Patent US4801540A successfully refreshed from Google Patents.
US4801540A
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PG gene and its use in plants
Assignee: Monsanto Co
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Filing: 1987-01-02
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Granted: 1989-01-31
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Expires: 2007-01-02
Abstract
Abstract Polygalacturonase DNA sequence and its use in modulating polygalacturonase expression in plant cells. DNA constructions are provided. The transit peptide finds use with heterologous peptides.
Inventors
- 👤 William R. Hiatt
- 👤 Raymond E. Sheehy
- 👤 Christine K. Shewmaker
- 👤 Jean C. Kridl
- 👤 Vic Knauf
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 10:41
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/11; C12N15/113; C12N15/1137; C12N15/63; C12N15/79; C12N15/82; C12N15/8216; C12N15/8218; C12N9/00; C12N9/14; C12N9/24; C12N9/2402; C12Y; C12Y302/00; C12Y302/01; C12Y302/01015; C12N2310/00; C12N2310/10; C12N2310/11; C12N2310/111
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
30 words
A DNA sequence which is uninterrupted, encodes tomato polygalacturonase (PG) and is flanked by at least one non-wild type DNA sequence 5' to or 3' to said polygalacturonase encoding sequence.
Claim 2
Dependent Claim
30 words
A DNA sequence which is uninterrupted, encodes tomato polygalacturonase (PG) and is flanked by at least one non-wild type DNA sequence 5' to or 3' to said polygalacturonase encoding sequence.
Claim 3
Dependent Claim
14 words
A DNA sequence according to claim 1, wherein said sequence is a cDNA sequence.
Claim 4
Dependent Claim
19 words
A DNA sequence according to claim 2, wherein said cDNA sequence encodes the tomato fruit PG2A isoform of polygalacturonase.
Claim 5
Dependent Claim
13 words
A DNA sequence according to claim 1, joined to a prokaryotic replication system.
Claim 6
Dependent Claim
20 words
A DNA sequence according to claim 4, comprising a marker for selection of a eukaryotic cell comprising said DNA sequence.
Claim 7
Dependent Claim
6 words
A cDNA encoding for tomato polygalacturonase.
Claim 8
Dependent Claim
6 words
A cDNA encoding for tomato polygalacturonase.
Claim 9
Dependent Claim
14 words
A cDNA according to claim 6, wherein said polygalacturonase is tomato fruit PG2A isoform.
Claim 10
Dependent Claim
41 words
A DNA construct comprising a DNA sequence of at least 15 base pairs of a DNA sequence encoding tomato polygalacturonase (PG) joined, in the opposite orientation for expression, 5' to the 3' terminus of a transcriptional initiation region functional in plants.
Claim 11
Dependent Claim
41 words
A DNA construct comprising a DNA sequence of at least 15 base pairs of a DNA sequence encoding tomato polygalacturonase (PG) joined, in the opposite orientation for expression, 5' to the 3' terminus of a transcriptional initiation region functional in plants.
Claim 12
Dependent Claim
17 words
A DNA construct according to claim 8, wherein said tomato polygalacturonase is the tomato fruit PG2A isoform.
Claim 13
Dependent Claim
25 words
A DNA construct according to claim 8, joined to a sequence encoding a marker capable of selection in a plant cell comprising said DNA construct.
Claim 14
Dependent Claim
32 words
A DNA construct according to claim 8, wherein said DNA sequence comprises a strand complementary to at least 200 and not greater than about 1420 nt of the messenger RNA of polygalacturonase.
Claim 15
Dependent Claim
43 words
A DNA construct comprising a transcriptional initiation region functional in plants joined at its 3' terminus to the 5' terminus of a tomato polygalacturonase transit peptide encoding sequence, said transit peptide encoding sequence joined to other than a sequence encoding mature tomato polygalacturonase.
Claim 16
Dependent Claim
43 words
A DNA construct comprising a transcriptional initiation region functional in plants joined at its 3' terminus to the 5' terminus of a tomato polygalacturonase transit peptide encoding sequence, said transit peptide encoding sequence joined to other than a sequence encoding mature tomato polygalacturonase.
Claim 17
Dependent Claim
60 words
A DNA construct comprising a transcription initiation region functional in a plant joined directly or through an intervening sequence to the 5' terminus of at least one of (a) at least 15 and no more than 259 nt of the 5'-terminal coding region of tomato polygalacturonase and (b) at least 39 nt of the 3'-terminal coding region of tomato polygalacturonase.
Claim 18
Dependent Claim
60 words
A DNA construct comprising a transcription initiation region functional in a plant joined directly or through an intervening sequence to the 5' terminus of at least one of (a) at least 15 and no more than 259 nt of the 5'-terminal coding region of tomato polygalacturonase and (b) at least 39 nt of the 3'-terminal coding region of tomato polygalacturonase.
Claim 19
Dependent Claim
16 words
A tomato plant cell comprising a DNA construct according to any of claims 8 to 13.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-01-31 | 1992-08-01 | 1993-01-31 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-01-31 | 1996-08-01 | 1997-01-31 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-01-31 | 2000-08-01 | 2001-01-31 | $7700.00 | Window Closed / Lapsed |