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US5000922A Expired / Lapsed US ⏱️ 0.0 years remaining

Sample filtration, separation and dispensing device

Assignee: Bio Chem Laboratory Systems Inc
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Filing: 1988-05-12
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Granted: 1991-03-19
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Expires: 2008-05-12
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Abstract

Abstract An apparatus for dispensing a predetermined amount of a sample into a fluid. The apparatus comprises a chamber which may be inserted into a cuvette. The chamber comprises a membrane or filter ball for dispensing a predetermined amount of a sample into a fluid upon contact of the membrane or filter ball and the sample by the fluid. The fluid is brought into contact with the sample by vortexing the fluid with a vortex mixer.

Inventors

  • 👤 Jon Turpen

Bibliographic Details

Application Number —
Jurisdiction US
Data Source google_patents
Last Synchronized 2026-11-07 17:14
CPC Classifications
B; B01; B01L; B01L3/00; B01L3/50; B01L3/508; B01D; B01D61/00; B01D61/14; B01D61/18; Y; Y10; Y10T; Y10T436/00; Y10T436/25; Y10T436/25375; Y10T436/255

Claims Analysis

Total Claims: 16
Claim 1
Independent Claim 193 words
An article of manufacture, comprising: a chamber for holding a sample, said chamber including means defining an entrance opening and means defining an exit opening, said chamber also comprising: a first membrane disposed within said chamber for particulate and/or cell filtration from a sample; and a second membrane disposed below said first membrane and above said exit opening for metering a predetermined amount of sample out of said exit opening into a fluid upon contact of said second membrane by a fluid; a cuvette, said cuvette comprising a top portion and a bottom portion, for containing a fluid in the bottom portion of said cuvette, and said cuvette receiving said chamber, said chamber having at least one clip means extending outwardly from the top of said chamber and engaging the top portion of said cuvette, whereby said chamber is fixedly suspended within said cuvette above a fluid upon engagement of said top portion of said cuvette with said a least one clip means; and seal means within said cuvette, said seal means disposed above a fluid and said seal means being broken by said chamber upon insertion of said chamber into said cuvette.
Claim 2
Dependent Claim 193 words
An article of manufacture, comprising: a chamber for holding a sample, said chamber including means defining an entrance opening and means defining an exit opening, said chamber also comprising: a first membrane disposed within said chamber for particulate and/or cell filtration from a sample; and a second membrane disposed below said first membrane and above said exit opening for metering a predetermined amount of sample out of said exit opening into a fluid upon contact of said second membrane by a fluid; a cuvette, said cuvette comprising a top portion and a bottom portion, for containing a fluid in the bottom portion of said cuvette, and said cuvette receiving said chamber, said chamber having at least one clip means extending outwardly from the top of said chamber and engaging the top portion of said cuvette, whereby said chamber is fixedly suspended within said cuvette above a fluid upon engagement of said top portion of said cuvette with said a least one clip means; and seal means within said cuvette, said seal means disposed above a fluid and said seal means being broken by said chamber upon insertion of said chamber into said cuvette.
Claim 3
Dependent Claim 26 words
The article of claim 1, and further comprising means for exposing a predetermined area of a surface of said second membrane to contact with a fluid.
Claim 4
Dependent Claim 33 words
The article of claim 2 wherein said means for exposing a predetermined area of a surface of said second membrane to contact with fluid comprises a metering plate disposed beneath said second membrane.
Claim 5
Dependent Claim 16 words
The article of claim 1 and further comprising a cap means disposed above said entrance opening.
Claim 6
Dependent Claim 20 words
The article of claim 1 and further comprising a third membrane disposed between said first membrane and said second membrane.
Claim 7
Dependent Claim 40 words
The article of claim 5 wherein said second membrane is impregnated with a first reagent and said third membrane is impregnated with a second reagent, wherein a first reagent and a second reagent react with a sample and a fluid.
Claim 8
Dependent Claim 24 words
The article of claim 1 wherein said second membrane is impregnated with at least one reagent for reacting with a sample and a fluid.
Claim 9
Dependent Claim 64 words
The article of claim 1, and further comprising cutting means attached to said chamber and located beneath said second membrane of said chamber, said cutting means breaking said seal means upon insertion of said chamber into said cuvette, said cutting means also including means defining at least one opening to accommodate the passage of a fluid through said cutting means to said second membrane.
Claim 10
Dependent Claim 179 words
An article of manufacturing comprising: a chamber for holding a sample, said chamber including means defining an entrance opening and means defining an exit opening, said chamber also comprising a filter means for particulate and/or cell filtration from a sample, said filter means also metering a predetermined amount of sample out of said exit opening into a fluid upon contact of said filter means by a fluid; a cuvette, said cuvette comprising a top portion and a bottom portion, said cuvette containing a fluid in the bottom portion of said cuvette, and said cuvette receiving said chamber, said chamber having at least one clip means extending outwardly from the top of said chamber and engaging the top portion of said cuvette, whereby said chamber is fixedly suspended within said cuvette above a fluid upon engagement of said top portion of said cuvette with said at least one clip means; and seal means within said cuvette, said seal means disposed above a fluid and said seal means being broken by said chamber upon insertion of said chamber into said cuvette.
Claim 11
Dependent Claim 179 words
An article of manufacturing comprising: a chamber for holding a sample, said chamber including means defining an entrance opening and means defining an exit opening, said chamber also comprising a filter means for particulate and/or cell filtration from a sample, said filter means also metering a predetermined amount of sample out of said exit opening into a fluid upon contact of said filter means by a fluid; a cuvette, said cuvette comprising a top portion and a bottom portion, said cuvette containing a fluid in the bottom portion of said cuvette, and said cuvette receiving said chamber, said chamber having at least one clip means extending outwardly from the top of said chamber and engaging the top portion of said cuvette, whereby said chamber is fixedly suspended within said cuvette above a fluid upon engagement of said top portion of said cuvette with said at least one clip means; and seal means within said cuvette, said seal means disposed above a fluid and said seal means being broken by said chamber upon insertion of said chamber into said cuvette.
Claim 12
Dependent Claim 26 words
The article of claim 9, and further comprising means for exposing a predetermined area of a surface of said filter means to contact with a fluid.
Claim 13
Dependent Claim 31 words
The article of claim 10 wherein said means for exposing a predetermined area of said filter means to contact with a fluid comprises a metering plate disposed beneath said filter means.
Claim 14
Dependent Claim 16 words
The article of claim 9, and further comprising a cap means disposed above said entrance opening.
Claim 15
Dependent Claim 24 words
The article of claim 9 wherein said filter means is impregnated with at least one reagent for reacting with a sample and a fluid.
Claim 16
Dependent Claim 64 words
The article of claim 9, and further comprising cutting means attached to said chamber and located beneath said filter means of said chamber, said cutting means breaking said seal means upon insertion of said chamber into said cuvette said cutting means also including means defining at least one opening to accommodate the passage of a fluid through said cutting means to said filter means.

References Cited (Backward Citations)

US589648A Cited
US4294594A Cited
US4426295A Cited
US4600507A Cited
US4632901A Cited
US4696797A Cited

USPTO Annuity & Maintenance Windows

Annuity Stage Window Opens Payment Deadline Surcharge Period Until Estimated Fee Status
3.5 Years (Year 4) 1994-03-18 1994-09-17 1995-03-19 $2000.00 Window Closed / Lapsed
7.5 Years (Year 8) 1998-03-18 1998-09-17 1999-03-19 $3760.00 Window Closed / Lapsed
11.5 Years (Year 12) 2002-03-18 2002-09-17 2003-03-19 $7700.00 Window Closed / Lapsed

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