Patent US36291A successfully refreshed from Google Patents.
Patent US5185444A successfully refreshed from Google Patents.
US5185444A
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Uncharged morpolino-based polymers having phosphorous containing chiral intersubunit linkages
Assignee: Sarepta Therapeutics Inc
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Filing: 1991-11-21
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Granted: 1993-02-09
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Expires: 2011-11-21
Abstract
Abstract A polymer composition is disclosed composed of morpholino subunit structures linked together by uncharged, chiral linkages, one-three atoms in length. These chiral linkages join the morpholino nitrogen of one subunit to the 5' exocyclic carbon of an adjacent subunit. Each subunit contains a purine or pyrimidine base-pairing moiety effective to bind by hydrogen bonding to a specific base or base-pair in a target polynucleotide.
Inventors
- 👤 James E. Summerton
- 👤 Dwight D. Weller
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 07:08
CPC Classifications
C; C07; C07H; C07H21/00; A; A61; A61K; A61K47/00; A61K47/50; A61K47/51; A61K47/54; A61K47/545; A61K47/548; A61K47/56; A61K47/59; A61K47/60; A61K47/605; C07K; C07K14/00; C07K14/001; C07K14/003; C08; C08G; C08G79/00; C08G79/02; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6813; C12Q1/6816; C12Q1/682; B; B01; B01J; B01J2219/00; B01J2219/00274; B01J2219/00718; B01J2219/0072
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
69 words
A polymer composition comprised of morpholino subunit structures of the form: ##STR2## where (i) the structures are linked together by uncharged phosphorous-containing, chiral linkages, one to three atoms long, joining the morpholino nitrogen of one subunit to the 5', exocyclic carbon of an adjacent subunit, and (ii) P i is a purine or pyrimidine base-pairing moiety effective to bind by base-specific hydrogen bonding to a base in a polynucleotide.
Claim 2
Dependent Claim
69 words
A polymer composition comprised of morpholino subunit structures of the form: ##STR2## where (i) the structures are linked together by uncharged phosphorous-containing, chiral linkages, one to three atoms long, joining the morpholino nitrogen of one subunit to the 5', exocyclic carbon of an adjacent subunit, and (ii) P i is a purine or pyrimidine base-pairing moiety effective to bind by base-specific hydrogen bonding to a base in a polynucleotide.
Claim 3
Dependent Claim
28 words
The composition of claim 1, wherein P i is selected from the group consisting of: ##STR3## where X is H, CH 3 , F, Cl, Br, or I.
Claim 4
Dependent Claim
19 words
The composition of claim 1, wherein the linked structures have a form selected from the group consisting of: ##STR4##
Claim 5
Dependent Claim
82 words
The composition of claim 1, wherein the linkage is of the form: ##STR5## where P j is a purine or pyrimidine base-pairing moiety effective to bind by base-specific hydrogen bonding to a base in a polynucleotide; and, X is F, CH 2 R, O--CH 2 R, S--CH 2 R, or NR 1 R 2 ; and each of R, R 1 and R 2 is H, CH 3 , or other moiety that does not interfere with said base specific hydrogen bonding.
Claim 6
Dependent Claim
83 words
The composition of claim 1, wherein the linkage is of the form: ##STR6## where P j is a purine or pyrimidine base-pairing moiety effective to bind by base-specific hydrogen bonding to a base in a polynucleotide; and, X is F, CH 2 R, O--CH 2 R, S--CH 2 R, or NR 1 R 2 ; and each of R, R 1 and R 2 , is H, CH 3 , or other moiety that does not interfere with said base specific hydrogen bonding.
Claim 7
Dependent Claim
42 words
The composition of claim 1, wherein the linkage is of the form: ##STR7## where P j is a purine or pyrimidine base-pairing moiety effective to bind by base-specific hydrogen bonding to a base in a polynucleotide; and, Z is O or S.
Claim 8
Dependent Claim
42 words
The composition of claim 1, wherein the linkage is of the form: ##STR8## where P j is a purine or pyrimidine base-pairing moiety effective to bind by base-specific hydrogen bonding to a base in a polynucleotide; and, Z is O or S.
Claim 9
Dependent Claim
83 words
The composition of claim 1, wherein the linkage is of the form: ##STR9## where P j is a purine or pyrimidine base-pairing moiety effective to bind by base-specific hydrogen bonding to a base in a polynucleotide; and, X is F, CH 2 R, O--CH 2 R, S--CH 2 R, or NR 1 R 2 ; and each of R, R 1 and R 2 , is H, CH 3 , or other moeity which does not interfere with said base specific hydrogen bonding.
Claim 10
Dependent Claim
83 words
The composition of claim 1, wherein the linkage is of the form: ##STR10## where P j is a purine or pyrimidine base-pairing moiety effective to bind by base-specific hydrogen bonding to a base in a polynucleotide; and, X is F, CH 2 R, O--CH 2 R, S--CH 2 R, or NR 1 R 2 ; and each of R, R 1 and R 2 , is H, CH 3 , or other moeity which does not interfere with said base specific hydrogen bonding.
Claim 11
Dependent Claim
28 words
The composition of claim 1, which further includes a moiety at one or both termini which is effective to enhance the solubility of the molecules in aqueous medium.
Claim 12
Dependent Claim
16 words
The composition of claim 10, wherein the moiety at one or both termini is polyethylene glycol.
Claim 13
Dependent Claim
12 words
The composition of claim 1, composed of at least 3 morpholino subunits.
Claim 14
Dependent Claim
16 words
The composition of claim 1, wherein at least one of the P i is a 2,6-diaminopurine.
Claim 15
Dependent Claim
16 words
The composition of claim 1, wherein at least one of the P i is a 5-halouracil.
Claim 16
Dependent Claim
17 words
The composition of claim 1, wherein at least 70% of the P i are 2-amine containing purines.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1996-02-09 | 1996-08-10 | 1997-02-09 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2000-02-09 | 2000-08-10 | 2001-02-09 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2004-02-09 | 2004-08-10 | 2005-02-09 | $7700.00 | Window Closed / Lapsed |