Patent US733142A successfully refreshed from Google Patents.
Patent US5147609A successfully refreshed from Google Patents.
US5147609A
Expired / Lapsed
US
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Assay element
Assignee: P B DIAGNOSTIC SYSTEMS Inc A CORP OF
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Filing: 1989-05-19
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Granted: 1992-09-15
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Expires: 2009-05-19
Abstract
Abstract There is described an assay element suitable for use in an automated analytical test instrument for assaying a fluid sample. The element includes a thin porous member possessing a high degree of capillarity such as a fibrous mesh pad supported within a guide defined by surfaces contiguous the porous member. The dimensions of the porous member and its degree of capillarity are such as to provide for capillary transport of fluid through the member. A fluid dispenser and a fluid collecting chamber are disposed contiguous to opposed ends of the porous member. The fluid dispenser is formed as a well with a port at the bottom of the well, the port being contiguous the porous member and having a ridge extending along a perimeter of the port. The ridge protrudes into the porous member a distance sufficient for entraining fluid present in the well to propagate within the member rather than along an interface between a surface of the member and guide surfaces which hold it in place. Reservoirs may also be provided in the housing for storing reagents, mixing fluids and diluting samples so as to provide a self contained assay element. Optionally, and preferably, there may be an opening in the housing to provide fluid access directly to the central area of the porous member.
Inventors
- 👤 Gerd Grenner
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 13:30
CPC Classifications
B; B01; B01L; B01L3/00; B01L3/50; B01L3/502; B01L3/5023; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54366; B01L2200/00; B01L2200/02; B01L2200/026; B01L2200/027; B01L2300/00; B01L2300/08; B01L2300/0809; B01L2300/0825; B01L2400/00; B01L2400/04; B01L2400/0403; B01L2400/0406; Y; Y10; Y10S; Y10S435/00; Y10S435/97; Y10S436/00; Y10S436/807; Y10S436/808
Claims Analysis
Total Claims: 21
Claim 1
Independent Claim
127 words
An assay element comprising a housing including a porous member arranged between top and bottom guide surfaces, a chamber for dispensing fluid to an end portion of said porous member and a chamber for receiving fluid which leaves said porous member, said fluid dispensing chamber and said fluid receiving chamber being disposed contiguous opposed ends of said porous member, wherein said fluid dispensing chamber includes a well having a port in the bottom thereof contiguous said porous member and opening to said porous member for conducting fluid from said well to said porous member; and a ridge encircling the perimeter of said port and protruding into and compressing said porous member for entraining fluid from said fluid dispensing chamber to propagate into and within said porous member.
Claim 2
Dependent Claim
127 words
An assay element comprising a housing including a porous member arranged between top and bottom guide surfaces, a chamber for dispensing fluid to an end portion of said porous member and a chamber for receiving fluid which leaves said porous member, said fluid dispensing chamber and said fluid receiving chamber being disposed contiguous opposed ends of said porous member, wherein said fluid dispensing chamber includes a well having a port in the bottom thereof contiguous said porous member and opening to said porous member for conducting fluid from said well to said porous member; and a ridge encircling the perimeter of said port and protruding into and compressing said porous member for entraining fluid from said fluid dispensing chamber to propagate into and within said porous member.
Claim 3
Dependent Claim
16 words
The assay element according to claim 1 wherein said porous member comprises a fibrous mesh material.
Claim 4
Dependent Claim
24 words
The assay element according to claim 2 wherein said fibrous mesh material has a thickness of from about 0.5 μm to about 2.5 μm.
Claim 5
Dependent Claim
16 words
The assay element according to claim 3 wherein said fibrous mesh material comprises nonwoven glass fibers.
Claim 6
Dependent Claim
66 words
The assay element according to claim 2 wherein said porous member has a longitudinal dimension extending from said fluid dispensing chamber to said fluid receiving chamber, said porous member having a transverse dimension lying in a plane of said port and extending perpendicular to said longitudinal dimension; and wherein a diameter of said port is smaller than said longitudinal dimension and smaller than said transverse dimension.
Claim 7
Dependent Claim
30 words
The assay element according to claim 5 wherein said port is symmetrically positioned on a center line of said porous member, said center line extending parallel to said longitudinal dimension.
Claim 8
Dependent Claim
30 words
The assay element according to claim 1 wherein said ridge has a taper on a side thereof away from said port, the taper extending to one of said guide surfaces.
Claim 9
Dependent Claim
102 words
The assay element according to claim 1 wherein a side of said ridge facing said port extends directly into said porous member, said ridge having a taper of a side thereof facing away from said port, said taper extending to one of said guide surfaces; and wherein said ridge has a depth of penetration into said porous member measured from said one guide surface, said ridge having a width measured from an edge of said port to an intersection of said taper with said one surface, said ridge width being larger than said depth of penetration by a factor of approximately 2.5.
Claim 10
Dependent Claim
53 words
The assay element according to claim 8 wherein the width of said ridge is approximately 0.55 mm, the penetration depth of said ridge is approximately 0.2 mm, said taper has an angle of approximately 30 degrees measured relative to said one guide surface, and wherein a tip portion of said ridge is rounded.
Claim 11
Dependent Claim
14 words
The assay element according to claim 8 wherein said port has a rectangular shape.
Claim 12
Dependent Claim
24 words
The assay element according to claim 1 and further comprising an opening disposed in said housing above a central portion of said porous member.
Claim 13
Dependent Claim
27 words
The assay element according to claim 11 and further including at least one reservoir integrally formed within said housing for storing fluid reagent required for an assay.
Claim 14
Dependent Claim
29 words
The assay element according to claim 12 and further including a reservoir integrally formed in said housing for mixing fluid reagents and/or a fluid sample required for said assay.
Claim 15
Dependent Claim
36 words
The assay element according to claim 11 and further including a window disposed in said housing contiguous the bottom surface of said porous member, said window permitting electromagnetic radiation to be directed onto said porous member.
Claim 16
Dependent Claim
24 words
The assay element according to claim 11 and further including an opening disposed in said housing contiguous the bottom surface of said porous member.
Claim 17
Dependent Claim
33 words
The assay element according to claim 11 wherein said fluid receiving chamber disposed contiguous one end of said porous member includes a fluid absorbing material disposed in said chamber contiguous said porous member.
Claim 18
Dependent Claim
102 words
The assay element according to claim 16 wherein a side of said ridge facing said port extends directly into the porous member, said ridge having a taper of a side thereof facing away from said port, said taper extending to one of said guide surfaces; and wherein said ridge has a depth of penetration into the porous member measured from said one guide surface, said ridge having a width measured from an edge of said port to an intersection of said taper within said one surface, said ridge width being larger than said depth of penetration by a factor of approximately 2.5.
Claim 19
Dependent Claim
54 words
The assay element according to claim 17 wherein said guide surfaces define a guide enclosing said porous member and extending longitudinally along said porous member, said guide having a depth, as measured in a direction perpendicular to the plane of said port, in the range of from about 0.08 mm to about 0.14 mm.
Claim 20
Dependent Claim
33 words
The assay element according to claim 1 wherein said fluid receiving chamber disposed contiguous one end of said porous member includes a fluid absorbing material disposed in said chamber contiguous said porous member.
Claim 21
Dependent Claim
27 words
The assay element according to claim 1 wherein said ridge protrudes into said porous member about one-half of the distance between the top and bottom surfaces thereof.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1995-09-15 | 1996-03-16 | 1996-09-15 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1999-09-15 | 2000-03-16 | 2000-09-15 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2003-09-15 | 2004-03-16 | 2004-09-15 | $7700.00 | Window Closed / Lapsed |