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US5116762A
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Method for determining the concentration of fructosamine using a standard solution and method of calibrating the standard solution
Assignee: Roche Diagnostics GmbH
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Filing: 1989-07-10
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Granted: 1992-05-26
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Expires: 2009-07-10
Abstract
Abstract The present invention provides a process for the determination of fructosamine in body fluids using as a standard, a solution which contains a peptide or protein, the amino acid units of which consist of at least 25% of lysine and/or ornithine and is present in glycosylated form. The present invention also provides a standard solution for the determination of fructosamine containing a peptide or protein, the amino acid unit content of which consists of at least 25% lysine and/or ornithine and is present in glycosylated form, and a method of calibrating such a standard solution.
Inventors
- 👤 Bernd Vogt
- 👤 Hans-Dieter Lessmann
- 👤 Christian Klein
- 👤 Wolfgang Treiber
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 22:11
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/96; G01N33/66; Y; Y10; Y10S; Y10S436/00; Y10S436/904; Y10T; Y10T436/00; Y10T436/10; Y10T436/104998; Y10T436/105831; Y10T436/14; Y10T436/142222; Y10T436/143333; Y10T436/144444
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
102 words
A method for the determination of the concentration of fructosamine in a body fluid sample comprising measuring a characteristic of the body fluid related to fructosamine, and using a glucose-free peptide or protein-containing solution having a pH value range of less than 1 to 4 as a standard solution for calibration wherein the peptide or protein contains amino acid units comprising at least 25% of lysine, ornithine or mixtures thereof and the protein or peptide is glycosylated and wherein the characteristic of the body fluid sample solution is compared to the standard solution to determine the fructosamine concentration of the body fluid.
Claim 2
Independent Claim
102 words
A method for the determination of the concentration of fructosamine in a body fluid sample comprising measuring a characteristic of the body fluid related to fructosamine, and using a glucose-free peptide or protein-containing solution having a pH value range of less than 1 to 4 as a standard solution for calibration wherein the peptide or protein contains amino acid units comprising at least 25% of lysine, ornithine or mixtures thereof and the protein or peptide is glycosylated and wherein the characteristic of the body fluid sample solution is compared to the standard solution to determine the fructosamine concentration of the body fluid.
Claim 3
Dependent Claim
21 words
The method of claim 1, comprising using polylysine, polyornithine or a co-polymer of lysine and ornithine as the peptide or protein.
Claim 4
Independent Claim
28 words
The method of claims 1 or 2 comprising using a peptide or protein-containing solution wherein the peptide or protein has a molecular weight of 1000 to 300,000 daltons.
Claim 5
Dependent Claim
16 words
The method of claim 3 comprising using a peptide or protein of 1000-150,000 daltons molecular weight.
Claim 6
Dependent Claim
19 words
The method of claim 1 or 2 comprising using human serum as a base solution for the standard solution.
Claim 7
Dependent Claim
27 words
The method of claim 1 comprising using a peptide- or protein wherein 5 to 50% of all amino side groups of the peptide or protein are glycosylated.
Claim 8
Dependent Claim
27 words
The method of claim 6, comprising using a peptide or protein wherein 5 to 35% of all amino side groups of the peptide or protein are glycosylated.
Claim 9
Dependent Claim
14 words
The method of claim 1 comprising using a standard solution having a buffer system.
Claim 10
Independent Claim
108 words
A method for the calibration of a standard for the determination of fructosamine in body fluids comprising analyzing a series of known concentrations of a standard solution by a test for fructosamine in order to compare those results to a similar test result for a body fluid wherein the body fluid test result is compared to that of the standard in order to determine the fructosamine content of the body fluid and wherein the standard solution has a pH value of less than 1 to 4 and is a glycosylated peptide or protein with an amino acid content comprising at lest 25% of lysine, ornithine or mixtures thereof.
Claim 11
Independent Claim
108 words
A method for the calibration of a standard for the determination of fructosamine in body fluids comprising analyzing a series of known concentrations of a standard solution by a test for fructosamine in order to compare those results to a similar test result for a body fluid wherein the body fluid test result is compared to that of the standard in order to determine the fructosamine content of the body fluid and wherein the standard solution has a pH value of less than 1 to 4 and is a glycosylated peptide or protein with an amino acid content comprising at lest 25% of lysine, ornithine or mixtures thereof.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1995-05-26 | 1995-11-25 | 1996-05-26 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1999-05-26 | 1999-11-25 | 2000-05-26 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2003-05-26 | 2003-11-25 | 2004-05-26 | $7700.00 | Window Closed / Lapsed |