Patent US4954444A successfully refreshed from Google Patents.
US4954444A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Enzyme immobilization and bioaffinity separations with perfluorocarbon polymer-based supports
Assignee: Fleet Capital Corp
•
Filing: 1987-12-17
•
Granted: 1990-09-04
•
Expires: 2007-12-17
Abstract
Abstract A bioaffinity separation method is provided along with a solid affinity support utilized in that method. Additionally, immobilized enzyme systems are provided for use as enzyme electrode systems. The support is based on an inert perfluorocarbon polymer carrier with ligands or binders attached to its surface through a highly fluorinated isocyanate anchor group. Methods for preparing such supports and their use in capturing target molecules from samples and in analytical applications are also provided.
Inventors
- 👤 John William d. Eveleigh
- 👤 Robert K. Kobos
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 15:02
CPC Classifications
B; B01; B01J; B01J20/00; B01J20/22; B01J20/26; B01J20/261; B01J20/28; B01J20/28002; B01J20/28004; B01J20/28014; B01J20/28016; B01J20/28033; B01J20/281; B01J20/286; B01J20/30; B01J20/3092; B01J20/32; B01J20/3202; B01J20/3206; B01J20/3208; B01J20/321; B01J20/3214; B01J20/3217; B01J20/3219; B01J20/3231; B01J20/3242; B01J20/3244; B01J20/3246; B01J20/3248; B01J20/3251; B01J20/3268; B01J20/3272; B01J20/3274; B01J20/34; B01J20/3425; B01J20/345; C; C12; C12N; C12N11/00; C12N11/02; C12N11/08; C12N11/082; C12N15/00; C12N15/09; C12N15/10; C12N15/1003; C12N15/1006; C12Q; C12Q1/00; C12Q1/001; C12Q1/002; C12Q1/003; C12Q1/58; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/544; G01N33/545; B01J2220/00; B01J2220/40; B01J2220/49; B01J2220/50; B01J2220/54; B01J2220/58; Y; Y10; Y10S; Y10S435/00; Y10S435/803; Y10S435/814; Y10S435/815; Y10S435/817; Y10S436/00; Y10S436/824; Y10S530/00; Y10S530/81; Y10S530/812; Y10S530/815; Y10S530/816
Claims Analysis
Total Claims: 29
Claim 1
Independent Claim
72 words
A solid support containing an attached ligand or binder for the ligand consisting essentially of: (A) a chemically inert, water immiscible solid perfluorocarbon polymer carrier having low nonspecific binding to a ligand or binder for the ligand; (B) a highly fluorinated isocyanate-modified ligand or binder for the ligand securely but reversibly attached to the surface of said carrier through said highly fluorinated isocyanate; and (C) a nonionic fluorosurfactant coating on said carrier.
Claim 2
Dependent Claim
72 words
A solid support containing an attached ligand or binder for the ligand consisting essentially of: (A) a chemically inert, water immiscible solid perfluorocarbon polymer carrier having low nonspecific binding to a ligand or binder for the ligand; (B) a highly fluorinated isocyanate-modified ligand or binder for the ligand securely but reversibly attached to the surface of said carrier through said highly fluorinated isocyanate; and (C) a nonionic fluorosurfactant coating on said carrier.
Claim 3
Dependent Claim
19 words
The support of claim 1, wherein said ligand is selected from the group consisting of nucleic acid, and dye.
Claim 4
Dependent Claim
23 words
The support of claim 1, wherein said binder for the ligand is selected from the group consisting of antibody, enzyme and nucleic acid.
Claim 5
Dependent Claim
37 words
The solid support of claim 1 wherein the highly fluorinated isocyanate is a compound of the formula R.sub.F --CH.sub.2 CH.sub.2 CH.sub.2 NCO wherein R F is a linear, branched or carbocyclic perfluorinated radical containing 1-20 carbon atoms.
Claim 6
Dependent Claim
15 words
The solid support of claim 4 wherein R F is F(CF 2 ) 8 .
Claim 7
Dependent Claim
20 words
The support of claim 1, wherein the carrier is selected from the group consisting of polytetrafluoroethylene, polyvinylfluoride and polyvinylidene difluoride.
Claim 8
Dependent Claim
11 words
The support of claim 1, wherein said ligand is a hapten.
Claim 9
Dependent Claim
11 words
The support of claim 1, wherein said ligand is an antigen.
Claim 10
Dependent Claim
20 words
The support of claim 1, wherein said ligand is selected from the group consisting of enzyme substrates, effectors and inhibitors.
Claim 11
Dependent Claim
23 words
The support of claim 1, wherein said binder for the ligand is a binding protein or a synthetic mimic of a binding protein.
Claim 12
Dependent Claim
82 words
A bioaffinity separation process comprising the steps of: (A) forming a solid affinity support by (a) attaching a highly fluorinated isocyanate-modified ligand or binder for the ligand to the surface of a solid perfluorocarbon carrier through said highly fluorinated isocyanate and (b) contacting the product of step (a) with a nonionic fluorosurfactant to form an affinity support; and (B) capturing a binder or ligand, complementary to the ligand or binder attached to the carrier from a mixture using said solid affinity support.
Claim 13
Dependent Claim
82 words
A bioaffinity separation process comprising the steps of: (A) forming a solid affinity support by (a) attaching a highly fluorinated isocyanate-modified ligand or binder for the ligand to the surface of a solid perfluorocarbon carrier through said highly fluorinated isocyanate and (b) contacting the product of step (a) with a nonionic fluorosurfactant to form an affinity support; and (B) capturing a binder or ligand, complementary to the ligand or binder attached to the carrier from a mixture using said solid affinity support.
Claim 14
Dependent Claim
19 words
The process of claim 11, wherein said ligand is selected from the group consisting of nucleic acid, and dye.
Claim 15
Dependent Claim
23 words
The process of claim 11, wherein said binder for the ligand is selected from the group consisting of antibody, enzyme and nucleic acid.
Claim 16
Dependent Claim
11 words
The process of claim 11, wherein said ligand is a hapten.
Claim 17
Dependent Claim
11 words
The process of claim 11, wherein said ligand is an antigen.
Claim 18
Dependent Claim
20 words
The process of claim 11, wherein said ligand is selected from the group consisting of enzyme substrates, effectors and inhibitors.
Claim 19
Dependent Claim
23 words
The process of claim 11, wherein said binder for the ligand is a binding protein or a synthetic mimic of a binding protein.
Claim 20
Dependent Claim
59 words
An immobilized enzyme system consisting essentially of: (A) a chemically inert, water immiscible solid perfluorocarbon polymer carrier having low nonspecific binding to a ligand or binder for the ligand; (B) a highly fluorinated isocyanate-modified enzyme attached securely but reversibly to the surface of said carrier through said highly fluorinated isocyanate; and (C) a nonionic fluorosurfactant coating on said carrier.
Claim 21
Dependent Claim
59 words
An immobilized enzyme system consisting essentially of: (A) a chemically inert, water immiscible solid perfluorocarbon polymer carrier having low nonspecific binding to a ligand or binder for the ligand; (B) a highly fluorinated isocyanate-modified enzyme attached securely but reversibly to the surface of said carrier through said highly fluorinated isocyanate; and (C) a nonionic fluorosurfactant coating on said carrier.
Claim 22
Dependent Claim
89 words
A process of preparing a solid support containing an attached ligand or binder for the ligand comprising the steps of: (A) modifying a ligand or binder for the ligand by attaching a highly fluorinated isocyanate anchor group to said ligand or binder for the ligand; (B) contacting a solid perfluorocarbon polymer with the highly fluorinated isocyanate-modified ligand or binder for the ligand to attach the ligand or binder to the polymer through said highly fluorinated isocyanate, and (C) contacting the product formed the step (B) with a nonionic fluorosurfactant.
Claim 23
Dependent Claim
89 words
A process of preparing a solid support containing an attached ligand or binder for the ligand comprising the steps of: (A) modifying a ligand or binder for the ligand by attaching a highly fluorinated isocyanate anchor group to said ligand or binder for the ligand; (B) contacting a solid perfluorocarbon polymer with the highly fluorinated isocyanate-modified ligand or binder for the ligand to attach the ligand or binder to the polymer through said highly fluorinated isocyanate, and (C) contacting the product formed the step (B) with a nonionic fluorosurfactant.
Claim 24
Dependent Claim
19 words
The process of claim 19, wherein the ligand is selected from the group consisting of nucleic acid, and dye.
Claim 25
Dependent Claim
23 words
The process of claim 19, wherein said binder for the ligand is selected from the group consisting of antibody, enzyme and nucleic acid.
Claim 26
Dependent Claim
11 words
The process of claim 19, wherein said ligand is a hapten.
Claim 27
Dependent Claim
11 words
The process of claim 19, wherein said ligand is an antigen.
Claim 28
Dependent Claim
20 words
The process of claim 19, wherein said ligand is selected from the group consisting of enzyme substrates, effectors and inhibitors.
Claim 29
Dependent Claim
23 words
The process of claim 19, wherein said binder for the ligand is a binding protein or a synthetic mimic of a binding protein.
References Cited (Backward Citations)
US3843443A
Cited
US4119897A
Cited
US4177038A
Cited
EP0011504A1
Cited
US4317879A
Cited
US4412000A
Cited
US4619904A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1993-09-03 | 1994-03-05 | 1994-09-04 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1997-09-03 | 1998-03-05 | 1998-09-04 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2001-09-03 | 2002-03-05 | 2002-09-04 | $7700.00 | Window Closed / Lapsed |