US5082627A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Three dimensional binding site array for interfering with an electrical field
Assignee: BIOTRONIC SYSTEMS Corp 15225 SHADY GROVE ROAD SUITE 306 ROCKVILLE MD 20850
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Filing: 1987-05-01
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Granted: 1992-01-21
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Expires: 2007-05-01
Abstract
Abstract A dielectric material of a capacitive affinity sensor has a three-dimensional molecular binding site array. A glass base is layered with a binding agent like silane from which a polymeric backbone like polylysine extends. The polymeric backbone is prepared to accept receptor molecules like cortisol hemisuccinate to bind a specific antibody. Such an array changes dielectric properties between the two electrodes of the capacitive affinity sensor to greatly enhance sensitivity of the sensor.
Inventors
- 👤 William D. Stanbro
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 22:25
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/02; G01N27/22; G01N27/221; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54366; G01N33/54373; G01N33/5438; Y; Y10; Y10S; Y10S435/00; Y10S435/819
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
95 words
An apparatus for detecting an analyte comprising: an electrode for generating an electrical field; a means for interfering with the electrical field comprising a biochemically active layer that comprises a plurality of polylysine backbones, and a silane for binding each polylysine backbone and extending each polylysine backbone into the electrical field; and a means for enhancing interference with the electrical field according to a concentration of an analyte, comprising a plurality of receptor molecules biospecific to the analyte and bound to and extending from each polylysine backbone, each receptor molecule being biospecific to the analyte.
Claim 2
Independent Claim
95 words
An apparatus for detecting an analyte comprising: an electrode for generating an electrical field; a means for interfering with the electrical field comprising a biochemically active layer that comprises a plurality of polylysine backbones, and a silane for binding each polylysine backbone and extending each polylysine backbone into the electrical field; and a means for enhancing interference with the electrical field according to a concentration of an analyte, comprising a plurality of receptor molecules biospecific to the analyte and bound to and extending from each polylysine backbone, each receptor molecule being biospecific to the analyte.
Claim 3
Dependent Claim
116 words
A capacitive affinity sensor for detecting an analyte comprising: a means for generating an electrical field comprising two electrodes having opposite polarities; a means for interfering with the electrical field comprising a dielectric material between the two electrodes, the dielectric material comprising a biochemically active layer of a plurality of polylysine backbones and a means for binding each polylysine backbone and extending each polylysine backbone into the electric field and a means for enhancing interference with the electrical field according to a concentration of an analyte, comprising a plurality of binding sites on each polylysine backbone, each binding site comprising a receptor molecule biospecific to the analyte and bound to and extending from a polylysine backbone.
Claim 4
Dependent Claim
116 words
A capacitive affinity sensor for detecting an analyte comprising: a means for generating an electrical field comprising two electrodes having opposite polarities; a means for interfering with the electrical field comprising a dielectric material between the two electrodes, the dielectric material comprising a biochemically active layer of a plurality of polylysine backbones and a means for binding each polylysine backbone and extending each polylysine backbone into the electric field and a means for enhancing interference with the electrical field according to a concentration of an analyte, comprising a plurality of binding sites on each polylysine backbone, each binding site comprising a receptor molecule biospecific to the analyte and bound to and extending from a polylysine backbone.
References Cited (Backward Citations)
US4072576A
Cited
US4233402A
Cited
US4453126A
Cited
JPS5533449A
Cited
US4490216A
Cited
US4571543A
Cited
US4740468A
Cited
US4637861A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1995-01-20 | 1995-07-22 | 1996-01-21 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1999-01-20 | 1999-07-22 | 2000-01-21 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2003-01-20 | 2003-07-22 | 2004-01-21 | $7700.00 | Window Closed / Lapsed |