Patent US4812412A successfully refreshed from Google Patents.
US4812412A
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US
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Standard specimen and method of making and using same
Assignee: TOWER BUILDING EMPIRE STATE PLAZA ALBANY 12237
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Filing: 1987-02-26
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Granted: 1989-03-14
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Expires: 2007-02-26
Abstract
Abstract This invention provides a standard specimen useful for the quantitative evaluation of performance of stains, staining solutions and staining processes used on biological specimens, and also for the evaluation of dyestuffs. The standard specimens are comprised of extracts of biological materials containing protein and/or nucleic acid which are incorporated into a reproducible plastic matrix of controlled thickness. The standard specimen so produced is useful in the quantitative performance evaluation of a dyeing or staining process, thereby providing information for process control and quantitative analysis and comparisons. The invention also provides for the method of preparing the standard specimen of the invention, as well as for the method of using the standard specimen.
Inventors
- 👤 James N. Turner
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 07:41
CPC Classifications
G; G01; G01N; G01N1/00; G01N1/28; G01N1/30; G01N2001/2893; Y; Y10; Y10T; Y10T436/00; Y10T436/10; Y10T436/105831
Claims Analysis
Total Claims: 26
Claim 1
Independent Claim
119 words
A standard specimen for the quantitative evaluation of stain performance in staining processes which utilize dyes to stain cells or tissues which stained cells or tissue exhibit characteristic spectra when subjected to light transmission analysis, which standard specimen comprises a uniform amount of biological material extracted from cells or tissue incorporated into a plastic matrix which specimen-containing matrix is capable of being reproduced and which specimen-containing matrix is sectioned to a controlled thickness and stained and mounted and which specimen-containing matrix presents a uniform field constant from one area of the specimen-containing matrix to the next, and which exhibits spectra substantially the same as the characteristic spectra of the cells or tissue stained when subjected to light transmission analysis.
Claim 2
Dependent Claim
119 words
A standard specimen for the quantitative evaluation of stain performance in staining processes which utilize dyes to stain cells or tissues which stained cells or tissue exhibit characteristic spectra when subjected to light transmission analysis, which standard specimen comprises a uniform amount of biological material extracted from cells or tissue incorporated into a plastic matrix which specimen-containing matrix is capable of being reproduced and which specimen-containing matrix is sectioned to a controlled thickness and stained and mounted and which specimen-containing matrix presents a uniform field constant from one area of the specimen-containing matrix to the next, and which exhibits spectra substantially the same as the characteristic spectra of the cells or tissue stained when subjected to light transmission analysis.
Claim 3
Dependent Claim
13 words
A standard specimen according to claim 1 wherein the biological material contains protein.
Claim 4
Dependent Claim
14 words
A standard specimen according to claim 1 wherein the biological material contains nucleic acid.
Claim 5
Dependent Claim
27 words
A standard specimen according to claim 1 wherein the biological material is selected from the group consisting of bovine liver acetone powder, nucleoprotamine or defatted horse muscle.
Claim 6
Dependent Claim
14 words
A standard specimen according to claim 1, wherein the plastic matrix is an epoxy.
Claim 7
Dependent Claim
14 words
A standard specimen according to claim 5, wherein the epoxy is glycol methacrylate epoxy.
Claim 8
Dependent Claim
96 words
A process for the preparation of a standard specimen for the quantitative evaluation of stain performance in staining processes which utilize dyes to stain cells or tissue and, which comprises incorporating a uniform amount of biological material extracted from cells or tissue into a plastic matrix, which specimen-containing matrix is capable of being reproduced and which specimen-containing matrix is sectioned to a controlled thickness and stained and mounted on a slide and which specimen-containing matrix presents a uniform field constant from one area of the specimen-containing matrix to the next when subjected to light transmission analysis.
Claim 9
Dependent Claim
96 words
A process for the preparation of a standard specimen for the quantitative evaluation of stain performance in staining processes which utilize dyes to stain cells or tissue and, which comprises incorporating a uniform amount of biological material extracted from cells or tissue into a plastic matrix, which specimen-containing matrix is capable of being reproduced and which specimen-containing matrix is sectioned to a controlled thickness and stained and mounted on a slide and which specimen-containing matrix presents a uniform field constant from one area of the specimen-containing matrix to the next when subjected to light transmission analysis.
Claim 10
Dependent Claim
13 words
A process according to in claim 7, wherein the biological material contains protein.
Claim 11
Dependent Claim
13 words
A process according to claim 7, wherein the biological material contains nucleic acid.
Claim 12
Dependent Claim
26 words
A process according to claim 7, wherein the biological material is selected from the group consisting of bovine liver acetone powder, nucleoprotamine or defatted horse muscle.
Claim 13
Dependent Claim
13 words
A process according to claim 7, wherein the plastic matrix is an epoxy.
Claim 14
Dependent Claim
14 words
A process according to in claim 11, wherein the epoxy is glycol methacrylate epoxy.
Claim 15
Independent Claim
117 words
A method for evaluating stain or dye performance in staining processes which utilize dyes to stain cells or tissue, which comprises comparing, by means of quantitative analysis of parameters dependent on light transmission, a specimen treated with the stain or dye with a standard specimen, which standard specimen comprises a uniform amount of biological material extracted from cells or tissue which is incorporated into a plastic matrix, which specimen-containing matrix is capable of being reproduced and which specimen-containing matrix is sectioned to a controlled thickness ad stained and placed on a slide and which specimen-containing matrix presents a uniform field constant from one area of the specimen-containing matrix to the next when subjected to light transmission analysis.
Claim 16
Independent Claim
117 words
A method for evaluating stain or dye performance in staining processes which utilize dyes to stain cells or tissue, which comprises comparing, by means of quantitative analysis of parameters dependent on light transmission, a specimen treated with the stain or dye with a standard specimen, which standard specimen comprises a uniform amount of biological material extracted from cells or tissue which is incorporated into a plastic matrix, which specimen-containing matrix is capable of being reproduced and which specimen-containing matrix is sectioned to a controlled thickness ad stained and placed on a slide and which specimen-containing matrix presents a uniform field constant from one area of the specimen-containing matrix to the next when subjected to light transmission analysis.
Claim 17
Dependent Claim
37 words
A method for evaluating stain or dye performance according to claim 13, wherein the quantitative analysis is light transmission analysis which compares the respective light transmission of the specimen being evaluated with that of the standard specimen.
Claim 18
Independent Claim
105 words
A method for evaluating a biological specimen comprising cells or tissue which method comprises comparing by means of quantitative analysis of parameters dependent on light transmission, the specimen to be evaluated with a standard specimen, which standard specimen comprises a uniform amount of biological material extracted from cells or tissue incorporated into a plastic matrix, which specimen-containing matrix is capable of being reproduced and which specimen-containing matrix is sectioned to a controlled thickness and stained and placed on a slide, and which specimen-containing matrix presents a uniform field constant from one area of the specimen-containing matrix to the next when subjected to light transmission analysis.
Claim 19
Independent Claim
105 words
A method for evaluating a biological specimen comprising cells or tissue which method comprises comparing by means of quantitative analysis of parameters dependent on light transmission, the specimen to be evaluated with a standard specimen, which standard specimen comprises a uniform amount of biological material extracted from cells or tissue incorporated into a plastic matrix, which specimen-containing matrix is capable of being reproduced and which specimen-containing matrix is sectioned to a controlled thickness and stained and placed on a slide, and which specimen-containing matrix presents a uniform field constant from one area of the specimen-containing matrix to the next when subjected to light transmission analysis.
Claim 20
Dependent Claim
18 words
A method for evaluating a biological specimen according to claim 15, wherein the quantitative analysis is ploidy analysis.
Claim 21
Dependent Claim
20 words
A method for evaluating a biological specimen according to claim 15, wherein the quantitative analysis is integrated optical density analysis.
Claim 22
Dependent Claim
19 words
A method for evaluating a biological specimen according to claim 15, wherein the quantitative analysis is light scattering analysis.
Claim 23
Dependent Claim
22 words
A method for evaluating a biological specimen according to claim 15, wherein the quantitative analysis is the analysis of light polarization effects.
Claim 24
Dependent Claim
18 words
A method for evaluating a biological specimen according to claim 15, wherein the quantitative analysis is fluorescence analysis.
Claim 25
Independent Claim
136 words
A method for evaluating stain or dye performance in staining processes which utilize dyes to stain cells or tissues, which comprises comparing a specimen treated with the stain or dye with a standard specimen, which comparison is carried out by means of quantitative analysis comparing the respective spectra when subjected to light transmission analysis of the specimen to be evaluated with the standard specimen, and which standard specimen comprises a uniform amount of nucleoprotamine which is incorporated into an epoxy matrix and mounted on a slide, which specimen-containing matrix is capable of being reproduced and which specimen-containing matrix is sectioned to a controlled thickness and stained and placed on a slide, and which specimen-containing matrix presents a uniform field constant from one area of the specimen-containing matrix to the next when subjected to light transmission analysis.
Claim 26
Independent Claim
136 words
A method for evaluating stain or dye performance in staining processes which utilize dyes to stain cells or tissues, which comprises comparing a specimen treated with the stain or dye with a standard specimen, which comparison is carried out by means of quantitative analysis comparing the respective spectra when subjected to light transmission analysis of the specimen to be evaluated with the standard specimen, and which standard specimen comprises a uniform amount of nucleoprotamine which is incorporated into an epoxy matrix and mounted on a slide, which specimen-containing matrix is capable of being reproduced and which specimen-containing matrix is sectioned to a controlled thickness and stained and placed on a slide, and which specimen-containing matrix presents a uniform field constant from one area of the specimen-containing matrix to the next when subjected to light transmission analysis.
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