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US4835708A
Expired / Lapsed
US
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Method and apparatus for analysis of chromatograms using cross-correlation and moment analysis
Assignee: Newport Corp USA
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Filing: 1987-07-06
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Granted: 1989-05-30
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Expires: 2007-07-06
Abstract
Abstract A method and apparatus for comparing substances against other substances or against themselves over time. The method involves taking a chromatogram from each substance and converting each chromatogram to a histogram. The histograms have one bar for each peak on the chromatogram. These histograms are then cross-correlated and the 0th, 1st, 2nd and 3rd moments of the cross-correlation function are calculated. A similarity number in terms of the number of peaks of difference between the two samples compared is then generated by dividing the 3rd moment by the 2nd moment, and the results are displayed. The apparatus of the invention provides the means to gather the data needed for the chromatograms, calculate the histograms, cross-correlation functions and moments and the similarity number and display the result.
Inventors
- 👤 Stephen Frans
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 13:00
CPC Classifications
G; G01; G01N; G01N30/00; G01N30/02; G01N30/86; G01N30/8675; G01N30/8679
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
82 words
An apparatus for comparing a degree of similarity of two substances comprising: means for injecting the two substances into a chromatography column; means for converting concentrations of various components in effluent streams of the two substances into chromatograms and then into two histograms, one for each substance; means for performing a cross-correlation on the two histograms and calculating 0th, 1st, 2nd and 3rd moments of a cross-correlation function; and means for displaying a ratio of the 3rd moment to the 2nd moment.
Claim 2
Independent Claim
82 words
An apparatus for comparing a degree of similarity of two substances comprising: means for injecting the two substances into a chromatography column; means for converting concentrations of various components in effluent streams of the two substances into chromatograms and then into two histograms, one for each substance; means for performing a cross-correlation on the two histograms and calculating 0th, 1st, 2nd and 3rd moments of a cross-correlation function; and means for displaying a ratio of the 3rd moment to the 2nd moment.
Claim 3
Independent Claim
125 words
An apparatus for comparing a degree of similarity of an unknown substance to a group of substances comprising: (1) means for injecting the unknown substance into a chromatography column and converting concentrations of various components in an effluent stream of the substance into a chromatogram and then into a histogram; (2) means for performing a cross-correlation on the histogram of the injected substance and the histogram, previously prepared, for one of the substances in the group of substances and calculating 0th, 1st, 2nd and 3rd moments of a cross-correlation function; (3) means for displaying a ratio of the 3rd moment to the 2nd moment calculated in step 2; and (4) means for repeating steps 2 and 3 for each substance in the group of substances.
Claim 4
Independent Claim
125 words
An apparatus for comparing a degree of similarity of an unknown substance to a group of substances comprising: (1) means for injecting the unknown substance into a chromatography column and converting concentrations of various components in an effluent stream of the substance into a chromatogram and then into a histogram; (2) means for performing a cross-correlation on the histogram of the injected substance and the histogram, previously prepared, for one of the substances in the group of substances and calculating 0th, 1st, 2nd and 3rd moments of a cross-correlation function; (3) means for displaying a ratio of the 3rd moment to the 2nd moment calculated in step 2; and (4) means for repeating steps 2 and 3 for each substance in the group of substances.
Claim 5
Independent Claim
98 words
An apparatus for comparing chromatograms comprising: means for detecting components in an effluent stream of a chromatography column for two samples injected separately into said chromatography column and generating a signal indicative of a concentration of each component in the effluent stream as each sample is injected into said column; means for converting said signals to two chromatograms; means for performing a cross-correlation on the two chromatograms; means for calculating 0th, 1st, 2nd and 3rd moments of a cross-correlation function; and means for calculating a ratio of said 3rd moment to said 2nd moment and displaying said ratio.
Claim 6
Independent Claim
98 words
An apparatus for comparing chromatograms comprising: means for detecting components in an effluent stream of a chromatography column for two samples injected separately into said chromatography column and generating a signal indicative of a concentration of each component in the effluent stream as each sample is injected into said column; means for converting said signals to two chromatograms; means for performing a cross-correlation on the two chromatograms; means for calculating 0th, 1st, 2nd and 3rd moments of a cross-correlation function; and means for calculating a ratio of said 3rd moment to said 2nd moment and displaying said ratio.
Claim 7
Dependent Claim
64 words
The apparatus of claim 3 wherein said means for converting the signals into chromatograms includes means for converting the chromatograms into histograms with one bar for each peak in the chromatogram, with each bar having a height representing an area under the corresponding peak, and wherein the means for performing cross-correlation performs the cross-correlation on the chromatograms after they have been converted into histograms.
Claim 8
Independent Claim
94 words
A method of comparing two chemical substances comprising the steps of: (1) separately injecting a sample of each of the substances to be compared into a chromatography column; (2) detecting for each sample components in an effluent stream and converting concentration and time of appearance data so gathered into a chromatogram for each sample, thus producing two chromatograms; (3) performing a cross-correlation between the two chromatograms; (4) calculating 0th, 1st, 2nd and 3rd moments of a cross-correlation function; (5) calculating a ratio of the 3rd moment to the 2nd moment and displaying the ratio.
Claim 9
Independent Claim
94 words
A method of comparing two chemical substances comprising the steps of: (1) separately injecting a sample of each of the substances to be compared into a chromatography column; (2) detecting for each sample components in an effluent stream and converting concentration and time of appearance data so gathered into a chromatogram for each sample, thus producing two chromatograms; (3) performing a cross-correlation between the two chromatograms; (4) calculating 0th, 1st, 2nd and 3rd moments of a cross-correlation function; (5) calculating a ratio of the 3rd moment to the 2nd moment and displaying the ratio.
Claim 10
Dependent Claim
59 words
The method of claim 5 further comprising the step of converting each chromatogram to a histogram with one bar per peak, with each bar having a height representing an area under the peak prior to performing the cross-correlation function and wherein the step of calculating the cross-correlation function is performed after the chromatograms have been converted to the histograms.
Claim 11
Dependent Claim
24 words
The method of claim 6 where all the steps are repeated to compare one unknown chemical substance to a plurality of known chemical substances.
Claim 12
Dependent Claim
63 words
The method of claim 7 wherein the steps of generating the histograms for each cross-correlation calculation are modified so that the unknown sample is injected into the chromatography column only once and its histogram is compared by the cross-correlation and moment ratio calculation steps, one at a time, to each of a plurality of histograms, each representing a previously prepared and stored histogram.
Claim 13
Independent Claim
85 words
A method of comparing two substances for chemical similarity comprising the steps of: (1) reading digital data defining a chromatogram for each of the two substances into a digital computer; (2) converting the digital data for each chromatogram into a histogram; (3) calculating a cross-correlation function of the two histograms; (4) calculating 0th, 1st, 2nd and 3rd moments of the cross-correlation function; (5) calculating and displaying a ratio of the 3rd moment to the 2nd moment; and (6) displaying identification data for the two substances.
Claim 14
Independent Claim
85 words
A method of comparing two substances for chemical similarity comprising the steps of: (1) reading digital data defining a chromatogram for each of the two substances into a digital computer; (2) converting the digital data for each chromatogram into a histogram; (3) calculating a cross-correlation function of the two histograms; (4) calculating 0th, 1st, 2nd and 3rd moments of the cross-correlation function; (5) calculating and displaying a ratio of the 3rd moment to the 2nd moment; and (6) displaying identification data for the two substances.
Claim 15
Dependent Claim
32 words
The method of claim 9, wherein steps 1 through 6 are repeated for a plurality of pairs of substances by utilizing stored digital data relating to chromatographs of the pairs of substances.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-05-29 | 1992-11-28 | 1993-05-30 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-05-29 | 1996-11-28 | 1997-05-30 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-05-29 | 2000-11-28 | 2001-05-30 | $7700.00 | Window Closed / Lapsed |