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US4889798A
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Heterologous system for the detection of chemically labeled DNA and other biological materials providing a receptor or target moiety thereon
Assignee: Enzo Diagnostics Inc
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Filing: 1987-02-13
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Granted: 1989-12-26
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Expires: 2007-02-13
Abstract
Abstract In accordance with the practices of this invention, there is provided a heterologous detection system and components useful in conjunction therewith and kits for carrying out the heterologous detection system. The heterologous detection system employs a heterologous entity and a signalling entity. The heterologous entity contains two free complex forming sites that form different complexes, i.e., two kinds of complexes can be formed. The first free complex forming site is utilized to recognize the labeled probe and the second free complex forming site is utilized to recognize the signalling entity, with each complex formed being different. The signalling entity contains a free complex forming site that can recognize the second free complex forming site of the heterologous entity and a signalling portion that is capable of generating a signal. An example of a heterologous detection system that can be utilized to detect biotinylated probe is a system that contains a heterologous entity comprising avidin and a lectin and a signalling entity comprising a glycosylated enzyme. The avidin can recognize the biotin of the probe and the lectin can recognize the glycosyl group of the enzyme. The enzyme is capable of generating a signal when treated with a suitable reagent. Such a heterologous detection system provides both a quick and highly sensitive technique for detecting a biotinylated probe.
Inventors
- 👤 Elazar Rabbani
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 09:21
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6813; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; G01N33/532; G01N33/535; Y; Y10; Y10S; Y10S435/00; Y10S435/803; Y10S435/81; Y10S436/00; Y10S436/807; Y10S436/808; Y10S436/824; Y10S436/827
Claims Analysis
Total Claims: 40
Claim 1
Independent Claim
70 words
A composition for detecting the presence or absence of an analyte in a sample, which composition comprises: a biotinylated polynucleotide specifically bindable with said analyte; a heterologous entity comprising streptavidin and a lectin; and a signaling entity which comprises a third free complex forming site which is specifically bindable with said lectin and, attached thereto, a signal generating portion capable of forming a signal associated with detection of said analyte.
Claim 2
Dependent Claim
70 words
A composition for detecting the presence or absence of an analyte in a sample, which composition comprises: a biotinylated polynucleotide specifically bindable with said analyte; a heterologous entity comprising streptavidin and a lectin; and a signaling entity which comprises a third free complex forming site which is specifically bindable with said lectin and, attached thereto, a signal generating portion capable of forming a signal associated with detection of said analyte.
Claim 3
Dependent Claim
70 words
A composition for detecting the presence or absence of an analyte in a sample, which composition comprises: a biotinylated polynucleotide specifically bindable with said analyte; a heterologous entity comprising avidin and a lectin; and a signaling entity which comprises a third free complex forming site which is specifically bindable with said lectin and, attached thereto, a signal generating portion capable of forming a signal associated with detection of said analyte.
Claim 4
Dependent Claim
70 words
A composition for detecting the presence or absence of an analyte in a sample, which composition comprises: a biotinylated polynucleotide specifically bindable with said analyte; a heterologous entity comprising avidin and a lectin; and a signaling entity which comprises a third free complex forming site which is specifically bindable with said lectin and, attached thereto, a signal generating portion capable of forming a signal associated with detection of said analyte.
Claim 5
Dependent Claim
81 words
A composition for detecting the presence or absence of an analyte in a sample, which composition comprises: a biotinylated polynucleotide specifically bindable with said analyte; a heterologous entity comprising a lectin that is complexed with a glycosylated enzyme that is biotinylated; or streptavidin; and a signaling entity which comprises a third free complex forming site which is specifically bindable with said glycosylated enzyme and, attached thereto, a signal generating portion capable of forming a signal associated with detection of said analyte.
Claim 6
Dependent Claim
81 words
A composition for detecting the presence or absence of an analyte in a sample, which composition comprises: a biotinylated polynucleotide specifically bindable with said analyte; a heterologous entity comprising a lectin that is complexed with a glycosylated enzyme that is biotinylated; or streptavidin; and a signaling entity which comprises a third free complex forming site which is specifically bindable with said glycosylated enzyme and, attached thereto, a signal generating portion capable of forming a signal associated with detection of said analyte.
Claim 7
Dependent Claim
35 words
The composition of claims 1, 2 or 3 wherein said lectin is selected from the group consisting of Concanavalin A, soybean lectin, wheatgerm lectin, lotus seed lectin, potato lectin, dilichos biflorus agglutinin and lentil lectin.
Claim 8
Dependent Claim
11 words
The composition of claim 4 wherein said lectin is Concanavalin A.
Claim 9
Dependent Claim
78 words
A composition for detecting the presence or absence of an analyte in a sample, which composition comprises: a biotinylated polynucleotide specifically bindable with said analyte: a heterologous entity comprising avidin or streptavidin complexed with a biotinylated enzyme that is glycosylated; and a signaling entity which comprises a third free complex forming site which is specifically bindable with said glycosylated enzyme and, attached thereto, a signal generating portion capable of forming a signal associated with detection of said analyte.
Claim 10
Dependent Claim
78 words
A composition for detecting the presence or absence of an analyte in a sample, which composition comprises: a biotinylated polynucleotide specifically bindable with said analyte: a heterologous entity comprising avidin or streptavidin complexed with a biotinylated enzyme that is glycosylated; and a signaling entity which comprises a third free complex forming site which is specifically bindable with said glycosylated enzyme and, attached thereto, a signal generating portion capable of forming a signal associated with detection of said analyte.
Claim 11
Dependent Claim
72 words
A composition for detecting the presence or absence of an analyte in a sample, which composition comprises: a biotinylated polynucleotide specifically bindable with said analyte; a heterologous entity comprising streptavidin and a glycosyl group; and a signaling entity which comprises a third free complex forming site which is specifically bindable with said glycosyl group and, attached thereto, a signal generating portion capable of forming a signal associated with detection of said analyte.
Claim 12
Dependent Claim
72 words
A composition for detecting the presence or absence of an analyte in a sample, which composition comprises: a biotinylated polynucleotide specifically bindable with said analyte; a heterologous entity comprising streptavidin and a glycosyl group; and a signaling entity which comprises a third free complex forming site which is specifically bindable with said glycosyl group and, attached thereto, a signal generating portion capable of forming a signal associated with detection of said analyte.
Claim 13
Dependent Claim
70 words
A composition for detecting the presence or absence of an analyte in a sample, which composition comprises: a glycosylated polynucleotide specifically bindable with said analyte; a heterologous entity comprising avidin and a lectin; and a signaling entity which comprises a third free complex forming site which is specifically bindable with said avidin and, attached thereto, a signal generating portion capable of forming a signal associated with detection of said analyte.
Claim 14
Dependent Claim
70 words
A composition for detecting the presence or absence of an analyte in a sample, which composition comprises: a glycosylated polynucleotide specifically bindable with said analyte; a heterologous entity comprising avidin and a lectin; and a signaling entity which comprises a third free complex forming site which is specifically bindable with said avidin and, attached thereto, a signal generating portion capable of forming a signal associated with detection of said analyte.
Claim 15
Dependent Claim
70 words
A composition for detecting the presence or absence of an analyte in a sample, which composition comprises: a glycosylated polynucleotide specifically bindable with said analyte; a heterologous entity comprising streptavidin and a lectin; and a signaling entity which comprises a third free complex forming site which is specifically bindable with said streptavidin and, attached thereto, a signal generating portion capable of forming a signal associated with detection of said analyte.
Claim 16
Dependent Claim
70 words
A composition for detecting the presence or absence of an analyte in a sample, which composition comprises: a glycosylated polynucleotide specifically bindable with said analyte; a heterologous entity comprising streptavidin and a lectin; and a signaling entity which comprises a third free complex forming site which is specifically bindable with said streptavidin and, attached thereto, a signal generating portion capable of forming a signal associated with detection of said analyte.
Claim 17
Dependent Claim
70 words
A composition for detecting the presence or absence of an analyte in a sample, which composition comprises: a glycosylated polynucleotide specifically bindable with said analyte; a heterologous entity comprising biotin and a lectin; and a signaling entity which comprises a third free complex forming site which is specifically bindable with said biotin and, attached thereto, a signal generating portion capable of forming a signal associated with detection of said analyte.
Claim 18
Dependent Claim
70 words
A composition for detecting the presence or absence of an analyte in a sample, which composition comprises: a glycosylated polynucleotide specifically bindable with said analyte; a heterologous entity comprising biotin and a lectin; and a signaling entity which comprises a third free complex forming site which is specifically bindable with said biotin and, attached thereto, a signal generating portion capable of forming a signal associated with detection of said analyte.
Claim 19
Dependent Claim
35 words
The composition of claims 8, 9, or 10 wherein said lectin is selected from the group consisting of Concanavalin A, soybean lectin, wheatgerm lectin, lotus seed lectin, potato lectin, dilicho biflorus agglutinin and lentil lectin.
Claim 20
Dependent Claim
11 words
The composition of claim 11 wherein said lectin is Concanavalin A.
Claim 21
Dependent Claim
94 words
A kit useful for detecting the presence or absence of an analyte in a sample, which kit comprises, in packaged combination, the following: (a) a first container having therein a labeled probe which comprises a biotinylated polynucleotide; (b) a second container having therein a heterologous entity which comprises streptavidin and a lectin; (c) a third container having therein a signalling entity which comprises a third free complex forming site which is specifically bindable with said lectin and, attached thereto, a signal generating portion capable of forming a signal associated with detection of said analyte.
Claim 22
Dependent Claim
94 words
A kit useful for detecting the presence or absence of an analyte in a sample, which kit comprises, in packaged combination, the following: (a) a first container having therein a labeled probe which comprises a biotinylated polynucleotide; (b) a second container having therein a heterologous entity which comprises streptavidin and a lectin; (c) a third container having therein a signalling entity which comprises a third free complex forming site which is specifically bindable with said lectin and, attached thereto, a signal generating portion capable of forming a signal associated with detection of said analyte.
Claim 23
Dependent Claim
94 words
A kit useful for detecting the presence or absence of an analyte in a sample, which kit comprises, in packaged combination, the following: (a) a first container having therein a labeled probe which comprises a biotinylated polynucleotide; (b) a second container having therein a heterologous entity which comprises avidin and a lectin; (c) a third container having therein a signalling entity which comprises a third free complex forming site which is specifically bindable with said lectin and, attached thereto, a signal generating portion capable of forming a signal associated with detection of said analyte.
Claim 24
Dependent Claim
94 words
A kit useful for detecting the presence or absence of an analyte in a sample, which kit comprises, in packaged combination, the following: (a) a first container having therein a labeled probe which comprises a biotinylated polynucleotide; (b) a second container having therein a heterologous entity which comprises avidin and a lectin; (c) a third container having therein a signalling entity which comprises a third free complex forming site which is specifically bindable with said lectin and, attached thereto, a signal generating portion capable of forming a signal associated with detection of said analyte.
Claim 25
Dependent Claim
101 words
A kit useful for detecting the presence or absence of an analyte in a sample, which kit comprises, in packaged combination, the following: (a) a first container having therein a labeled probe which comprises a biotinylated polynucleotide; (b) a second container having therein a heterologous entity which comprises a lectin that is complexed with a glycosylated enzyme that is biotinylated; a third container having therein a signalling entity which comprises a third free complex forming site which is specifically bindable with said lectin and, attached thereto, a signal generating portion capable of forming a signal associated with detection of said analyte.
Claim 26
Dependent Claim
101 words
A kit useful for detecting the presence or absence of an analyte in a sample, which kit comprises, in packaged combination, the following: (a) a first container having therein a labeled probe which comprises a biotinylated polynucleotide; (b) a second container having therein a heterologous entity which comprises a lectin that is complexed with a glycosylated enzyme that is biotinylated; a third container having therein a signalling entity which comprises a third free complex forming site which is specifically bindable with said lectin and, attached thereto, a signal generating portion capable of forming a signal associated with detection of said analyte.
Claim 27
Dependent Claim
35 words
The kit of claims 13, 14 or 15 wherein said lectin is selected from the group consisting of Concanavalin A, soybean lectin, wheatgerm lectin, lotus seed lectin, potato lectin, dilichos biflorus agglutinin and lentil lectin.
Claim 28
Dependent Claim
11 words
The kit of claim 16 wherein said lectin is Concanavalin A.
Claim 29
Dependent Claim
96 words
A kit useful for detecting the presence or absence of an analyte in a sample, which kit comprises, in packaged combination, the following: (a) a first container having therein a labeled probe which comprises a biotinylated polynucleotide; (b) a second container having therein a heterologous entity which comprises streptavidin and a glycosyl group; (c) a third container having therein a signalling entity which comprises a third free complex forming site which is specifically bindable with said glycosyl group and, attached thereto, a signal generating portion capable of forming a signal associated with detection of said analyte.
Claim 30
Dependent Claim
96 words
A kit useful for detecting the presence or absence of an analyte in a sample, which kit comprises, in packaged combination, the following: (a) a first container having therein a labeled probe which comprises a biotinylated polynucleotide; (b) a second container having therein a heterologous entity which comprises streptavidin and a glycosyl group; (c) a third container having therein a signalling entity which comprises a third free complex forming site which is specifically bindable with said glycosyl group and, attached thereto, a signal generating portion capable of forming a signal associated with detection of said analyte.
Claim 31
Dependent Claim
105 words
A kit useful for detecting the presence or absence of an analyte in a sample, which kit comprises, in packaged combination, the following: (a) a first container having therein a labeled probe which comprises a biotinylated polynucleotide; (b) a second container having therein a heterologous entity which comprises avidin or streptavidin complexed with a biotinylated enzyme that is glycosylated; (c) a third container having therein a signalling entity which comprises a third free complex forming site which is specifically bindable with said biotinylated enzyme that is glycosylated and, attached thereto, a signal generating portion capable of forming a signal associated with detection of said analyte.
Claim 32
Dependent Claim
105 words
A kit useful for detecting the presence or absence of an analyte in a sample, which kit comprises, in packaged combination, the following: (a) a first container having therein a labeled probe which comprises a biotinylated polynucleotide; (b) a second container having therein a heterologous entity which comprises avidin or streptavidin complexed with a biotinylated enzyme that is glycosylated; (c) a third container having therein a signalling entity which comprises a third free complex forming site which is specifically bindable with said biotinylated enzyme that is glycosylated and, attached thereto, a signal generating portion capable of forming a signal associated with detection of said analyte.
Claim 33
Dependent Claim
94 words
A kit useful for detecting the presence or absence of an analyte in a sample, which kit comprises, in packaged combination, the following: (a) a first container having therein a labeled probe which comprises a glycosylated polynucleotide; (b) a second container having therein a heterologous entity which comprises avidin and a lectin; (c) a third container having therein a signalling entity which comprises a third free complex forming site which is specifically bindable with said avidin and, attached thereto, a signal generating portion capable of forming a signal associated with detection of said analyte.
Claim 34
Dependent Claim
94 words
A kit useful for detecting the presence or absence of an analyte in a sample, which kit comprises, in packaged combination, the following: (a) a first container having therein a labeled probe which comprises a glycosylated polynucleotide; (b) a second container having therein a heterologous entity which comprises avidin and a lectin; (c) a third container having therein a signalling entity which comprises a third free complex forming site which is specifically bindable with said avidin and, attached thereto, a signal generating portion capable of forming a signal associated with detection of said analyte.
Claim 35
Dependent Claim
94 words
A kit useful for detecting the presence or absence of an analyte in a sample, which kit comprises, in packaged combination, the following: (a) a first container having therein a labeled probe which comprises a glycosylated polynucleotide; (b) a second container having therein a heterologous entity which comprises streptavidin and a lectin; (c) a third container having therein a signalling entity which comprises a third free complex forming site which is specifically bindable with said streptavidin and, attached thereto, a signal generating portion capable of forming a signal associated with detection of said analyte.
Claim 36
Dependent Claim
94 words
A kit useful for detecting the presence or absence of an analyte in a sample, which kit comprises, in packaged combination, the following: (a) a first container having therein a labeled probe which comprises a glycosylated polynucleotide; (b) a second container having therein a heterologous entity which comprises streptavidin and a lectin; (c) a third container having therein a signalling entity which comprises a third free complex forming site which is specifically bindable with said streptavidin and, attached thereto, a signal generating portion capable of forming a signal associated with detection of said analyte.
Claim 37
Dependent Claim
94 words
A kit useful for detecting the presence or absence of an analyte in a sample, which kit comprises, in packaged combination, the following: (a) a first container having therein a labeled probe which comprises a glycosylated polynucleotide; (b) a second container having therein a heterologous entity which comprises biotin and a lectin; (c) a third container having therein a signalling entity which comprises a third free complex forming site which is specifically bindable with said biotin and, attached thereto, a signal generating portion capable of forming a signal associated with detection of said analyte.
Claim 38
Dependent Claim
94 words
A kit useful for detecting the presence or absence of an analyte in a sample, which kit comprises, in packaged combination, the following: (a) a first container having therein a labeled probe which comprises a glycosylated polynucleotide; (b) a second container having therein a heterologous entity which comprises biotin and a lectin; (c) a third container having therein a signalling entity which comprises a third free complex forming site which is specifically bindable with said biotin and, attached thereto, a signal generating portion capable of forming a signal associated with detection of said analyte.
Claim 39
Dependent Claim
34 words
The kit of claims 20, 21 or 22 wherein said lectin is selected from the group consisting Concanavalin A, soybean lectin, wheatgerm lectin, lotus seed lectin, potato lectin, dilichos bilforus agglutinin and lentil lectin.
Claim 40
Dependent Claim
11 words
The kit of claim 23 wherein said lectin is Concanavalin A.
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USPTO Annuity & Maintenance Windows
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| 3.5 Years (Year 4) | 1992-12-25 | 1993-06-26 | 1993-12-26 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-12-25 | 1997-06-26 | 1997-12-26 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-12-25 | 2001-06-26 | 2001-12-26 | $7700.00 | Window Closed / Lapsed |