent Claim
18 words
The proteinaceous substance of claim 2, which is derived from histocyte-type J-774.1 reticulosarcoma cultured cells having phagocytic activity.
Claim 5
Dependent Claim
52 words
The proteinaceous substance of claim 2, which is derived from cloned culture cells derived from human lymph nodes, wherein said cultured cells are selected from the group consisting of U-937, RS-3, MC-8-3, MKC-12, RPMI-8866, ARH-77, WA and KCCL cells, wherein said cells are histocyte-type cells having a round shape and phagocytic activity.
Claim 6
Dependent Claim
43 words
The proteinaceous substance of claim 2, which is derived from cloned cultured cells which are tumor cells of human myeloma selected from the group consisting of K-562, D-98 and OST cells, and wherein said cultured cells have a round shape and phagocytic activity.
Claim 7
Dependent Claim
35 words
The proteinaceous substance according to claim 2, which is derived from a human Burkitt lymphoma selected from the group consisting of EB-3, Raji, and P3HR-1, wherein said cultured cells are histocyte-type cells having phagocytic activity.
Claim 8
Dependent Claim
27 words
The proteinaceous substance according to claim 2, wherein said substance is derived from hybridoma cells, wherein said hybridoma cells are fused U-937 cells and human B cells.
Claim 9
Dependent Claim
14 words
The proteinaceous substance of claim 7, wherein said human B cells are BALL-1 cells.
Claim 10
Dependent Claim
32 words
The proteinaceous substance according to claim 2, which is derived from mouse lymphoma cells selected from the group consisting of RAW-264, IC-21, P388D1 and PU5-1.8 cells, wherein said cells have phagocytic activity.
Claim 11
Dependent Claim
31 words
The proteinaceous substance of claim 2, wherein said substance is produced by adding a trace of 2-mercaptoethanol or a trace of phorbol myristate acetate to a series of said cultured cells.
Claim 12
Dependent Claim
53 words
A method for obtaining a substantially purified proteinaceous substance according to claim 2, wherein after the supernatant liquid cultured in the serum-free medium is precipitated with ammonium sulfate and is added to a gel filtrating column with Sephacryl S-200, the portion not absorbed with blue Separose is collected and passed through DEAE-Sephadex A-50.