US5278047A
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Method of analysis, reagent composition and use thereof for glucose determination
Assignee: Individual
•
Filing: 1990-04-24
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Granted: 1994-01-11
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Expires: 2010-04-24
Abstract
Abstract A method, a reagent composition and the use thereof for quantitative determination of total glucose in undiluted whole blood are described. A sample of undiluted whole blood is contacted with a reagent composition which is in dry form and comprises: glucose dehydrogenase (GDH); one or more substances from the group consisting of diaphorase, phenazine methosulphate, phenazine ethosulphate, phenazine phenosulphate and Meldola blue; one or more substances from the group consisting of NAD, NADP, thio-NAD, thio-NADP, nicotinamide-purine dinucleotide, nicotinamide-methylpurine dinucleotide, nicotinamide-2-chloro-methylpurine dinucleotide; one or more hemolysing substances from the group consisting of phospholipase, hemolysing saponins, and compounds of hydrophilic mono-, di- or tri-saccharides and aliphatic hydrocarbons having 10-16 carbon atoms; a redox indicator dye; and optionally mutarotase. A color change brought about by the reaction of the reagent with glucose in the undiluted whole blood is measured by transmission spectrophotometry.
Inventors
- 👤 Jan E. Lilja
- 👤 Sven-Erik L. Nilsson
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 22:49
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/54
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
148 words
A method to quantitatively determine the glucose concentration of a sample of undiluted whole blood, comprising contacting a sample of undiluted whole blood with a reagent in dry form in a microcuvette to obtain hemolyzed undiluted whole blood, the reagent comprising: glucose dehydrogenase (GDH); one or more substances from the group consisting of diaphorase, phenazine methosulphate, phenazine ethosulphate, phenazine phenosulphate and Meldola blue; one or more substances from the group consisting of NAD, NADP, thio-NAD, thio-NADP, nicotinamide-purine dinucleotide, nicotinamide-methylpurine dinucleo-tide and nicotinamide-2-chloro-methylpurine dinucleo-tide; one or more hemolyzing substances from the group consisting of phospholipase, hemolyzing saponins, compounds of hydrophilic mono-, di- or tri-saccharides and aliphatic hydrocarbons having 10-16 carbon atoms; and a redox indicator dye; and measuring by transmission spectrophotometry a color change brought about by the reaction of the reagent with glucose in the undiluted whole blood to quantitatively determine the glucose concentration of undiluted whole blood.
Claim 2
Independent Claim
148 words
A method to quantitatively determine the glucose concentration of a sample of undiluted whole blood, comprising contacting a sample of undiluted whole blood with a reagent in dry form in a microcuvette to obtain hemolyzed undiluted whole blood, the reagent comprising: glucose dehydrogenase (GDH); one or more substances from the group consisting of diaphorase, phenazine methosulphate, phenazine ethosulphate, phenazine phenosulphate and Meldola blue; one or more substances from the group consisting of NAD, NADP, thio-NAD, thio-NADP, nicotinamide-purine dinucleotide, nicotinamide-methylpurine dinucleo-tide and nicotinamide-2-chloro-methylpurine dinucleo-tide; one or more hemolyzing substances from the group consisting of phospholipase, hemolyzing saponins, compounds of hydrophilic mono-, di- or tri-saccharides and aliphatic hydrocarbons having 10-16 carbon atoms; and a redox indicator dye; and measuring by transmission spectrophotometry a color change brought about by the reaction of the reagent with glucose in the undiluted whole blood to quantitatively determine the glucose concentration of undiluted whole blood.
Claim 3
Dependent Claim
22 words
The method as claimed in claim 1, wherein the sample of undiluted whole blood is a volume of 0.1 to 0.001 ml.
Claim 4
Dependent Claim
94 words
A reagent composition to quantitatively determine the total glucose concentration of undiluted whole blood, wherein the reagent composition is in dry form and the reagent composition comprises: glucose dehydrogenase (GDH); one or more substances form the group consisting of diaphorase, phenazine methosulphate, phenazine, ethosulphate, phenazine phenosulphate and Meldola blue; one or more substances from the group consisting of NAD, NADP, thio-NAD, thio-NAD, nicotinamide-purine dinucleotide and nicotinamide methylpurine dinucleotide; one or more hemolyzing substances from the group consisting of phospholipase, hemolyzing saponins, saccharides and aliphatic hydrocarbons having 10-16 carbon atoms; and a redox indicator dye.
Claim 5
Dependent Claim
94 words
A reagent composition to quantitatively determine the total glucose concentration of undiluted whole blood, wherein the reagent composition is in dry form and the reagent composition comprises: glucose dehydrogenase (GDH); one or more substances form the group consisting of diaphorase, phenazine methosulphate, phenazine, ethosulphate, phenazine phenosulphate and Meldola blue; one or more substances from the group consisting of NAD, NADP, thio-NAD, thio-NAD, nicotinamide-purine dinucleotide and nicotinamide methylpurine dinucleotide; one or more hemolyzing substances from the group consisting of phospholipase, hemolyzing saponins, saccharides and aliphatic hydrocarbons having 10-16 carbon atoms; and a redox indicator dye.
Claim 6
Dependent Claim
17 words
The reagent composition as claimed in claim 3, wherein the redox indicator dye is a tetrazolium salt.
Claim 7
Dependent Claim
25 words
The reagent composition as claimed in claim 3 wherein the reagent composition comprises: glucose dehydrogenase, diaphorase, NAD, one or more saponins, and a tetrazolium salt.
Claim 8
Dependent Claim
130 words
A reagent composition used to quantitatively determine the glucose concentration of a sample of undiluted whole blood, the reagent composition comprising: glucose dehydrogenase (GDH); one or more substances from the group consisting of diaphroase, phenazine methosulphate, phenazine ethosulphate, phenazine phenosulphate and Meldola blue; one or more substances from the group consisting of NAD, NADP, thio-NAD, thio-NADP, nicotinamide-purine dinucleotide, nicotinamide-methylpurine dinucleotide and nicotinamide-2-chloro-methylpurine dinucleotide; one or more hemolyzing substances from the group consisting of phospholipase, hemolyzing saponins, compounds of hydrophilic mono-, di- or tri-saccharides and aliphatic hydrocarbons having 10-16 carbon atoms; and a redox indicator dye; and which composition is in dry form in a microcuvette, for carrying out quantitative determination of total glucose in undiluted whole blood by transmission spectrophotometry to quantitatively determine the glucose concentration of undiluted whole blood.
Claim 9
Dependent Claim
130 words
A reagent composition used to quantitatively determine the glucose concentration of a sample of undiluted whole blood, the reagent composition comprising: glucose dehydrogenase (GDH); one or more substances from the group consisting of diaphroase, phenazine methosulphate, phenazine ethosulphate, phenazine phenosulphate and Meldola blue; one or more substances from the group consisting of NAD, NADP, thio-NAD, thio-NADP, nicotinamide-purine dinucleotide, nicotinamide-methylpurine dinucleotide and nicotinamide-2-chloro-methylpurine dinucleotide; one or more hemolyzing substances from the group consisting of phospholipase, hemolyzing saponins, compounds of hydrophilic mono-, di- or tri-saccharides and aliphatic hydrocarbons having 10-16 carbon atoms; and a redox indicator dye; and which composition is in dry form in a microcuvette, for carrying out quantitative determination of total glucose in undiluted whole blood by transmission spectrophotometry to quantitatively determine the glucose concentration of undiluted whole blood.
Claim 10
Dependent Claim
26 words
The method as set forth in claim 1 further comprising the step of introducing and freeze drying the reagent in a microcuvette prior to said contacting.
Claim 11
Dependent Claim
16 words
The reagent composition of claim 3 wherein said reagent composition is freeze dried in a microcuvette.
Claim 12
Dependent Claim
11 words
The method of claim 1 wherein the reagent further comprises mutarotase.
Claim 13
Dependent Claim
16 words
The reagent composition as set forth in claim 3 wherein the reagent composition further comprises mutarotase.
Claim 14
Dependent Claim
16 words
The reagent composition as set forth in claim 5 wherein the reagent composition further comprises mutarotase.
Claim 15
Dependent Claim
16 words
The reagent composition as set forth in claim 6 wherein the reagent composition further comprises mutarotase.
References Cited (Backward Citations)
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US4898813A
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| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1997-01-10 | 1997-07-12 | 1998-01-11 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2001-01-10 | 2001-07-12 | 2002-01-11 | $3760.00 | Window Closed / Lapsed |
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