US5334296A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Peroxidase colloidal gold oxidase biosensors for mediatorless glucose determination
Assignee: Magellan Diagnostics Inc
•
Filing: 1993-06-28
•
Granted: 1994-08-02
•
Expires: 2013-06-28
Abstract
Abstract Horseradish peroxidase (HRP) immobilized on colloidal gold and then deposited on an electrode surface can be reduced at a convenient rate at low voltage (Ag/AgCl) without an electron transfer mediator. Bioelectrodes combining both a colloidal gold-adsorbed oxidase and colloidal gold-adsorbed HRP located on an electrode surface are efficient biodetectors, particularly for the measurement of low glucose levels in samples when glucose oxidase is employed as the sensing enzyme. The biodetectors may be employed for mediatorless detection of a wide variety of analytyes depending on the oxidase employed.
Inventors
- 👤 Robert W. Henkens
- 👤 Junguo Zhao
- 👤 John P. O'Daly
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 03:04
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/001; C12Q1/002; C12Q1/003; C12Q1/004; C12Q1/005; Y; Y10; Y10S; Y10S435/00; Y10S435/817
Claims Analysis
Total Claims: 23
Claim 1
Independent Claim
49 words
A method of electrochemical determination of an analyte comprising: obtaining a bioelectrode prepared from a colloidal gold adsorbed peroxidase and an oxidase in communication with a conducting surface; and detecting current generated in the presence of the analyte when a sample containing the analyte is contacted with the bioelectrode.
Claim 2
Independent Claim
49 words
A method of electrochemical determination of an analyte comprising: obtaining a bioelectrode prepared from a colloidal gold adsorbed peroxidase and an oxidase in communication with a conducting surface; and detecting current generated in the presence of the analyte when a sample containing the analyte is contacted with the bioelectrode.
Claim 3
Dependent Claim
25 words
The method of claim 1 wherein hydrogen peroxide from oxidase-catalyzed analyte conversion is selectively reduced by peroxidase on the conducting surface to produce a current.
Claim 4
Dependent Claim
17 words
The method of claim 1 wherein the sample is water, urine, blood, serum, plasma, sweat or tears.
Claim 5
Dependent Claim
33 words
The method of claim 1 wherein the oxidase is glucose oxidase, cholesterol oxidase, xanthine oxidase, amino acid oxidase, alcohol oxidase, lactic acid oxidase, sorbose oxidase, glycolate oxidase, gulonolactose oxidase, pyrodoxal-4-oxidase or galactose oxidase.
Claim 6
Dependent Claim
16 words
The method of claim 1 wherein the analyte is glucose, xanthine, cholesterol, galactose or lactic acid.
Claim 7
Dependent Claim
11 words
The method of claim 1 wherein the peroxidase is horseradish peroxidase.
Claim 8
Dependent Claim
11 words
The method of claim 1 wherein the current is measured amperometrically.
Claim 9
Independent Claim
46 words
A method for mediatorless electrochemical determination of glucose, comprising the steps: obtaining a bioelectrode prepared from colloidal gold adsorbed horseradish peroxidase and glucose oxidase in communication with a conducting surface; and detecting current produced from reduction of hydrogen peroxide generated from glucose oxidase catalyzed glucose conversion.
Claim 10
Independent Claim
46 words
A method for mediatorless electrochemical determination of glucose, comprising the steps: obtaining a bioelectrode prepared from colloidal gold adsorbed horseradish peroxidase and glucose oxidase in communication with a conducting surface; and detecting current produced from reduction of hydrogen peroxide generated from glucose oxidase catalyzed glucose conversion.
Claim 11
Dependent Claim
21 words
The method of claim 8 wherein the detecting of hydrogen peroxide is conducted at a pH of about 4.5 to 7.5.
Claim 12
Dependent Claim
19 words
The method of claim 8 wherein the detecting of hydrogen peroxide is conducted at a pH of about 5.0.
Claim 13
Dependent Claim
19 words
The method of claim 8 wherein the current is measured amperometrically with a transducer comprising a reference/counter electrode component.
Claim 14
Dependent Claim
30 words
The method of claim 9 wherein the reference/counter electrode component is at 0 V/Ag Vs. Ag/AgCl which measures a current generated from direct electron transfer at the conducting electrode surface.
Claim 15
Dependent Claim
26 words
The method of claim 8 wherein current produced is proportional to glucose present in the sample from a range of about 1 to 250 μM glucose.
Claim 16
Dependent Claim
21 words
The method of claim 8 wherein the glucose is measured in a sample of water, blood, vaginal fluid, saliva or semen.
Claim 17
Independent Claim
43 words
A method of determining glucose concentration wherein the glucose is electrochemically determined from a current generated from reduction of hydrogen peroxide produced by glucose oxidase catalyzed glucose conversion, the glucose oxidase being immobilized on a conducting surface with colloidal gold adsorbed horseradish peroxidase.
Claim 18
Independent Claim
43 words
A method of determining glucose concentration wherein the glucose is electrochemically determined from a current generated from reduction of hydrogen peroxide produced by glucose oxidase catalyzed glucose conversion, the glucose oxidase being immobilized on a conducting surface with colloidal gold adsorbed horseradish peroxidase.
Claim 19
Dependent Claim
19 words
The method of claim 15 wherein the glucose oxidase is immobilized on top of a layer of horseradish peroxidase.
Claim 20
Dependent Claim
23 words
The method of claim 15 wherein the glucose oxidase is adsorbed to colloidal gold prior to deposition on the layer of horseradish peroxidase.
Claim 21
Dependent Claim
11 words
The method of claim 15 wherein the conducting surface is carbon.
Claim 22
Dependent Claim
12 words
The method of claim 15 wherein the conducting surface is glassy carbon.
Claim 23
Dependent Claim
15 words
The method of claim 15 wherein immobilization is be evaporative deposition on the conducting surface.
References Cited (Backward Citations)
US4340448A
Cited
US4820399A
Cited
US4970145A
Cited
US5082786A
Cited
WO1991009304A1
Cited
US5082550A
Cited
US5225064A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1997-08-01 | 1998-01-31 | 1998-08-02 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2001-08-01 | 2002-01-31 | 2002-08-02 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2005-08-01 | 2006-01-31 | 2006-08-02 | $7700.00 | Window Closed / Lapsed |