Patent US5187103A successfully refreshed from Google Patents.
US5187103A
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US
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Colorimetric method and reagent for the assay of lithium in a test sample
Assignee: Siemens Healthcare Diagnostics Inc
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Filing: 1991-01-31
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Granted: 1993-02-16
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Expires: 2011-01-31
Abstract
Abstract A chromogenic cryptand found to be especially selective in the determination of lithium in blood of the general formula (I): ##STR1## where: n=1 or 2, R 1 and R 2 , same or different, are hydrogen, lower alkyl, lower alkenyl, or lower alkylidene; and Q is a chromogenic moiety capable of providing a detectable response upon complexation of said compound with lithium ion and has the structure: ##STR2## wherein: X is CH, C--OH or N; and Z is p-nitrophenylazo, 2,4-dinitrophenylazo, 2,4,6-trinitrophenylazo, p-nitrostyryl, p-benzoquinonemonoimino, bis-(4-dimethylaminophenyl) hydroxymethyl, 3-phenylisothiazolyl-5-azo, thiazolyl-5-azo; or isothiazolyl-5-azo. A reagent composition and synthesis of a preferred chromogenic cryptand are also disclosed.
Inventors
- 👤 Bronislaw P. Czech
- 👤 Eddy Chapoteau
- 👤 Anand Kumar
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 22:36
CPC Classifications
C; C07; C07D; C07D498/00; C07D498/02; C07D498/08; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/52; G01N33/84
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
74 words
A chromogenic cryptand having the structure: ##STR9## where: n=1 or 2, R 1 and R 2 , same or different, are hydrogen, lower alkyl, lower alkenyl, or lower alkylidene; and Q is a chromogenic moiety which provides a detectable response upon complexation of said compound with lithium ion and has the structure: ##STR10## wherein: X is CH, C-OH or N; and Z is p-nitrophenylazo, 2,4-dinitrophenylazo, 2,4,6-trinitrophenylazo, p-nitrostyryl, p-benzoquinonemonoimino, bis-(4-dimethylaminophenyl) hydroxymethyl, 3-phenylisothiazolyl-5-azo, thiazolyl-5-azo, or isothiazolyl-5-azo.
Claim 2
Dependent Claim
74 words
A chromogenic cryptand having the structure: ##STR9## where: n=1 or 2, R 1 and R 2 , same or different, are hydrogen, lower alkyl, lower alkenyl, or lower alkylidene; and Q is a chromogenic moiety which provides a detectable response upon complexation of said compound with lithium ion and has the structure: ##STR10## wherein: X is CH, C-OH or N; and Z is p-nitrophenylazo, 2,4-dinitrophenylazo, 2,4,6-trinitrophenylazo, p-nitrostyryl, p-benzoquinonemonoimino, bis-(4-dimethylaminophenyl) hydroxymethyl, 3-phenylisothiazolyl-5-azo, thiazolyl-5-azo, or isothiazolyl-5-azo.
Claim 3
Dependent Claim
24 words
The chromogenic cryptand of claim 1 wherein R 1 and R 2 are hydrogen, n is 1, X is COH and Z is p-nitrophenylazo.
Claim 4
Dependent Claim
41 words
A composition for detecting the presence of lithium ion in solution, said composition comprising the compound of one of claims 1-2, a water miscible organic solvent, and a buffer to adjust the pH of the reagent composition to at least 12.
Claim 5
Dependent Claim
25 words
The composition of claim 3 wherein the water miscible organic solvent is diethylene glycol monoethyl ether at a concentration less than 25% volume to volume.
Claim 6
Dependent Claim
9 words
The composition of claim 3 further including a surfactant.
Claim 7
Independent Claim
141 words
A method for selectively determining the presence of lithium ions in a test sample comprising the steps of: (a) contacting said test sample with a reagent composition including a compound which complexes selectivity to the test cation and being the formula: ##STR11## where: n=1 or 2, 1R 1 and R 2 , same or different, are hydrogen, lower alkyl, lower alkenyl, or lower alkylidene; and Q is a chromogenic moiety which provides a detectable response upon complexation of said compound with lithium ion and has the structure: ##STR12## wherein: X is CH, C-OH or N; and Z is p-nitrophenylazo, 2,4-dinitrophenylazo, 2,4,6-trinitrophenylazo, p-nitrostyryl, p-benzoquinonemonoimino, bis-(4-dimethylaminophenyl) hydroxymethyl, 3-phenylisothiazolyl-5-azo, thiazolyl-5-azo, or isothiazolyl-5-azo; (b) measuring the detectable response; and (c) comparing the response so detected with responses measured when said compound is reacted with a series of standard compositions containing known amounts of lithium ions.
Claim 8
Independent Claim
141 words
A method for selectively determining the presence of lithium ions in a test sample comprising the steps of: (a) contacting said test sample with a reagent composition including a compound which complexes selectivity to the test cation and being the formula: ##STR11## where: n=1 or 2, 1R 1 and R 2 , same or different, are hydrogen, lower alkyl, lower alkenyl, or lower alkylidene; and Q is a chromogenic moiety which provides a detectable response upon complexation of said compound with lithium ion and has the structure: ##STR12## wherein: X is CH, C-OH or N; and Z is p-nitrophenylazo, 2,4-dinitrophenylazo, 2,4,6-trinitrophenylazo, p-nitrostyryl, p-benzoquinonemonoimino, bis-(4-dimethylaminophenyl) hydroxymethyl, 3-phenylisothiazolyl-5-azo, thiazolyl-5-azo, or isothiazolyl-5-azo; (b) measuring the detectable response; and (c) comparing the response so detected with responses measured when said compound is reacted with a series of standard compositions containing known amounts of lithium ions.
Claim 9
Dependent Claim
23 words
The method of claim 6 wherein R 1 and R 2 are hydrogen, n is 1, X is COH and Z is p-nitrophenylazo.
Claim 10
Dependent Claim
31 words
The method of claim 6 wherein said reagent composition further includes a water miscible solvent and a buffer to maintain the pH of the reagent composition at about at least 12.
Claim 11
Dependent Claim
26 words
The method of claim 8 wherein the water miscible organic solvent is diethylene glycol monoethyl ether at a concentration of less than 25% volume to volume.
Claim 12
Dependent Claim
13 words
The method of claim 8 wherein the reagent composition further includes a surfactant.
References Cited (Backward Citations)
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| 3.5 Years (Year 4) | 1996-02-16 | 1996-08-17 | 1997-02-16 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2000-02-16 | 2000-08-17 | 2001-02-16 | $3760.00 | Window Closed / Lapsed |
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