US5037764A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for determination of an analyte and reagent therefor
Assignee: Roche Diagnostics GmbH
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Filing: 1987-07-16
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Granted: 1991-08-06
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Expires: 2007-07-16
Abstract
Abstract The present invention provides a process for the detection of an analyte, wherein a sample containing the analyte is incubated either with a labelled binding partner and the immobilized analyte or an immobilized analyte analogue or with the labelled analyte or analyte analogue and an immobilized binding partner, the binding partner displaying towards the immobilized or labelled analyte or analyte analogue a higher affinity than towards the free analyte, and the labelled binding partner or the labelled analyte or the labelled analyte analogue is present in insufficiency compared with the concentration of the analyte to be detected. The present invention also provides an agent for carrying out this process, wherein it contains either the immobilized analyte or an immobilized analyte analogue and a labelled binding partner or the labelled analyte or a labelled analyte analogue and an immobilized binding partner, as well as optionally an appropriate buffer system and further conventionally used adjuvants, the binding partner displaying towards the immobilized or labelled analyte or analyte analogue a higher affinity than towards the free analyte.
Inventors
- 👤 Hans-Erich Wilk
- 👤 Helmut Freitag
- 👤 Josef Burg
- 👤 Johann Berger
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 11:18
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/74; G01N33/78; G01N33/543; G01N33/54306
Claims Analysis
Total Claims: 24
Claim 1
Independent Claim
128 words
Process for detection of a free analyte in a liquid sample comprising incubating said sample with a labeled binding partner for said analyte and one of an immobilized analyte or immobilized analyte analogue, or incubating said sample with one of a labeled analyte or labeled analyte analogue and an immobilized binding partner for said analyte, wherein said binding partner has an affinity for immobilized and labeled analyte or immobilized and labeled analyte analogue greater than affinity for said free analyte, said labeled binding partner, labeled analyte or labeled analyte analogue being present in an amount less than the amount of free analyte in said sample, separating immobilized complex from said liquid and measuring label in either immobilized or liquid phase as a measure of analyte in said sample.
Claim 2
Dependent Claim
128 words
Process for detection of a free analyte in a liquid sample comprising incubating said sample with a labeled binding partner for said analyte and one of an immobilized analyte or immobilized analyte analogue, or incubating said sample with one of a labeled analyte or labeled analyte analogue and an immobilized binding partner for said analyte, wherein said binding partner has an affinity for immobilized and labeled analyte or immobilized and labeled analyte analogue greater than affinity for said free analyte, said labeled binding partner, labeled analyte or labeled analyte analogue being present in an amount less than the amount of free analyte in said sample, separating immobilized complex from said liquid and measuring label in either immobilized or liquid phase as a measure of analyte in said sample.
Claim 3
Dependent Claim
35 words
Process of claim 1, wherein said immobilized analyte binds to an insoluble carrier by a bridge member, and said binding partner has greater affinity toward said bridge member-bound immobilized analyte than toward said free analyte.
Claim 4
Dependent Claim
15 words
Process of claim 1, wherein said analyte analogue is structurally similar to said free analyte.
Claim 5
Dependent Claim
15 words
Process of claim 1, wherein said analyte analogue is a portion of said free analyte.
Claim 6
Dependent Claim
28 words
Process of claim 1, wherein said labeled binding partner, labeled analyte or labeled analyte analogue and said free analyte are in a ratio of from 1:1 to 1:10000.
Claim 7
Dependent Claim
13 words
Process of claim 1, wherein said incubating steps are carried out in sequence.
Claim 8
Dependent Claim
12 words
Process of claim 1, wherein said incubating steps are carried out simultaneously.
Claim 9
Dependent Claim
12 words
Process of claim 1, wherein said analyte analogue is an anti-idiotype antibody.
Claim 10
Dependent Claim
11 words
Process of claim 1, wherein said binding partner is a hapten.
Claim 11
Dependent Claim
11 words
Process of claim 1, wherein said binding partner is an antibody.
Claim 12
Dependent Claim
11 words
Process of claim 10, wherein said antibody is a monoclonal antibody.
Claim 13
Dependent Claim
11 words
Process of claim 10, wherein said antibody is a Fab fragment.
Claim 14
Dependent Claim
64 words
Reagent for detecting a free analyte in a liquid sample, comprising one of an immobilized analyte or immobilized analyte analogue and a labeled binding partner for said free analyte or one of a labeled analyte or labeled analyte analogue and an immobilized binding partner, said binding partner having an affinity for labeled or immobilized analyte or analyte analogue greater than for said free analyte.
Claim 15
Dependent Claim
64 words
Reagent for detecting a free analyte in a liquid sample, comprising one of an immobilized analyte or immobilized analyte analogue and a labeled binding partner for said free analyte or one of a labeled analyte or labeled analyte analogue and an immobilized binding partner, said binding partner having an affinity for labeled or immobilized analyte or analyte analogue greater than for said free analyte.
Claim 16
Dependent Claim
9 words
Reagent of claim 13, further comprising a buffer system.
Claim 17
Dependent Claim
10 words
Reagent of claim 13, further comprising one or more adjuvants.
Claim 18
Dependent Claim
15 words
Reagent of claim 13, further comprising one or more substances for determining said labeled material.
Claim 19
Dependent Claim
23 words
Reagent of claim 13, wherein said reagent is in the form of a kit containing separate portions of each component of said reagent.
Claim 20
Dependent Claim
11 words
Reagent of claim 17, wherein said components are in solution form.
Claim 21
Dependent Claim
13 words
Reagent of claim 17, wherein said components are in the form of carriers.
Claim 22
Dependent Claim
11 words
Reagent of claim 17, wherein said components are in powder form.
Claim 23
Dependent Claim
11 words
Reagent of claim 17, wherein said components are in tablet form.
Claim 24
Dependent Claim
9 words
Reagent of claim 17, wherein said components are lyophilisates.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1994-08-05 | 1995-02-04 | 1995-08-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1998-08-05 | 1999-02-04 | 1999-08-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2002-08-05 | 2003-02-04 | 2003-08-06 | $7700.00 | Window Closed / Lapsed |